Showing papers in "Biochimica et Biophysica Acta in 1957"

TL;DR: A new method for the quantitive determination of sialic acids is described, which is about 50% more sensitive than the orcinol-hydrochloric acid method generally used and considerably lower with the resorcin reagent.

TL;DR: In this article, the authors investigated the influence of different cations on the activity of an adenosine triphosphatase (ATP) enzyme in the leg nerves of crabs.

TL;DR: Adaptive increase of the tyrosine-α-ketoglutarate transaminase occurred in the livers of rats following injections of tyrosines or of hydrocortisone, and analysis showed that hydroc Cortisone itself was a sufficient inducing stimulus.

TL;DR: It is shown that active potassium and sodium transport across the human erythrocyte membrane are parts of a single tightly-linked system, independent of extracellular and intracellular sodium and potassium concentrations.

TL;DR: It is found that manganese and magnesium ions have a greater effect than sodium ion in promiting these reactions and it is shown that the two- Stranded complex is more stable than the three-stranded complex.

TL;DR: The method appears to be more specific and more generally applicable than absorption spectrophotometry for the measurement of reduced pyridine nucleotide in vivo.

TL;DR: The mechanism of the electric discharge synthesis of amino and hydroxy acids from CH4, NH3, H2O, and H2 was investigated and β-alanine and succinic acid were probably synthesized by addition of ammonia and hydrogen cyanide to acrylonitrile and hydrolysis to the acids.

TL;DR: The results reported here and elsewhere demonstrate that the amino acid composition of the proteins may be influenced by the presence of exogenous amino acids or their analogs, and suggest that some variation in the amino acids composition of a given protein may be the rule rather than the exception.

TL;DR: In this article, a colorimetric procedure for the determination of thiosulfate in concentration as low as 1.10−5M is described, based on the conversion of thio-sulfate to thiocyanate in the presence of cyanide and cupric ions.

TL;DR: A simple graphical method for the determination of the dissociation constants for two-substrate enzyme systems has been presented.

TL;DR: Findings suggest that the full activity of ribonuclease, in the presence of such denaturing agents, is due to a refolding of the protein under the influence of Ribonucleic acid.

TL;DR: The uptake and esterification were almost abolished by M500 cyanide but were not affected by M20 fluoride, and the significance of the results from the aspect of the mechanism of fat uptake by adipose tissue is discussed.

TL;DR: Urokinase was isolated from urine by adsorption on silica gel and elution with ammonia and was purified by chromatography on IRC 50 at pH 6.2 to show that at higher temperatures urokinases is most stable in the acid range.

TL;DR: Venom from rattlesnake has been studied by chromatography on DEAE cellulose with special interest to phosphodiesterase and DNAase, while lecithinase and l-amino acid oxidase have been followed by semi-quantitative test only.

TL;DR: Absorption optics both in the visible and ultraviolet regions of the spectrum have been used in ultracentrifugal studies at concentrations of about 0.001% and it is shown that a cytochrome boundary behaved ideally and the apparent diffusion coefficient was independent of centrifugal field.

TL;DR: These reactions of the glyoxylate cycle, when coupled with the reactions leading from fat to acetyl CoA, and from malate to carbohydrate, provide a route for the net conversion of fat to carbohydrate.

TL;DR: Comparisons by interference microscopy and chemical analysis prove that at least four-fifths of the myosin in these fibrils is present as the A substance, and the results are in excellent agreement with the hypothesis that all theMyosin is concentrated in the A bands.

TL;DR: An enzyme previously purified from Escherichia coli which catalyzes the conversion of the terminal phosphate of ATP to a highly polymerized polyphosphate has now been shown to catalyze a reversal of this reaction.

TL;DR: The synthesis of long-chain fatty acids from acetate by the reconstituted fractions of pigeon liver supernatant requires the following cofactors: ATP, CoA, GSH, DPNH, Mn++, isocitrate and TPN.

TL;DR: The quantitative relationships indicate that only a fraction of the incorporated DNA may be functional in the usual sense; about 2–10 times the normal cellular content of DNA is incorporated per transformation to streptomycin resistance.

TL;DR: From the behavior in a centrifungal field and other evidence it is concluded that the activity is associated with the bulk of the nucleic acid in the preparations and not with a small fraction of high molecular weight.