Showing papers in "Biosensors and Bioelectronics in 2004"

TL;DR: A mediator-less microbial fuel cell was used as a biochemical oxygen demand (BOD) sensor in an amperometric mode for real-time wastewater monitoring and the original current value was regained with a varying recovery time depending on the length of the starvation.

TL;DR: The key steps of each analytical procedure, namely DNA-probe immobilisation, hybridisation, labelling and electrochemical investigation of the surface, are discussed in detail with separate sections devoted to label-free and newly emerging magnetic assays.

TL;DR: An approach to detect the hybridisation of DNA sequences using electrolyte-oxide-semiconductor field-effect transistors (EOSFETs) with micrometer dimensions and results indicate that the sensor output is charge sensitive and distance dependent from the gate surface, which pinpoints the need for very defined surface chemistry at the device surface.

TL;DR: Cyclic voltammetric results with assembled MWNT electrode indicate that the strategy based on the LBL method for assembling the MWNT multilayer films on substrate well retains the electrochemical catalytic activity of theMWNTs toward ascorbic acid and dopamine.

TL;DR: An equivalent electrical circuit, consisting of double layer capacitors, a dielectric capacitor, and a medium resistor, was introduced and used for interpreting the change in impedance during bacterial growth, and it had effect on the impedance measurement.

TL;DR: It is observed that disassembly of barrier-forming tight junctions precedes changes in cell-substrate contacts and correlates strongly with the time course of protease activation.

TL;DR: The proposed sensor was comparable to Protein A-based piezoelectric immunosensor in terms of the amount of immobilized antibodies and detection sensitivity.

TL;DR: Direct detection of non-amplified genomic DNA from infectious agents is afforded through increased specificity and even identification of single nucleotide polymorphisms (SNP) in human genomic DNA appears feasible.

TL;DR: HMS provided a good matrix for protein immobilization and biosensor preparation and its direct electrochemistry displayed an excellent response to the reduction of both hydrogen peroxide (H2O2) and nitrate (NO2-).

TL;DR: The experimental comparison shows that the relative sensitivity of the magnetoresistive biosensor is superior to the fluorescent detection at low probe DNA concentrations.

TL;DR: A novel microfabricated device to simultaneously and actively trap thousands of single mammalian cells in alignment with a planar microelectrode array and addresses many needs to manipulate and register cells to the surfaces of biosensors and other devices with high precision and fidelity.

TL;DR: Spectroscopy analysis of the Hb/ZrO2/DMSO film showed that the immobilized Hb could retain its natural structure and the electrocatalytic activity to the reduction of hydrogen peroxide (H2O2) without the aid of an electron mediator.

TL;DR: An immunosensor based on surface plasmon resonance (SPR) using protein G was developed for the detection of Salmonella typhimurium with detection range of 10(2) to 10(9)CFU/ml and could be applied to construct other immnosensors or protein chips.

TL;DR: This study demonstrated that broad-range salivary amylase activity monitor was developed that could be used with only 5 microl of saliva and was possible to use for the analysis of the salivaries without the need to determine thesalivary volume quantitatively.

TL;DR: Preparation and basic characterization of polypyrrole-based molecularly imprinted polymer (MIP) for label-free detection of bovine leukemia virus (BLV) glycoprotein gp51 (gp51) is firstly described and seems to be very effective for the future development of mPpy-based MIPs.

TL;DR: Using gold electrodes lithographically fabricated onto microscope cover slips, DNA and proteins are interrogated both optically (through fluorescence) and electronically (through conductance measurements) for resistance measurement.

TL;DR: Experimental tests show that the implementation of the microfluidics reduces the response time of the sensor from 100s, as achieved with a bulky cuvette, to 4s, resulting into higher accuracy and offering the prospect of being able to use the kinetics of the immunoreaction.

TL;DR: It is confirmed that DNA sequence can be detected by measuring the variation of the drain current due to the variationof DNA charge and the proposed FET-type DNA charge sensor might be useful in the development for DNA chips.

TL;DR: The aim of this work is to demonstrate the potential of the integrated calorimetric microfluidic device for fundamental thermodynamic studies in biochemical reactions and using arrays of such devices with immobilized enzymes multi-analyte detection can be accomplished and the effects of interferents from competing substrates can be compensated.

TL;DR: This study develops a detailed understanding of the influences of various biosensor design parameters in order to enhance the sensitivity and detection limit capabilities of such devices.

TL;DR: Under optimal conditions, the proposed glucose ENFET exhibits a linear response with glucose in the range of 0.025-1.90 mM, an extended dynamic upper limit of 3.5 mM glucose, and considerable good reproducibility and stability.

TL;DR: Results indicated that PEG interfaces created in this investigation are appropriate for biosensors with micro- and nano-scale features, and are efficient in controlling protein fouling.

TL;DR: The results demonstrated that the use of a biosensor with a specific aptamer as bio-recognition element could be an interesting approach in the detection of proteins, which has been here examined considering a model system.

TL;DR: With this method, CRP is quantitatively detected within 10 min in one microliter of human plasma down to concentrations of 30 ng ml(-1), which suggests the possibility to detect CRP at clinically relevant concentrations for the management of coronary heart disease (CHD) and systemic inflammation.

TL;DR: A novel integrated bio-sensor technology based on thin-film bulk acoustic wave resonators on silicon is presented and the feasibility of detecting DNA and protein molecules proofed and the measurement results show the new bio-Sensor being capable of both, detecting proteins as well as the DNA hybridisation without using a label.

TL;DR: The development and characterisation of implantable polyimide microprobes that allow simultaneous, selective chemical delivery/probing and multi-channel recording/stimulation of bioelectric activity are reported on.

TL;DR: It is shown that it is possible to quantitatively study the binding of PSA to the surfaces of the magnetic particles and thus to determine the PSA concentration in solution containing known concentration of nanoparticles functionalised with a monoclonal PSA antibody.

TL;DR: The results show that this method has good correlation for detection of Hepatitis B virus surface antigen in the range of 0.5-200 microg/l and a detection limit of about 50 ng/l.

TL;DR: The detection of L. monocytogenes with a polyclonal antibody by means of a new subtractive inhibition assay using a BIAcore 3000 biosensor has the potential for the quick and sensitive detection of pathogens with limited sample handling and preparation.

TL;DR: A new biochip consisting of an array of microelectrodes with fully-integrated analog and digital circuitry realized in an industrial CMOS process addresses signal degradation and array size issues, thereby facilitating simultaneous stimulation and recording of electrogenic cell activity.