Showing papers in "Biochimica et Biophysica Acta in 1977"

TL;DR: A molecular model on the action of local anesthetic-like antiarrhytmic drugs is formulated: the drugs can interact with the sodium channels in the rested, activated and inactivated states, and each of these interactions is characterized by an association and dissociation rate constant.

TL;DR: A unified theory is developed that explains the formation of bilayers and vesicles and accounts quantitatively for many of their physical properties: properties including vesicle size distributions and bilayer elasticity emerge from a unified theory that links thermodynamics, interaction free energy, and molecular geometry.

TL;DR: CM-Cellulose column chromatography proved to be extremely useful in separating phospholipid mixtures obtained by phosphatidyltransferase reactions of phospholIPase D and is also suitable for fractionation of other lipid extracts.

TL;DR: The Raman technique is extended through an examination of the origins of the structure-sensitive features of phospholipid spectra and the development of quantitative order-parameters to find that the vesicles of dipalmitoyl phosphotidylcholine are substantially less ordered than the dispersions in terms of both longitudinal and lateral order.

TL;DR: Standard experimental procedures for continuous polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate were modified to give more effective separation and improved resolution of myofibrillar proteins.

TL;DR: The isolate of prostaglandin endoperoxide synthetase requires haemin, which possibly acts as an easily dissociable prosthetic group, and a suitable hydrogen donor to protect the enzyme from peroxide inactivation and which is consumed in stoichiometric amounts to reduce the intermediate hydroperoxy group.

TL;DR: It is concluded that the key event leading to vesicle membrane fusion is the isothermic phase change induced by the bivalent metals.

TL;DR: Isotonic suspensions of human erythrocytes were exposed to single electric pulses of intensity at a few kV/cm and duration in microseconds, and the cell membranes became permeable to Na+ and K+ and hemolysed through the colloid osmotic effect of hemoglobin.

TL;DR: The affinity order of cholesterol for the neutral phospholipids which can be deduced is sphingomyelin greater than phosphatidylcholine greater thanosphatidylethanolamine.

TL;DR: The results suggest that triazines, triazinones, pyridazinone derivatives, biscarbamates and phenylureas interfere with the same electron carrier of the photosynthetic electron transport chain, according to the same molecular mechanism.

TL;DR: Human liver extracts show two major bands with aldehyde dehydrogenase (Aldehyde:NAD+ oxidoreductase, EC 1.1), very similar to the F1 and F2 isozymes of horse liver purified by Eckfeldt et al.

TL;DR: Results suggest that in platelets, as in muscle, cyclic AMP is a regulatory factor in the control of cytoplasmic calcium.

TL;DR: A simple method is presented for calculating the parameters of the hole model for distorted octahedral, low spin (t2g)5 complexes, with explicit formulas for the coefficients of the Kramer's doublet, where g defines the plane and orientation of the orbital with the largest coefficient, which indicates the directions of maximal unpaired spin density.

TL;DR: New information is discussed about the mechanism of lipid and apoprotein interaction in the plasma lipoproteins and a special form of the amphipathic helix has been identified as a major structural element of the apolipoproteins sequenced to date.

TL;DR: The annulus fibrosus of human lumbar discs as a whole contained a considerably higher proportion of type II collagen than did pig annulus Fibrosus, and this distribution appears to be a characteristic structural feature of this tissue.

TL;DR: The data are interpreted in favour of a role of calcium as the second messenger in liver for the three cyclic AMP-independent glycogenolytic hormones and as an additional messenger for glucagon which, via cyclicAMP, will make calcium available to the cytoplasm either from extracellular or from intracellular pools.

TL;DR: It is shown that the use of low cation media enhances the separation of intact and broken chloroplasts in a new method of obtaining chloroplast retaining their outer membranes.

TL;DR: Changes in the cortical proteins in advanced nuclear cataractous lenses are probably the result of simple oxidation of the proteins with O2 or H2O2, suggesting that the oxidation spreads from the nucleus to the cortex.

TL;DR: These studies indicate the presence of at least three major components in HDL instead of two (HDL2 and HDL3) and that peak F degrees 1.20 rate differences in HDL schlieren patterns between males and females are a function of the relative levels of these three components.

TL;DR: It is concluded that the quinones serve to link the various dehydrogenases with the terminal electron transport systems to oxygen and nitrate and that the dehydrogenase possess a degree of selectivity with respect to the qu inone acceptors.

TL;DR: Investigating the action of seven purified phospholipases on intact platelets (both from human and pig) has revealed that membraneospholipids are available to enzymatic breakdown only if the phospholIPase can produce degradation in monolayers spread at an initial surface pressure of at least 34 dynes/cm.

TL;DR: A fast and efficient method for the separation of (phospho)lipids by high performance liquid chromatography using n-hexane, 2-propanol, water mixtures as the solvent system is described.

TL;DR: In this article, the specificity of porcine pancreatic elastase toward peptide substrates was analyzed by examining cleavages of peptides of known sequence which were reported in the literature.

TL;DR: This technique allows preparation of both highly stable water-insoluble enzymes (on formation of gel cross-linked with N,N′-methylene bisacrylamide) and soluble stabilized enzymes (if polymerization is performed without other than the enzyme cross-linking agents).

TL;DR: The glycerol ethers are proposed to be unique fully saturated diglycerol tetraethers, primarily C86H172O6, Mr 1300, which contain two sn-2,3-glycerol residues bridged through ether linkages by two C40 isopranoid branched diols.

TL;DR: The data suggest that double strand breaks are repaired in human fibroblasts, and no significant differences were found between fibro Blasts from two normal donors and four patients with the radiosensitive disorder, ataxia telangiectasia, in either the production or repair ofdouble strand breaks.