Showing papers in "Biochimica et Biophysica Acta in 1973"

TL;DR: Single bilayer liposomes, indistinguishable from those obtained by sonication, can be prepared by injecting an thanolic solution of phospholipid into water by injection of dilute suspension.

TL;DR: It is concluded that thisospholipid fraction (which contains the majority of the choline-containing phospholipids and some phosphatidylethanolamine) forms the outer monolayer of the membrane.

TL;DR: It is proposed that, for T c where T c is the transition temperature, perylene is excluded from the hydrocarbon interior of the membranes, whereas, T c this probe may be accommodated in the membrane interior to a large extent.

TL;DR: The finding that prostaglandin R can be converted enzymatically into the biologically inactive prostaglandsin D in good yields, reveals a new pathway in prostag landin metabolism and postulates the key position of prostag Landin R as the first biologically active product formed from essential fatty acids.

TL;DR: A method is described to obtain brush border,i.e. microvillus membranes, from human intestinal epithelial cells based on the sequential use of CaCl2, differential centrifugation and Tris, characterized by a high specific activity in brush border membrane markers as well as its appearance on electron micrographs.

TL;DR: Investigation of the mechanism of a previously reported decreased accumulation of daunomycin in resistant compared to wild-type Ehrlich ascites tumor cells was investigated in vitro, and the effect suggests that these drugs are transported by the same extrusion mechanism as daunaomycin.

TL;DR: A possible mechanism for bend formation (nucleation) is given on the basis of the results from the conformational energy calculations carried out on the pentapeptide N-acetyl N′-methyl Ala-Ala-AlA-Alas amide.

TL;DR: This procedure, compared with the conventional chloroform-methanol procedure, was proved to achieve a more complete extraction of gangliosides with no concurrent solubilization of glycoproteins and to guarantee the absence of any significant degradation.

TL;DR: O2−· persists long enough to diffuse as far as the chloroplast envelope and tonoplast, which are the first structures to be damaged by paraquat treatment, and is sufficiently long-lived to be a candidate for the phytotoxic product formed by paraaquat in plants.

TL;DR: The aminopeptidase which has been purified accounts for all, or almost all, of the peptidase activity of the brush border membrane of porcine jejunum and ileum.

TL;DR: It is discussed that the light-dependent alkalization in the chloroplast stroma may be involved in the regulation of CO2 fixation at the fructose diphosphatase step.

TL;DR: Visibly normal specimens from the superficial layer which were situated adjacent to visibly degenerate cartilage were weaker and less stiff than specimens situated on normal joints or remote from visibly degenerated cartilage.

TL;DR: The procedure for the isolation of highly purified sarcoplasmic reticulum vesicles from rabbit skeletal muscle has been described using sucrose gradient centrifugation in zonal rotors and enrichment in the level of phosphoenzyme by the Ca 2+ -pump protein was regarded as an important index of the purification of sarcoplasts.

TL;DR: A new concept for the haemolytic behaviour of mixtures ofospholipases is proposed, based upon a highly asymmetric distribution of the phospholipids in a bimolecular leaflet, and taking into account some aspects of the action of phospholIPases towards red cell ghosts.

TL;DR: The results indicate the presence of two carrier-mediated uptake systems for choline, one of which requires Na+ for the uptake and the other does not.

TL;DR: Two isozymes of the cupro-zinc superoxide dismutase in wheat seeds remain unknown, and both were found in the seed heads of a commonly-grown non-hybrid strain of wheat, whereas the leaves and stalk of that strain contained only one of them.

TL;DR: This paper gives a treatment of ion transport through pores based on the theory of reaction rates, which allows for saturation effects in the pore, and shows that certain information on the single rate constants may be obtained from stationary conductance measurements.

TL;DR: Observations suggest that concomitant with continuous triglyceride hydrolysis, apoLP-glu and apo LP-ala leave the very low density lipoprotein density range resulting in molecules relatively poor in triglyceride and relatively rich in apoLDL.

TL;DR: The biological significance of these data are discussed in relation to the possible effects of increased cholesterol levels in cell membranes during the process of aging and the development of atherosclerosis.

TL;DR: The results show that the principle binding of insulin by fat cells occurs to a group of receptors with a Kd of 3 · 10−9 M and that the degradation of insulin and the receptor binding are processes independent of each other, suggesting that the stimulatory effect of insulin on lipogenesis from glucose in fat cells is mediated by these receptors.

TL;DR: The oxygen equilibrium and the absorbance spectrum of the oxygenated form were successfully measured with myoglobin and hemoglobins A and M Boston and it was found that the addition of catalase or peroxidase was required for the complete reduction of metmyoglobin.

TL;DR: A rapid, simple and sensitive radioassay for phenolsulfotransferase (EC 2.8.2.1) activity is described based on the transfer of 35SO4 from phosphoadenosine 5′-phospho[35S]sulfate to a phenolic acceptor, suitable for use with the phenolic catecholamine metabolites found in brain.

TL;DR: It was found that the basal microsomal ATPase ( i.e. without K + ) and the mitochondrial ATPase were inhibited by SCN − and enhanced by HCO 3 − , however, the K + -stimulated component of the microsome ATPase was virtually unaffected by these anions.

TL;DR: It is concluded that only one of the many chloroplast soluble proteins, namely the large subunit of Fraction I protein, is synthesised on chloropleft ribosomes.

TL;DR: Reaction center particles isolated from carotenoidless mutant Rhodopseudomonas spheroides were studied with the aim of determining the pigment composition and the molar extinction coefficients, and revised values are shown to be compatible with those obtained from measurements of fluorescence transients.

TL;DR: The results clearly indicate the existence of a mosaic-like structure of the membrane with the whole enzyme system being enclosed by a rather rigid phospholipid halo, which is in a quasicrystalline structure below 32 °C and undergoes a crystalline-liquid crystalline phase transition at32 °C.

TL;DR: The results indicated that the hydroxyprolyl residues preceding glycyl residues in the peptides stabilize the triple-helical structure, and they suggested therefore that hydroxy Proline may perform a similar function in collagen.