Showing papers in "Biochemical Engineering Journal in 2001"

TL;DR: Medium containing olive oil appears to a better medium for fermentative production of C. cylindracea lipase than yeast extract, malt extract, peptone and tween 80, based on experimental conditions.

TL;DR: The state of the art of culturing microorganisms in shaking bioreactors is reviewed and some issues of their practical application in screening and process development projects are addressed.

TL;DR: The results showed that both the dyes uptake processes followed the second-order rate expression and the equilibrium data fitted very well to both the Freundlich and Langmuir adsorption models.

TL;DR: The oxygen transfer rate (OTR) is the most suitable measurable parameter to quantify the physiological state of a culture of aerobic microorganisms since most metabolic activities depend on oxygen consumption.

TL;DR: The methanolysis of Rhizopus oryzae cells immobilized within biomass support particles (BSPs) for biodiesel fuel production in industrial applications using a whole cell biocatalyst is considered to be promising.

TL;DR: The maximum gas-liquid mass transfer capacity of 250ml shaking flasks on orbital shaking machines has been experimentally investigated using the sulphite oxidation method under variation of the shaking frequency, shaking diameter, filling volume and viscosity of the medium.

TL;DR: In this paper, the authors explored the microbicidal effectiveness of hydrodynamic cavitation for the disinfection of bore well water for potable use and found that hydrodynamical cavitation is an energy efficient and economical technique compared to the other conventional non-chemical water treatment processes and cavitation was found to be a potential physical water disinfection technique for the production of potable water.

TL;DR: The characteristics of polyvinyl alcohol (PVA) and calcium alginate as immobilization matrices were examined and compared for the uptake of gold by a fungal biomass and revealed a much more rapid uptake phenomenon by the former.

TL;DR: The cross-linking immobilization of β-galactosidase from Kluyveromyces lactis on graphite surfaces was investigated, and the deactivation rate was found to follow the Arrhenius law with the activation energy of about 200 kJ mol −1 for the de activation of the immobilized enzyme.

TL;DR: Responding surface methodology was employed to study the cumulative interactive effect of the macronutrients of the media and to optimize their concentration to enhance the production of maltooligosaccharide-forming amylase from Bacillus circulans GRS 313, suggesting that this was the principal experimental variable having the greatest effect on the production.

TL;DR: In this paper, a modified anaerobic baffled bioreactor (MABR) was studied under steady-state conditions for treating palm oil mill wastewater, which corresponded to the methane content of 67.3-71.2% within the range of examined hydraulic retention time (HRT).

TL;DR: In this paper, the ability of dried anaerobic activated sludge to adsorb phenol and chromium(VI) ions, both singly and in combination, was investigated in a batch system.

TL;DR: In this paper, the ability of Rhodococcus sp. P32C1 for desulfurization of n -hexadecane ( n -C16) containing dibenzothiophene (DBT), and two different diesel oils is evaluated.

TL;DR: To avoid the (unidentified) development of a screening project in unfavourable directions or even its complete failure, researchers must be aware of the 'out-of-phase' phenomenon.

TL;DR: A trickling bed biofilm reactor packed with PORAVER particles was used to evaluate phenol and total organic carbon (TOC) removal efficiencies by Pseudomonas putida DSM 548 as discussed by the authors.

TL;DR: This algal system is useful for continuous NO removal and production of algal biomass using NO as a nitrogen source for cell growth and for further application of this system, it is necessary to remove NO over a long duration, and the stability of NO removal is important.

TL;DR: Values of the volumetric oxygen transfer coefficient (kLa), obtained at various impeller speeds, air flow rates and at distinct initial substrate concentrations were correlated with operational variables, geometric parameters of the system and physical properties of the broths utilising rigorous techniques in order to obtain a set of reliable and accurate data.

TL;DR: An agitated 12-well microtiter plate system with a working volume of 2ml was investigated for cell culture process development, which assures homogeneity in wells and enhances mass transfer between the gas and the liquid phase, thus improving maximum cell density and pH stability.

TL;DR: A sandwich spongy silicone/cotton wool cover to close the wells of square-deepwell microtiter plates for aerobic microbial strains and showed a clear influence of the shaking diameter on the hydrodynamic behavior in the wells.

TL;DR: In this paper, a 0.28m diameter slurry bubble column for air-water-yeast cells system was investigated and the effects of gas velocity and yeast concentrations were studied.

TL;DR: Higher concentrations of aerobic cells with higher cellular activities were achieved in fed-batch operated and pH-controlled shaking-flasks as compared to the conventional batch operation.

TL;DR: Hydrodynamic cavitation has been found to release the periplasmic enzyme invertase selectively more selectively than the other two methods used for disruption.

TL;DR: The feasibility of using shake flasks to culture animal cells was evaluated and it was found that conditions can be optimized so that hybridoma, Chinese Hamster Ovary cells, and insect cells can be efficiently cultured in the shaking reactors to cell densities comparable to that obtained with stirred-jar bioreactors.

TL;DR: Glucosamine can be considered as a well-adapted biomass indicator, with the necessity to establish for each medium tested a prior correlation between biomass and glucosamine amount.

TL;DR: In this paper, an automated image analysis procedure was developed for the simultaneous characterisation of flocs and filamentous bacteria (filament length) of fresh activated sludge samples observed by optical microscopy.

TL;DR: Phenol in industrial wastewater has been biologically degraded by Pseudomonas putida immobilized in calcium alginate matrix and an attempt has been made to explain the kinetics of this immobilized whole cell catalytic process on the basis of Michaelis–Menten (M–M) model within the phenol concentration range 100–1000 ppm.

TL;DR: In this article, the ability of Thiobacillus ferrooxidans cultures to reduce chromium(VI) to chromium (III) was evaluated under different conditions.

TL;DR: Results indicate that this decrease in activity was a combination of sub-optimal enzyme activity and variation in the spectrum of proteases secreted under the different pH conditions.

TL;DR: A new method was developed to obtain the mass transfer resistance of various sterile closures using the carbon dioxide diffusion coefficient, which revealed that this resistance is mainly dependent on the neck geometry and to a lesser extent on the plug material and density.

TL;DR: The taxane production titer obtained in this work was the highest as ever reported, and the activity of a key enzyme — taxadiene synthase was greatly increased after elicitation.