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JournalISSN: 0736-6205

BioTechniques 

Future Science Ltd
About: BioTechniques is an academic journal published by Future Science Ltd. The journal publishes majorly in the area(s): DNA & Polymerase chain reaction. It has an ISSN identifier of 0736-6205. It is also open access. Over the lifetime, 7225 publications have been published receiving 273993 citations. The journal is also known as: BioTechniques: the International Journal of Life Science Methods.


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Journal ArticleDOI
TL;DR: The extraction of DNA from semen and very small bloodstains using Chelex 100 is as efficient or more efficient than using proteinase K and phenol-chloroform extraction and DNA extracted from bloodstain seems less prone to contain PCR inhibitors when prepared by this method.
Abstract: Procedures utilizing Chelex 100 chelating resin have been developed for extracting DNA from forensic-type samples for use with the PCR. The procedures are simple, rapid, involve no organic solvents and do not require multiple tube transfers for most types of samples. The extraction of DNA from semen and very small bloodstains using Chelex 100 is as efficient or more efficient than using proteinase K and phenol-chloroform extraction. DNA extracted from bloodstains seems less prone to contain PCR inhibitors when prepared by this method. The Chelex method has been used with amplification and typing at the HLA DQ alpha locus to obtain the DQ alpha genotypes of many different types of samples, including whole blood, bloodstains, seminal stains, buccal swabs, hair and post-coital samples. The results of a concordance study are presented in which the DQ alpha genotypes of 84 samples prepared using Chelex or using conventional phenol-chloroform extraction are compared. The genotypes obtained using the two different extraction methods were identical for all samples tested.

5,838 citations

Journal ArticleDOI
TL;DR: This research presents a novel and scalable approach to genome engineering that addresses the challenge of integrating RNAseq data to provide real-time information about the “silent” response of the immune system to DNA editing.
Abstract: White1, J. Li1, W. Liang1, N. Bhagabati1, J. Braisted1, M. Klapa1, T. Currier1, M. Thiagarajan1, A. Sturn1, M. Snuffin2, A. Rezantsev2, D. Popov2, A. Ryltsov2, E. Kostukovich2, I. Borisovsky2, Z. Liu3, A. Vinsavich3, V. Trush3, and J. Quackenbush1,4 1The Institute for Genomic Research, Rockville, MD, 2DataNaut, Bethesda, MD, 3Syntek Systems, Bethesda, MD, and 4Department of Biochemistry, George Washington University, Washington, D.C., USA

4,756 citations

Journal Article
TL;DR: The complete recovery of DNA from samples used for the RNA and protein isolation makes it possible to normalize the results of gene expression studies based on DNA content instead of on the more variable total RNA, protein content or tissue weight.
Abstract: This report describes a new method for simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. The method is based on the use of a reagent containing phenol and guanidine thiocyanate. A biological sample is homogenized in the reagent and the simultaneous isolation of RNA, DNA and proteins is accomplished in a single step by a liquid-phase separation. The isolation of RNA can be completed in about 1 h, and DNA and proteins in about 3 h. The simultaneously isolated RNA, DNA and proteins are ready for Northern, Southern and Western blotting. The complete recovery of DNA from samples used for the RNA and protein isolation makes it possible to normalize the results of gene expression studies based on DNA content instead of on the more variable total RNA, protein content or tissue weight.

2,807 citations

Journal Article
TL;DR: A set of murine retrovirus-based vectors that include unique cloning sites for insertion of cDNAs such that the cDNA can be driven by either the retroviral long terminal repeat, the immediate early promoter of human cytomegalovirus, or the simian virus 40 early promoter are described.
Abstract: We describe a set of murine retrovirus-based vectors that include unique cloning sites for insertion of cDNAs such that the cDNA can be driven by either the retroviral long terminal repeat, the immediate early promoter of human cytomegalovirus, or the simian virus 40 early promoter The vectors carry the neomycin phosphotransferase gene expressed from an alternate promoter as a selectable marker These vectors have been constructed to prevent viral protein synthesis from the remaining viral sequences, to yield high-titer virus stocks after introduction into retrovirus packaging cells, and to eliminate homologous overlap with viral DNAs present in retrovirus packaging cells in order to prevent helper virus production Methods for generating high-titer virus are described

2,090 citations

Journal ArticleDOI
TL;DR: The near-comprehensive range of import filters that allow easy access to image and meta-data, a broad suite processing and analysis routine, and enthusiastic support from a friendly mailing list are invaluable for all microscopy labs and facilities-not just those on a budget.
Abstract: of short add-on programs to provide additional functionality to the core program. These additional files are either written in Java (the plugins) or in ImageJ’s macro programming language (macros). Once saved to the ImageJ plugins folder, these functions are loaded on start-up and can be accessed via menu commands like any other core function. 400+ PLUGINS

1,775 citations

Performance
Metrics
No. of papers from the Journal in previous years
YearPapers
202342
202278
202135
2020110
201999
201895