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Showing papers in "Biochemical Engineering Journal in 2012"


Journal ArticleDOI
TL;DR: Several bioprocess engineering aspects related to P. pastoris cultivation are discussed, including the different promoters available, both constitutive and inductive, on- and off-line process parameter monitoring methods, fed-batch and continuous cultivation control strategies, proteolytic degradation of products and methods to minimize associated yield reductions, and the different models devised to describe cell growth and protein production.

237 citations


Journal ArticleDOI
TL;DR: In this article, a novel biorefinery concept of utilizing waste bread as a sole nutrient source for the production of a nutrient rich feedstock for the fermentative succinic acid production by Actinobacillus succinogenes has been developed.

211 citations


Journal ArticleDOI
Jingyang Xu1, Xuebing Zhao1, Wencong Wang1, Wei Du1, Dehua Liu1 
TL;DR: Results indicated that crude glycerol could be directly converted to triacylglycerols by R. toruloides without purification, and certain amount of salt and soap could promote the accumulation of biomass and lipid.

177 citations


Journal ArticleDOI
TL;DR: This review presents the “state of the art” in the biotechnological production technologies of 1,3-PD particularly with respect to bioprocess engineering methods and highlights the significance of mathematical model-based approach for designing various bioreactor operating strategies to facilitate the improvement in 1, 3-PD production.

175 citations


Journal ArticleDOI
TL;DR: An insight is given about the various ɛ-PL producing strains, their screening procedures, mechanism of synthesis, characterization, and its application in the medical field.

154 citations


Journal ArticleDOI
TL;DR: Candida antarctica lipase B immobilization by covalent attachment on chitosan and on ch itosan–alginate complex previously activated by different strategies was studied.

130 citations


Journal ArticleDOI
TL;DR: In this article, the authors dealt with the decolourisation and detoxification of pulp paper mill effluent by mixed culture of three bacterial strains having ligninolytic enzyme activity.

127 citations


Journal ArticleDOI
TL;DR: In this paper, the effects of biofilm growth on anode and cathode surfaces on the power generation from wastewater in single chamber microbial fuel cells (SCMFCs) were investigated.

122 citations


Journal ArticleDOI
TL;DR: In this paper, an integrated bio-electrochemical reactor system is described focusing on new configurations, and the impact of important variables, namely, hydraulic retention time, electric current, pH, and carbon source.

120 citations


Journal ArticleDOI
TL;DR: The use of lemon peel pomace and the use of A. niger Aa-20 in SSF suggest as a very promising process for pectinase production.

116 citations


Journal ArticleDOI
TL;DR: In this paper, anode modification was realized by one-step electrochemical treatment in one of the three electrolytes (nitric acid, ammonium nitrate and ammonium persulfate) at ambient temperature.

Journal ArticleDOI
TL;DR: In this paper, the kinetics of bio-leaching of Mo, Ni, and Al from spent hydrocracking catalyst, using Aspergillus niger, were studied, and the four most effective bioleaching variables were selected in accordance with the Plackett-Burman design and were further optimized via central composite design.

Journal ArticleDOI
TL;DR: In this article, the antioxidant activity of 14 lignin samples obtained from apple tree pruning was studied, and different techniques (ultrafiltration, differential precipitation, and Lignin purification) were applied with the aim of improving the lignins properties.

Journal ArticleDOI
TL;DR: In this paper, 15 ionic liquids were evaluated toward their ability to form ABS in the presence of a common biodegradable organic salt: potassium citrate, and the ternary phase diagrams, tie-lines, and respective tie-line lengths, were determined at 25°C.

Journal ArticleDOI
TL;DR: It was shown that C. saccharophila could actively utilize glucose and glycerol as carbon sources and bacteriological peptone as a nitrogen source, and the concentrations of nitrogen and carbon sources that were found to significantly influence the biomass productivity of heterotrophic C.

Journal ArticleDOI
TL;DR: Regardless of the carbon sources used, the fatty acid profile of the microalgal lipid did not change significantly, as the lipid comprises over 60–80% of saturated fatty acids (mainly palmitic acid and stearic acid) and monounsaturated acids (Mainly oleic acid (C18:1)).

Journal ArticleDOI
TL;DR: In this article, a L4 orthogonal array of the Taguchi method was used to optimize the synthesis conditions for obtaining these selective carbons, and the results showed that carbons obtained from pecan nut shells modified with a calcium solution extracted from egg shells were more effective for fluoride removal than those using the nut shell precursor as such.

Journal ArticleDOI
TL;DR: Amending activated sludge with additional microorganisms able to produce versatile enzymes to enhance biodegradability or “bioaugmentation”, is gradually considered as a possible solution for the recalcitrance of Bio-Refractory Organic Compounds (Bio-ROCs).

Journal ArticleDOI
TL;DR: In this paper, an attempt to scale-up a biodiesel production plant from a heterotrophic culture of microalgae, together with an evaluation of feasibility is performed, and the results highlight the future potential of a plant with these characteristics in the current energetic context.

Journal ArticleDOI
TL;DR: The RML-displaying P. pastoris whole-cell biocatalyst exhibited quite a good operational stability with more than 80.46% of its original activity remained even after 10 batches reaction as well as storage stability, which showed promising potential in biodiesel production.

Journal ArticleDOI
TL;DR: In this paper, the authors investigated the effect of the drying air temperature on the functionality of MPC to establish the relationship between drying conditions and MPC microstructures, and found that the protein denaturation increased with increasing drying temperature.

Journal ArticleDOI
TL;DR: Seven different fungal strains from the genera Aspergillus, Mucor, Penicillium, and Neurospora were evaluated to grow and release phenolic compounds from these residues under solid-state cultivation conditions.

Journal ArticleDOI
TL;DR: Microalgae are currently cultivated on a relatively small scale for nutritional products, supplements and aquaculture feed, but there has been renewed interest in algal bioenergy, which would require cultivation at far greater scales as well as carbon capture or sequestration through algal culture.

Journal ArticleDOI
TL;DR: In this article, a new environmentally friendly and efficient approach for isolating cellulose fibers with minimum structural alteration from wood biomass was proposed, which comprised enzymatic delignification of ionic liquid (IL) swollen wood biomass in ILs-aqueous systems with the aim of overcoming low deformation efficiency associated with difficulties in enzyme accessibility to the solid substrate and the poor substrate and products solubility in aqueous system.

Journal ArticleDOI
TL;DR: In this paper, the thermodynamics of waste activated sludge (WAS) hydrolysis process enhanced by additional α-amylase were evaluated based on the analysis of variance (ANOVA).

Journal ArticleDOI
TL;DR: The ability to grow on and degrade phenol at high temperature and low pH is unique and may be useful for removing phenol from hot acidic contaminated effluents and the cloning of the enzymes-related genes in fast-growing mesophiles.

Journal ArticleDOI
TL;DR: The study shows that integration of a properly selected nanofiltration membrane with a cell-recycle fermentation unit and lactate conversion unit can yield l (+) lactic acid with an optical purity of 85.6% through an economically attractive route by recovering and recycling of unconverted sugars.

Journal ArticleDOI
TL;DR: In this paper, the effect of sono-assisted alkali (NaOH) pretreatment on the removal of hemicellulose and lignin from sugarcane bagasse (SCB) was studied and the results showed 80.8% of hemiscale and 90.6% of leignin removal.

Journal ArticleDOI
TL;DR: A method for lactic acid production and lipid extraction from microalgae (Nannochloropsis salina) biomass was investigated in this paper, where the acid was hydrolyzed at 120°C for 1h, and subsequently treated with hexane at 40°C and 200 rpm to separate lipid from the hydrolysate.

Journal ArticleDOI
TL;DR: In this paper, an initial screening by Plackett-burman design was performed to select major variables out of eleven medium components, among which peptone, CuSO 4, and xylidine were found to have significant effects on laccase production.