Showing papers in "Biochimica et Biophysica Acta in 1972"

TL;DR: During the first 24 h after injection of 125I-labeled very low density lipoprotein to humans, the decay rate of low densitylipoprotein apoprotein from very lowdensity lipoproteins was faster than that of apolipoprotein glutamic acid, apoipoprotein alanine1 and apolipropoteinAlanine2.

TL;DR: The conductance induced by gramicidin A in lipid bilayer membranes has been shown to be made up of discrete, well-defined units and it has been concluded that the conducting channel is a pore, rather than a carrier.

TL;DR: The addition of small amounts of hyaluronic acid to disaggregated cartilage proteoglyans produced a large increase in hydrodynamic size on gel chromatography and there was no such interaction when proteoglycans were mixed with other polyanions in comparable proportions.

TL;DR: The loss and cleavage are associated with some specific proteins in the polyribosomes, and can be avoided if these proteins are destroyed before extraction or if extractions are done with chloroform plus phenol rather than with phenol alone.

TL;DR: The significance of the change in agglutinability of transformed or trypsinized cells is discussed in relation to the topology of the cell surface.

TL;DR: Observations of small molecular weight apolipoproteins readily transfer in vitro from very low density lipoprotein to other lipoproteins, and this specificity may not be involved in the simple recombination ofApolipoprotein-glutamic acid and apoLP-Ala may explain the distribution of apoprotein among plasma lipopriteins and provide insight into their metabolic fate.

TL;DR: From the flash-induced absorption changes due to the redox reaction of N,N,N′, N′-tetramethyl-p-phenylenediamine coupled with P700, the difference extinction coefficients and the difference spectra constructed from the exponential recovery phases further strengthened the notion that P700 was a form of chlorophyll a.

TL;DR: The procedure for making a suitable gel, application of protein in different ways, detection of protein especially by staining, pH measurement, and sectioning the gel are described.

TL;DR: Semi-quantitative considerations of the univalent cation selectivities lead to the conclusion that there is probably a continuous file of water molecules in the gramicidin pore, and that the binding of ions by the gramICidin does not wholly determine the relative permeabilities.

TL;DR: T4 DNA migrates considerably more rapidly through 4 % agarose than both circular forms of ΦX replicative form DNA (M r 3.4 · 10 6 ).

TL;DR: Analysis of the gangliosides by thin-layer chromatography showed that there was no selective enrichment of any individual type in the plasma membranes in relation to total brain, extending the specific fatty acid compositions observed in grey matter and certain sub-fractions by other workers.

TL;DR: The results make it clear that external calcium or calcium in the transverse tubules play no essential part in action potential propagation or excitation-contraction coupling.

TL;DR: In nuclear magnetic resonance studies, designed to establish the alignment of the probes relative to the membrane lipids, it was found that the signal originating from the choline methyl protons of the membrane phospholipids is broadened by the spin probes; this effect was found to decrease as the paramagnetic ring is farther removed from the polar end of the fatty acid probe.

TL;DR: In this paper, the equilibrium constant of the reaction catalysed by glutamine synthetase ( l -glutamate: ammonia ligase (ADP), EC.1.

TL;DR: The effect of sterols on the permeability properties of liposomes are in good agreement with the effect on the mean molecular area measured in monolayers, and the effect of these sterol on biological membranes such as erythrocytes and Mycoplasma.

TL;DR: The term “viscotropic” is suggested to describe such effects of membrane fluidity on enzyme activity, the ability of phospholipids to exhibit K+Na+ discrimination and current concepts of protein-lipid interactions in biological membranes.

TL;DR: Fat cells which had passed dinonyl phthalate exhibited normal lipogenesis from glucose and normal sensitivity to insulin, and 3H and 14C activity could be removed completely after washing of cells incubated with 3H2O and 3-O-[14C]methylglucose (40 mM).

TL;DR: An analysis of the Arrhenius plots allowing for the energies of activation to be temperature dependent (a slight decrease with increasing temperature) indicates that linearization of the plot would result in erroneously large activation energies.

TL;DR: The unsaturation and the distribution of the double bonds appear to be of critical importance for the barrier properties of lecithins and for the interaction with cholesterol.

TL;DR: The optical and electron paramagnetic resonance (EPR) spectroscopic parameters which characterize the two metal-binding sites of lactoferrin are strikingly similar to those of transferrin, corroborate the suggestion that the transferrins and lact oferrins constitute a single class of proteins evolving from a common ancestral protein.

TL;DR: Ca2+ precipitates of various mixtures of lecithin and cardiolipin display a complex polymorphism between the hexagonal and lamellar phases, which is practically independent of the concentration of the precipitating solution of CaCl2.

TL;DR: It is indicated that ruthenium red has a dual effect on mitochondrial metabolism; it interferes with the binding and transport of Ca2+ and at a slightly higher concentration it markedly inhibits respiration by interacting with the energy coupling pathway.

TL;DR: Investigation of superoxide dismutase activity of bovine erythrocuprein by pulse radiolysis under steady-state and pre-steady-state conditions confirmed that enzyme turnover is effectively diffusion-limited.

TL;DR: Mitochondria from mung bean hypocotyls and potato tubers have been separated from contaminating organelles and membrane fragments on discontinuous sucrose gradients and the influence of purification on oxidative capacities and carrier concentrations is detailed.

TL;DR: Results of the immunological and electrophoretic experiments supported the existence oflipoprotein families as the basic components of plasma lipoprotein system and indicated that lipop Protein families exist above a density range of approximately 1.006-1.019 g/ml as individual separable entities, while at densities less than this range lipop protein families are present primarily as associations.

TL;DR: The enzyme was shown to be inhibited by excess substrate, benzylpenicillin, and by both of the products of hydrolysis, which was found to be competitive and by 6-aminopenicillanic acid to be non-competitive.

TL;DR: The effect of incorporated cholesterol and epicholesterol upon the glycerol and erythritol permeability through the membrane of Acholeplasma laidlawii is studied, suggesting the importance of the 3β-OH group of the sterol molecule for the specific sterol-lecithin interaction.