Showing papers in "Bioconjugate Chemistry in 1995"

TL;DR: Hydrogels with two different types of carboxyl group locations were employed as substrates containing the carboxylic acid, while ethylenediamine and benzylamine were used as amine to study the mechanism of amide formation between carboxYlic acid and amine in aqueous media using 1-ethyl-3-(3-(dimethylamino)propyl)carbodiimide hydrochloride.

TL;DR: A large cross-section of degradable polymeric delivery systems for protein and peptide pharmaceuticals, including monolithic type devices in which the drug is dispersed throughout the polymer and protein-polymer conjugates, are reviewed.

TL;DR: A comparison ofmPEG- and mPEG2-modified enzymes (ribonuclease, catalase, asparaginase, trypsin) was carried out for activity, pH and temperature stability, Km and Kcat values, and protection to proteolytic digestion.

TL;DR: The results of this study suggest that the copolymer complex increases the sequence-specific inhibition effect of oligo on the virus reproduction.

TL;DR: It is concluded that FPLL-DNA complexes are delivered into KB cells via folate receptor-mediated endocytosis and likely follow a lysosomal pathway into the cytoplasm.

TL;DR: The conjugation of three amine reactive fluorescent probes, each containing the fluorophore fluorescein but different reactive moieties, was compared using the protein myoglobin and the amino acid L-lysine as reagents to demonstrate relative performance and stability.

TL;DR: A DNA delivery system based on the use of polylysine substituted with small recognition signals, such as carbohydrate moieties specifically recognized by membrane lectins present in a given cell line, has been developed and the strength of the electrostatic interactions between the plasmid and the glycosylatedpolylysine plays an important role in the efficiency of the gene expression.

TL;DR: It is demonstrated here that surface-modified diamond nanoparticles (5-300 nm) provide conformational stabilization, as well as a high degree of surface exposure to protein antigens, for mussel adhesive protein (MAP), a substance for which conventional adjuvants have proven only marginally successful in evoking an immune response.

TL;DR: A heterobifunctional crosslinker was synthesized from a diamine derivative of poly(ethylene glycol) (PEG, average molecular weight 800 Da), with the functional groups 2-(pyridyldithio)propionyl (PDP) and N-hydroxysuccinimide ester (NHS).

TL;DR: In this article, the peptide-carrying liposomes, with up to 500 YIGSR residues per vesicle, despite exhibiting faster blood clearance rates than the parent Liposomes in rats, remained in circulation for extended periods of time.

TL;DR: Well-defined poly(ethylene oxide) (PEO) with a formyl group at one end and a hydroxylgroup at the other terminus was synthesized by the anionic ring opening polymerization of ethylene oxide with a new organometallic initiator possessing an acetal moiety, potassium 3,3-diethoxypropyl alkoxide.

TL;DR: Well-defined alpha-methoxy-omega-amino and a alpha-hydroxy-omegala-allyl PEOs were obtained after chemical modifications of anionic polymerization of ethylene oxide with allyl alcoholate as initiator by controlling molecular weights of the prepolymer by the monomer/initiator ratio.

TL;DR: The results suggest that bacteriophage encapsulation and targeting is an efficient way to deliver toxins in a cell-specific fashion and may have widespread application in the field of targeted drug delivery, including antisense reagents.

TL;DR: The results suggest that low molecular weight glycopeptides may be suitable for further development as well-defined DNA carriers for receptor-mediated gene delivery in vivo.

TL;DR: In vitro and in vivo testing of the conjugates showed that their bioactivity was substantially maintained and that they were actively transported in an intrinsic factor dependent fashion across CaCo-2 cells and from the intestine to the circulation in a biologically active form.

TL;DR: A strategy for the synthesis of novel oligodeoxynucleotide (ODN)-peptide conjugates on a scale suitable for the investigation of their potential as antisense inhibitors of gene expression and the interaction of the ODN-peptid conjugate with its complementary strand was similar to that of unmodified oligonucleotides.

TL;DR: The definition of the optimal pH and temperature conditions leading to an efficient synthesis of peptide-oligonucleotide conjugates is defined: the reaction was quantitative at pH 7.5 within few hours and the conjugate with a thioether bond has a higher antiviral activity than the peptides-free oligonucleotide and the Conjugate linked via a disulfide bond.

TL;DR: The stannous reduction of [99mTc]pertechnetate in the presence of tricine results in the formation of the new labeling precursor complex [[99m-Tc]-tricine, which has improved efficacy for the 99MTc labeling of hydrazinonicotinate-modified IgG compared to [99MTC]glucoheptonate.

TL;DR: These VB12-ANTIDE conjugates show potential utility as water soluble ANTIDE analogues for parenteral use and are protease resistant LHRH antagonist analogues suitable for uptake from the intestine via the VB 12-transport system following oral administration.

TL;DR: Experiments establish that uptake of this conjugate by human hepatocellular carcinoma (Hep G2) is cell-type specific when compared with its uptake by human fibrosarcoma and human promyleocytic leukemia.

TL;DR: Covalent high-resolution patterning of the laminin fragment CDPGYIGSR to hydroxylated fluorinated ethylene propylene (FEP-OH), poly(vinyl alcohol), and glycophase glass has been achieved.

TL;DR: The presence of PEG on the surface of liposomes is shown to provide an effective method of inhibiting aggregation and the corresponding increase in liposome size during the covalent coupling of avidin-D.

TL;DR: Well-defined poly(ethylene oxide) (PEO) with a cyano group at one end and a hydroxyl group at the other terminus was synthesized by the anionic ring opening polymerization of ethylene oxide (EO) initiated with (cyanomethyl)potassium with metalation reaction of acetonitrile with potassium naphthalene in THF.

TL;DR: Reversibility of pharmacologic action of thiolated peptide was demonstrated by icv naloxone administration, and the cleavability of the disulfide linker in vivo in rat plasma and brain was assessed.

TL;DR: Optimum labeling efficiencies were obtained through application of these principles and the macrocyclic chelators chelated yttrium-90 more slowly in a concentration-dependent manner where efficiency was maximal only when the chelator:metal ratio was greater than 3.

TL;DR: A simple one-step procedure is found to be highly effective for the "functionalization" of glycodiversity, and the inherent amino group provides a convenient site for chemoselective conjugation and modification.

TL;DR: The energy transfer enhanced ion luminescence of the derivatives containing a para-amino-substituted phenyl ring showed particularly strong dependence on environmental changes, which makes these derivatives well suited for homogeneous time-resolved fluoroimmunoassay based on the use of external luminescent modulators.