scispace - formally typeset
Search or ask a question

Showing papers in "Biochimica et Biophysica Acta in 1980"


Journal ArticleDOI
TL;DR: The effective hydrophobicity determined fluorometrically showed significant correlations with interfacial tension and emulsifying activity of the proteins studied.

1,238 citations



Journal ArticleDOI

952 citations


Journal ArticleDOI
TL;DR: Investigations suggest that as compared to 4-hydroxynonenal very low amounts of other 4-Hydroxyalkenals are formed by actively peroxidizing liver microsomes, and this finding may help to elucidate the mechanism by which lipid peroxidation causes deleterious effects on cells and cell constituents.

713 citations


Journal ArticleDOI
TL;DR: The yield of gangliosides obtained by extraction of the tissue twice with twenty volumes of chloroform/methanol/water was larger than that obtained with all other solvents tested, including tetrahydrofuran/phosphate buffer.

704 citations


Journal ArticleDOI
TL;DR: The lateral heterogeneity in the distribution of the photosystems between appressed and non-appressed membranes must have a profound impact on current understanding of both the distributionof excitation energy and photosynthetic electron transport between the Photosystems.

629 citations



Journal ArticleDOI
TL;DR: The procedure is relatively simple, rapid, and yields almost quantitative recovery of vesicles that encapsulate a large percentage of the total aqueous space.

484 citations


Journal ArticleDOI
TL;DR: A cotransport system for Na+, K+ and Cl- in Ehrlich cells is described and appears to function in cell volume regulatin as it tends to make the cell swell, thus counteracting the shrinking effect of the ouabain-sensitive (Na+, K+) pump.

477 citations


Journal ArticleDOI
TL;DR: Efflux of contents from small unilamellar vesicles of various compositions, conaining a highly quenched fluorescent compound was determined as a function of temperature in the presence and absence of human serum.

467 citations


Journal ArticleDOI
TL;DR: Electrodes showed a Nernstian response to [Ca2+] down to 1 micro M free concentration in 0.1 M KCl, and usually a useful response to below 100 nM Ca2+.

Journal ArticleDOI
TL;DR: The microscopic phase configuration of simple bilayer systems is markedly affected by the relative concentration of sphingomyelin, phosphatidylcholine and cholesterol, and it is clear that the properties of bilayers comprised of these two superficially similar phospholipids reflect differences in molecular structure.

Journal ArticleDOI
TL;DR: A stimulus receptor mechanism for physical forces which, like hormonal effectors, are mediated by prostaglandins and stimulate cyclic AMP and DNA synthesis is suggested and it is believed that physical forces stimulate bone remodelling through such a stimulus receptor system, mediated by Prostaglandin E2.


Journal ArticleDOI
TL;DR: It appears that a variety of treatments, namely, chelating drugs, transition series metals, sulfhydryl reagents, heat shock, and amino acid analogous can induced similar, if not identical, proteins in eukaryotic cells.


Journal ArticleDOI
TL;DR: Results with the oxonol on both mixed lymphocytes and B cell-enriched suspensions gave confirmation of the conclusions from diS-C3-(5) experiments and indicated that despite its biological side effects, diS+ could still give valid assessment of membrane potential.

Journal ArticleDOI
TL;DR: The findings suggest that S-nitrosothiols could act as intermediates in the activation of guanylate cyclase by glyceryl trinitrate, NaNO2 and possibly nitroprusside.

Journal ArticleDOI
TL;DR: The time sequence of the appearance of the spin-trapped superoxide and the semiquinone radical metabolite of these quinone-containing anticancer drugs indicates that air oxidation of the semiquesinone is responsible for the superoxide formation.

Journal ArticleDOI
TL;DR: It is proposed that two essential interactions are responsible for the complex stabilization: an electrostatic interaction between the protonated amino groups of the sugar residues and the ionized phosphate residues, and an interaction between adjacent anthraquinone chromophores.



Journal ArticleDOI
TL;DR: Rabbit intestinal brush border membrane vesicles were prepared either from frozen or fresh tissue and the lipid composition was analysed, finding individual phospholipids are characterised by distinct fatty acid compositions and differ markedly in the ratio of unsaturated-to-saturated fatty acid.

Journal ArticleDOI
TL;DR: The addition of cholesterol results in changes in the ester bands, and demonstrates the induction by cholesterol of non-equivalent ester conformations, in the absorptions characteristic of the phospholipid head groups.

Journal ArticleDOI
TL;DR: The removal of iron was studied in buffers in the pH range 8-3 varying the ionic strength and with or without metal chelators such as phosphate ions and EDTA to demonstrate differences between the stability of the two iron-binding sites.

Journal ArticleDOI
TL;DR: Results indicate assembly of large subunits into ribulose bisphosphate carboxylase in the sorbitol medium and time course experiments indicate that there is a time-lag of several minutes between onset of synthesis of largeSubunits and the onset of assembly.

Journal ArticleDOI
TL;DR: It is proposed that the bacteriochlorophyll c (BChl c; chlorobium chlorophyll) is located in the rod elements of the core and that it is complexed with specific proteins.

Journal ArticleDOI
TL;DR: Calmodulin has been purified to homogeneity using an improved procedure that allows rapid processing of several kilograms of bovine brain and it was determined that calmodulin is not present as a soluble protein in Escherichia coli.


Journal ArticleDOI
TL;DR: It is concluded that vanadate is reduced by cytoplasmic glutathione in this concentration range and that the reduction explains the resistance of the (Na+ + K+)-ATPase to vanadium in intact cells.