Showing papers in "Biochimica et Biophysica Acta in 1960"

TL;DR: A procedure for purification of alkaline phosphatase from E. coli is described, and several physical-chemical propetiies of the purified enzyme are reported.

TL;DR: The observations lend further support to the suggestion made previously that this enzyme is involved in the active, linked transport of Na+ and K+ across the nerve membrane.

TL;DR: Confirmation was presented for the ineffectiveness of 2,4-dinitrophenol and effectiveness of ammonium ions as uncoupling agents, and the lack of exchange between inorganic phosphate and ATP in swiss-chard chloroplasts.

TL;DR: It has been shown that alkaline phosphatase in measurable amount is only formed when Pi becomes limiting in the medium, at which point the enzyme is formed in substantial amount at a maximum rate.

TL;DR: It is concluded that genetic properties of markers, such as size, linkage, and discreteness, are reflections of the actual distribution of determinants within DNA molecules.

TL;DR: A model for the myosin molecule is proposed,based on three equal weight polypeptide chains in the form of a three-stranded α rope, based on X-ray diffraction, light scattering and optical rotatory data.

TL;DR: Malonyl CoA can serve as the starting point for the synthesis of long-chain fatty acids in animal tissues and the isolated enzyme system of cow mammary gland forms fatty acids similar to those found in milk.

TL;DR: The results indicate that at a given temperature the RNA/protein ratio of a cultures is a direct function of the growth rate and that these two variables are linearly related at growth rates greater than 0.6 doublings per h.

TL;DR: The gels are easily regenerated in the columns and may be used daily over a period of months without detectable deterioration, and the column capacity is very high compared to other similar column methods (chromatography and zone electrophoresis).

TL;DR: It has been found that, where discrepancies occur, the limiting viscosity numbers correspond more closely to the molecular weights obtained by light-scattering than to those evaluated from ultracentrifuge data.

TL;DR: Three types of intestinal preparation which exhibit characteristics of active transport similar to those of other in vitro preparations and of active absorption in vivo are described and one kind, in particular, which consists of small rings of everted intestine was shown to be highly reproducible and useful for comparison of the rate and extent of active Transport under various conditions.

TL;DR: It is concluded that phosphatidylserine is decarboxylated to phosphatidylaminoethanol and that the latter compounds is methylated to form lecithin and the significance of these results is discussed in relation to fat and phospholipid metabolism.

TL;DR: Investigation of the intracellular localization of the principal enzymes involved in the formation and breakdown of β-hydroxy-β-methyl-glutaryl CoA in rat liver has shown that HMG CoA condensing and cleavage enzymes are both preponderantly in the mitochondria.

TL;DR: The time relationship between DNA synthesis and mitosis has been determined for L-strain mouse cells cultivated in vitro and a mathematical treatment for exponentially multiplying cultures is presented.

TL;DR: A new method for the purification of bovine erythrocyte carbonic anhydrase shows a high degree of homogeneity in electrophoresis and in the ultracentrifuge and the possible origin of the two forms of the enzyme is discussed.

TL;DR: A low molecular weight RNA has been extracted from whole baker's yeast cells with phenol and compared with yeast S-RNA and several properties which have been investigated are very similar for both products.

TL;DR: In this article, the authors describe a method of isolating and purifying from human milk a mucoid that has, like plasma siderophilin, the ability to bind iron reversibly.

TL;DR: A model is proposed which is compatible with the observed behaviour of the entry and exit parameters under various conditions, and it is shown that the entry mechanism is catalytic; it is coupled with metabolic energy donors.

TL;DR: Kinases responsible for the formation of thymine-deoxyribose-5′-monophosphate, diphosphate and triphosphate from thymine, purified from extracts of Ehrlich ascites carcinoma have been purified.

TL;DR: In this article, a cell-free enzyme preparation of Clostridium pasteurianum was obtained by disrupting frozen cells in a Hughes press or by autolysis of specially dried cells resuspended in phosphate buffer.

TL;DR: A number of previously made band-assignments were confirmed and new suggestions were made for interpreting the bands at 3106, 2962 and 1619 cm −1 .

TL;DR: Preliminary experiments indicated that the mold was capable of converting orotic acid to thymine nucleotides throughout the interphase; this suggests that the control of DNA synthesis must occur at some point very close to the final polymerization of the molecule.

TL;DR: This method permits the determination of N-acetyl groups in a range of 1 to 10 μmoles and is more sensitive than previously described methods.

TL;DR: The spectral properties of two strains of Chlorobium thiosulfatophilum each contain a single, distinct type of chlorophyll and of the correponding pheophytins are described.

TL;DR: Data is obtained about the intestinal absorption of calcium present in food, the rate of the physiological renewal processes of the skeleton through osteolysis and osteoblastosis and the amount of ingested and excreted calcium.