Showing papers in "Biochimie in 1992"

TL;DR: This manuscript summarizes research results from the laboratory regarding the role of endogenous proteases in post mortem proteolysis resulting in meat tenderization and indicates that the calcium-dependent proteolytic system (calpains) is responsible for post mortam proteolyses.

TL;DR: Activity of the calpains in living cells is regulated by calpastatin and Ca2+, but the nature of this regulation is still unclear.

TL;DR: These and other regulatory aberrations in tumor cells appear to be reflections of a complex set of non-random phenotypic changes, initiated by expression of oncogenes.

TL;DR: A new integrational vector is constructed, pKSV7, which contains temperature-sensitive replication functions derived from pE194ts and allows the identification of inserts in the polylinker cluster by disruption of alpha-complementation and thus should be useful for other kinds of genetic manipulations in B subtilis.

TL;DR: The presence of surfactin, at a concentration at which, alone, it is inactive, increases to a very large extent the haemolysis percent induced by iturin A, this synergistic effect seems to be in relation with interactions between itur in A and surfact in mixed monolayers.

TL;DR: Evidence is provided suggesting that the substrate, namely myofibrils, might constitute an important limiting step of the efficiency of both types of mechanism, and that improvement of knowledge in this field will greatly depend on the development of basic research on these different topics.

TL;DR: The results presented are consistent with a model in which the phosphorylated form of Spo0A acts directly at its promoters to achieve induction of Ps and repression of Pv, and were independent of the presence of the major kinase, KinA.

TL;DR: The results suggest the existence of a special repressor/activator regulatory system which controls mRNA distribution between free mRNPs and polyribosomes in rabbit reticulocytes and suggest that p50 has a dual role in protein biosynthesis.

TL;DR: The results show that the high 2DG6P and low ATP concentrations found in the steady state of the perfused heart should strongly reduce the rate of phosphorylation of sugars by hexokinase.

TL;DR: The effect of curing agents (salt, nitrate, ascorbic acid and glucose) and processing parameters (pH, water activity and drying and cooking temperatures) on pork muscle cathepsins B, D, H and L as well as leucyl, arginyl and tyrosyl hydrolysing activities is reported.

TL;DR: A number of growth trials using the agonists cimaterol and clenbuterol with cattle, sheep, chicken and rat are reported which suggest a general mechanism whereby beta-agonists reduce calpain I activity, but increase calpain II and calpastatin activity in skeletal muscle.

TL;DR: The proposed essentiality of the hbs gene product for growth and development in B subtilis may be attributed to its interaction with replication and transcription as a consequence of its facility to wrap DNA and to condense the chromosome into nucleosomelike structures.

TL;DR: In vitro mutagenesis of the region revealed that although murD and murG are essential for normal vegetative growth, spoVE is only required for sporulation: spoVE null mutants display a sporulation stage V phenotype indistinguishable by light microscopy from the phenotype conferred by the spoVE85 and spoVE153 alleles that originally defined the locus.

TL;DR: The increase upon ageing in levels of 34- and 35-kDa peptides, often considered as the tropomyosins, suggests that these compounds are myofibrillar degradation products, and Troponin-T was degraded very fast in the early ageing period.

TL;DR: Glial cells synthesized more amino acids, ie, their TCA cycle was used to a larger extent for biosynthesis than is the case of neurons, where it is preferentially used for the energy metabolism.

TL;DR: The natural calpain activator specifically acts on calpain II isoform, by reducing the Ca2+ required for the autoproteolytic activation process, and its role in the expression of specific cell functions can be postulated.

TL;DR: The essential molecular mechanisms of these phenomena, and of structural changes in connectin or titin filaments, are reported, which are non-enzymatically induced by 0.1 mM calcium ion, which is the ultimate concentration of sarcoplasmic calcium ion in post mortem muscles.

TL;DR: It is found that the spoIIN locus is identical with the B subtilis counterpart of the Escherichia coli ftsA gene, and the FtsA protein is therefore required for proper cell septation, both during vegetative growth and sporulation.

TL;DR: It is concluded that the transpeptidation reaction per se triggers conformational changes in the ribosomal complex bringing the 3'-end of a newly synthesized peptidyl-tRNA nearer to the peptidol-site of the peptIDyltransferase center.

TL;DR: To examine the molecular mechanisms that control the expression of the Bacillus subtilis recA gene, a deletion analysis of the recA promoter region was performed and a regulatory region that is required for repression of recA expression was identified upstream of the RecA promoter.

TL;DR: Immunochemical analyses revealed that both murine T- and B-lymphocytes display a capping of the lectin receptors on their cell surfaces after lectin treatment, and Pellina lectin at concentrations between 0.3 and 10.0 micrograms/ml potently enhances interleukin 1 (IL-1) release from mouse peritoneal macrophages and interLEukin 2 ( IL-2) production in mixed murine lymphocyte cultures.

TL;DR: Research on nuclear gene products interfering with mtDNA or with its gene products is the next step towards understanding the etiology of these diseases.

TL;DR: Using the SANS contrast variation method the radii of gyration of protein and RNA components in both ribosomal subunits as well as the intersubunit distance in the pre- and post-translocation states were determined and suggest the following model of the ribosome as a dynamic machine.

TL;DR: In this article, different xyloglucan fractions were extracted from Rubus fruticosus cells cultured in suspension, and two distinct Xoglucans existed in the primary walls.

TL;DR: Altered glycosylation of ras-transformed NIH3T3 is the result of a competitive effect of the enzymes acting for terminal glycosolation of N-linked glycans and the reflection of the higher expression of α-2,6-sialyltransferase.

TL;DR: The calculated curvature profiles predict two bend centers in monomers of each satellite, resulting in a model of left-handed solenoid superstructures of similar appearance, and predict that P ratzeburgii satellite DNA forms a left- handed solenoids superstructure.

TL;DR: The results of three recent studies carried out in the laboratory are developed and concern the direct quantitative monitoring of the hepatic metabolism of FU in the isolated perfused mouse liver, the elucidation of the origin of the cardiotoxicity of F U and the metabolism of IF from an analysis of biofluids of patients.

TL;DR: Calpains digest dystrophin very rapidly when the calcium concentration is compatible with their activation, and these observations suggest that dyStrophin antibodies specific to the central domain of the molecule should be used to detect dystophin for diagnostic purposes.