Showing papers in "Biochimica et Biophysica Acta in 1981"

TL;DR: It was concluded that the shedded vesicles constituted a select portion of the plasma membrane and may serve a physiologic function; it is proposed that they be referred to as exosomes.

TL;DR: In this paper, an interpretation of fluorescence polarization measurements in lipid membranes which are labelled with the apolar probe 1,6-diphenyl-1,3,5-hexatriene is presented.

TL;DR: High order and statistically significant correlations between densitometric scans of the (HDL2b)gge, ( HDL2a) gge and (HDl3)g Ge material, as obtained from gradient gels, and plasma concentrations of the HDL2b, HDL2a and HDL3 components, as obtaining from analytic ultracentrifugation were demonstrated.

TL;DR: Rat kidney cortex slices were homogenized with a polytron in a isoosmotic medium containing 5 mmol/l EGTA and by SDS-polyacrylamide gel electrophoresis, no differences in the protein patterns were observed.

TL;DR: This ‘salting out’ method was applied to enterotoxigenic Escherichia coli strains with different surface protein antigens, found to have a surface hydrophobicity in the following order: CFA/I >CFA/II > K88∼K99 > type 1.

TL;DR: It is suggested that a large proportion of proteoglycans from normal disc have undergone a degree of degradation in the hyaluronate binding region and that proteoglycan synthesis in this tissue is slower than in degenerate tissue.

TL;DR: 6'-amidino-2-naphthyl-4-guanidinobenzoate dihydrochloride, 4-(beta- amidinoethenyl)phenyl- 4-guAnidin Obenzoates dimethanesulfonate and 4-amidinos2-benzoylphenyl the most effective inhibitors of trypsin, plasmin, pancreatic kallikrein and thrombin.

TL;DR: An analysis of the photo-induced decline in the in vivo chlorophyll a fluorescence emission (Kautsky phenomenon) from the bean leaf is presented and an inverse relationship was found between the proportion of PS II electron acceptors in the oxidised state and PS IIfluorescence emission.

TL;DR: Impurities in 5(6)-carboxyfluorescein can affect phospholipid vesicle stability and apparent rates of carboxy fluoresceins transfer into cells, so thorough purification and characterization of the dye are important to many applications with vesicles and/or cells.

TL;DR: It is suggested that the role of phospholipids in membrane fusion is related to their ability to form dehydrated intermembrane complexes with divalent cations.

TL;DR: The identification of two separate strains of MDCK cell with entirely different properties are described and the polypeptide composition of the apical membrane differs substantially between the two cell strains as revealed by radio-iodination of external membrane proteins.

TL;DR: In a new approach for the determination of polypeptide conformation, experimental data on intramolecular distances between pairs of hydrogen atoms obtained from nuclear Overhauser enhancement studies are used as input for a distance geometry algorithm that determines the limits of the conformation space occupied by the polyPEptide chain.

TL;DR: The data indicate that in addition to composition, the lipid dose, total liposomal surface area and effective mean diameter are important pharmacokinetic variables and the optimization of the therapeutic index of an encapsulated agent or target-tissue delivery via liposomes will require consideration of both the surface areaand diameter of the liposome doses together withliposome composition.

TL;DR: Comparison spectra with beef heart mitochondria and with cell suspensions of Saccharomyces cerevisiae revealed that myxothiazol blocked the electron transport within the cytochrome b-c1 segment of the respiratory chain.

TL;DR: In order to further investigate the cooperation between ascorbate and vitamin E in suppressing lipid peroxidation, a liposomal system containing polyunsaturated phospholipids was used and the mixture of antioxidants was much more effective than the sum of both vitamins alone.

TL;DR: The results suggest that carnitine palmitoyltransferase less sensitive to malonyl-CoA is exposed on the outer surface of the inner mitochondrial membrane in fasting, thus reducing the latency of the enzyme.

TL;DR: Saturation of the high affinity lysine-binding site in plasminogen with tranexamic acid had no significant influence on the solubilization rate of fibrin, indicating that binding of pl asminogen to fibrIn is of little importance for its activation by urokinase.

TL;DR: Liposomes, in the size range of 40--180 nm, are formed when lipid and additives are solubilized with detergent, yielding defined mixed micelles, and the detergent is subsequently removed by controlled dialysis.

TL;DR: Modification of the enzyme to expose certain monosaccharide moieties results in increased delivery to specific cell types and naturally occurring receptors can be utilized for targeting glucocerebrosidase to the non-parenchymal cell in liver.

TL;DR: Zn .