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JournalISSN: 0178-515X

Bioprocess Engineering 

Springer Science+Business Media
About: Bioprocess Engineering is an academic journal. The journal publishes majorly in the area(s): Fermentation & Bioreactor. It has an ISSN identifier of 0178-515X. Over the lifetime, 1361 publications have been published receiving 26113 citations.


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Journal ArticleDOI
Jong Hyun Choi1, Sang Yup Lee1
TL;DR: The production cost of poly(3-hydroxybutyrate) (PHB) can be considerably lowered when agricultural wastes, such as whey and molasses, are used, and the cost of carbon substrate significantly affected the overall economics in large production scale.
Abstract: Several processes for the production and recovery of poly(3-hydroxybutyrate) (PHB) by Alcaligenes eutrophus, Alcaligenes latus, Methylobacterium organophilum, and recombinant Escherichia coli were designed based on the previously reported data and analyzed by computer-aided bioprocess design. PHB productivity, content, and yield significantly affected the final price of PHB. For the annual production of 2,850 tonnes of purified PHB, the process employing A. eutrophus with the recovery method of surfactant-hypochlorite digestion resulted in lowest price of PHB, $ 5.58/kg. As the production scale increased to one million tonnes per year, the price of PHB dropped to $ 4.75/kg. The cost of carbon substrate significantly affected the overall economics in large production scale. Therefore, the production cost can be considerably lowered when agricultural wastes, such as whey and molasses, are used.

436 citations

Journal ArticleDOI
TL;DR: This analysis examines the motion of microcarriers or free cells relative to the surrounding fluid, to each other, and to moving or stationary solid surfaces in a microcarrier or suspension bioreactor to determine the primary mechanisms of cell damage.
Abstract: Tissue cells are known to be sensitive to mechanical stresses imposed on them by agitation in bioreactors. The amount of agitation provided in a microcarrier or suspension bioreactor should be only enough to provide an effective homogeneity. Three distinct flow regions can be identified in the reactor: bulk turbulent flow, bulk laminar flow, and boundary-layer flows. Possible mechanisms of cell damage are examined by analyzing the motion of microcarriers or free cells relative to the surrounding fluid, to each other, and to moving or stationary solid surfaces. The primary mechanisms of cell damage appear to result from (a) direct interaction between microcarriers and turbulent eddies, (b) collisions between microcarriers in turbulent flow, and (c) collisions against the impeller or other stationary surfaces. If the smallest eddies of turbulent flow are of the same size as the microcarrier beads, they may cause high shear stresses on the cells. Eddies the size of the average interbead spacing may cause bead-bead collisions which damage cells. The severity of the collisions increases when the eddies are also of the same size as the beads. Bead size and the interbead distance are virtually equal in typical microcarrier suspensions. Impeller collisions occur when the beads cannot avoid the impeller leading edge as it advances through the liquid. The implications of the results of this analysis on the design and operation of tissue culture bioreactors are also discussed.

280 citations

Journal ArticleDOI
TL;DR: IPTG induction of a recombinant protein was shown to influence important cell parameters and considerably increased the yield of carbon dioxide per glucose added, indicating an increased maintenance in the large-scale bioreactor.
Abstract: A heterogeneous micro-environment was identified in a 12 m3 bioreactor with a height-to-diameter ratio of 2.5. The reactor was aerated by a ring sparger and stirred by three Rushton turbines. E. coli cells were cultivated in minimal medium to a cell density in the order of 30 g/l. Samples of glucose, the growth limiting component fed to the process, were taken at three levels in the bioreactor (top/middle/bottom). These showed that glucose concentration declined away from the feedpoint. The gradients depended on the mixing characteristics of the feedpoint, and concentrations of up to 400 times the mean value were found when feed was added to a relatively stagnant mixing zone. This resulted in up to 20% lower biomass yield compared to the bench scale. Gradients also affected the by-product formation, resulting in acetate formation in the large-scale bioreactor. IPTG induction of a recombinant protein was shown to influence important cell parameters and considerably increased the yield of carbon dioxide per glucose added, indicating an increased maintenance. The product formation rate was, however, not notably affected by the scale-up.

215 citations

Journal ArticleDOI
TL;DR: In this article, microorganisms were isolated for the elimination of a number of xenobiotics, e.g. aromatic compounds and chlorinated hydrocarbons, in order to make bio-filtration applicable on a larger scale in process industry.
Abstract: Biofiltration is a technique which is frequently applied for the odour abatement of waste gases. This technique is based on the ability of microorganisms (generally bacteria, and to a small extent moulds and yeasts) to degrade several organic as well as inorganic compounds to mineral end-products, like water and carbon dioxide. In the case of biofiltration, microorganisms are attached to suited packing materials in the filter, which contain the inorganic nutrients necessary for microbial growth. In order to make biofiltration applicable on a larger scale in process industry, it is necessary to find microorganisms able to eliminate compounds which are strange to life, the so-called xenobiotics. At the Eindhoven University of Technology microorganisms were isolated for the elimination of a number of xenobiotics, e.g. aromatic compounds and chlorinated hydrocarbons.

194 citations

Journal ArticleDOI
TL;DR: Chitin, a homopolymer of N-acetylglucosamine, is obtained from a variety of sources and forms the structural component of fungal cell wall and plants.
Abstract: Chitin, a homopolymer of N-acetylglucosamine, is obtained from a variety of sources. They form the structural component of fungal cell wall and plants. They are commercially obtained from shrimp and crab shell waste from the fishing industry. Recent advances in understanding the structure and properties of chitin and its derivatives has opened a lot of new avenues for its applications. Improvements in the properties of chitin for a particular application can be easily brought about by chemical modifications. The applicability of chitin in many areas and its easy manipulation has resulted in a considerable amount of research being done on the possible applications of chitinase.

184 citations

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Performance
Metrics
No. of papers from the Journal in previous years
YearPapers
20213
20206
20194
20186
201718
201317