Showing papers in "Biochimica et Biophysica Acta in 1967"

TL;DR: A lethal effect of high electric fields on a number of species of vegetative bacteria and yeasts has been demonstrated, the various species differed in their sensitivity to the electric field, the yeasts being more than the vegetativeacteria.

TL;DR: The size and shape of the particles as well as the degree of birefringence and the extent to which negative stain penetrates between the lamellae during electron microscopy were characteristic of the various phospholipid species used.

TL;DR: The nature and specificity of binding of concanavalin A to cross-linked dextran gels have been shown to be generally similar to that of concavelin A-polysaccharide interaction by demonstrating the reversal of the binding and subsequent elution of the protein from Sephadex G-50 with the same low molecular weight carbohydrates.

TL;DR: The rates of diffusional exchange of trapped ions through hydrated liquid crystals and related vesicular structures composed of various naturally occurring, purified phospholipids were determined and it was concluded that this system constitutes a valid model for many biological membrane phenomena.

TL;DR: It is proposed that the electric field causes an irreversible loss of the membrane's function as the semipermeable barrier between the bacterial cell and its environment, and that this is the cause of cell death.

TL;DR: There are marked species variations in the relative abundance of these erythrocyte phospholipids in several common mammals, including rat, rabbit, pig, dog, horse, sheep, cow, goat, cat and guinea pig.

TL;DR: In this article, the force-area characteristics of monolayers of saturated and unsaturated phospholipids have been studied, and the mean area per molecule in mixed films of cholesterol with (1,2-disteroyl)-3-lecithin and (1 2-didecanoyl) 3-leCIN at 22° has been shown to follow the simple additivity rule.

TL;DR: Rat-liver homogenates were found to hydrolyze phospholipids, giving rise to both the 1-acyil and 2-acyl lysoderivatives, and studies using inhibitors and heat inactivation show that the phospholIPase A2 requires metal ions, that the lipase is sensitive to p-chloromercuribenzoate and that the lysophospholipase(s) are sensitive to deoxycholate and heating.

TL;DR: The treatment would appear to implicate at least 12 structural genes in the biosynthesis of the soluble cavian esterases; a multiplicity of control which is considerably in excess of previous estimations for mammalian sources.

TL;DR: The lack of gangliosides and acetylcholinesterase in synaptic vesicles is emphasized, and the possible physiological significance of these complex acidic glycolipids in some neuronal membranes is discussed.

TL;DR: It has been concluded that these amino acid-specific tRNA's are pure enough to use for structural study, suggesting that methionine-tRNA formed a more rigid secondary structure than other t RNA's.

TL;DR: Anaerobically-prepared bacteroids were able to selectively use substrate-generated reducing power for nitrogen fixation instead of for aerobic respiration, although the pO 2 was greater than that necessary to saturate the terminal respiratory pathway.

TL;DR: A sensitive modification of the luciferase assay for ATP allows samples to be taken rapidly from growing cultures of bacteria without concentrating them from the medium, and the ATP pool in cells grown under carbon-limiting conditions in a chemostat has been measured.

TL;DR: The electrical properties of bimolecular phospholipid membranes separating two aqueous electrolyte solutions were investivated and a steady membrane potential of nearly the limiting value was observed.

TL;DR: The proportion of the normal major monosialoganglioside in myelin was higher than that in whole brain even at 15 days of age, and with increasing age, its proportion increased rapidly reaching 90 mole % of total gangliosides in Myelin by the age of 5 months.

TL;DR: Acyl derivatives of cyclic AMP with substitution on the N6 or 2′-O position or both were more active than the parent compound in producing hyperglycemia in intact dogs and were also more effective in causing phosphorylase activation in liver slices.

TL;DR: All strains of E. coli tested were susceptible to lysis by the neutral detergent Brij-58 and the cellular DNA, after lysis, may be almost completely sedimented by centrifugation at 3000 × g for 5 min.

TL;DR: Fractions obtained after Tris disruption and subsequent density-gradient centrifugation were analyzed for their enzymic and chemical content and found that lactase activity is occasionally found to be differently distributed from the other disaccharidases.

TL;DR: Quantum efficiencies of fiuorescence and emission maxima were measured in a number of solvents for tryptophanyl and tyrosyl peptides, as well as simpler derivatives of indole and phenol, and an α-amino group enhanced the quenching action of a neighboring carbonyl group in the free amino acids and peptides.

TL;DR: A gas-liquid chromatographic method is described whereby all of the monosaccharide residues commonly present in glycoproteins can be identified and estimated simultaneously, after methanolysis and re- N -acetylation.

TL;DR: It is concluded that at least three cytochromes are localized in the chloroplast; cy tochromes f and b 6 with α bands at 554 and 563 mμ respectively, and another cytochrome with an α band at about 559 mμ at 20°C and 77°K.

TL;DR: After depletion of the labile liver protein, muscle protein breakdown has increased and supplied 65% of muscle intracellular amino acid pool, which in time maintained the plasma and tissue amino acid levels.

TL;DR: A fraction rich in plasma membrane has been isolated from perfused rat liver and the purity of the preparation is comparable with that of material isolated by other procedures and the yield of the purified fraction is appreciably greater.

TL;DR: In this article, a mathematical analysis of fluorescence-time curves is followed by a discussion on the nature and efficiency of energy transfers between chlorophyll molecules and photochemical centres.

TL;DR: Partially replicated DNA fragments obtained from the sucrose shelf can be further characterized by subsequent equilibrium sedimentation in CsCl gradients, as consistent with the interpretation that these fragments are Y-shaped partially replicated DNA units from the growing point region of the chromosome.

TL;DR: A great number of proteins were separated, which showed a spectrum of pI's essentially between pH 3 and 11, and α-Haemolysin, deoxyribonuclease, staphylokinase, protease and hyaluronate lyase were found to consist of multiple molecular forms with different pI.