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JournalISSN: 0951-208X

Biotechnology Techniques 

Springer Science+Business Media
About: Biotechnology Techniques is an academic journal. The journal publishes majorly in the area(s): Immobilized enzyme & Fermentation. It has an ISSN identifier of 0951-208X. Over the lifetime, 1797 publications have been published receiving 24755 citations.


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Journal ArticleDOI
TL;DR: A new semi-quantitative agar plate test for the detection of extracellular rhamnolipids has been developed and can be applied to other glycolipid producing microorganims.
Abstract: A new semi-quantitative agar plate test for the detection of extracellular rhamnolipids has been developed. These biological anionic tensides (biosurfactants) form an insoluble ion pair with the cationic tenside cetyltrimethylammonium bromide and the basic dye methylene blue which was included in mineral agar plates. On the light blue agar, productive colonies ofPseudomonas spec. were surrounded by dark blue halos. The test is specific for anionic biosurfactants and can be applied to other glycolipid producing microorganims.

402 citations

Journal ArticleDOI
TL;DR: There was a significant relationship between in vivo fluorescence of cells stained with Nile Red and lipid content in B. braunii determined gravimetrically, suggesting that the Nile Red staining as a rapid method was as good as the gravimetric method commonly used for lipid determination which requires toxic solvents and considerable time-consuming manipulations.
Abstract: Of various methods for lipid recovery in Botryococcus braunii UTEX 572, the most effective method was disruption of the cells with a bead-beater followed by extraction with chloroform/methanol (2:1, v/v). This gave a lipid content of 28.6% of dry wt. There was a significant relationship between in vivo fluorescence of cells stained with Nile Red and lipid content in B. braunii determined gravimetrically (r2 = 0.997). This suggested that the Nile Red staining as a rapid method was as good as the gravimetric method commonly used for lipid determination which requires toxic solvents and considerable time-consuming manipulations. © Rapid Science Ltd. 1998

375 citations

Journal ArticleDOI
TL;DR: An improved adaptation of the direct transesterification method of Lepage and Roy for the preparation of fatty acid methyl esters allows notable saving of time and reagents.
Abstract: An improved adaptation of the direct transesterification method of Lepage and Roy (J. Lipid Res. 25, 1391–96, 1984) for the preparation of fatty acid methyl esters allows notable saving of time and reagents. The material being analysed is heated for 10 minutes with methanol, acetyl chloride and hexane. © Rapid Science Ltd. 1998

331 citations

Journal ArticleDOI
TL;DR: Chelation solubilization of gellan under mild conditions has been accomplished for the first time by exposure to either 10 mM sodium citrate buffer or to 1 mM sodium hexametaphosphate buffer.
Abstract: Chelation solubilization of gellan under mild conditions has been accomplished for the first time by exposure to either 10 mM sodium citrate buffer (pH 6.0) or to 1 mM sodium hexametaphosphate (pH 6.6). The citrate system was preferred for most applications since its is a ubiquitous cellular component, its solutions are autoclavable, and because viable plant tissues, fungi, and bacteria could be recovered from culture. Such recovery is not possible from more commonly used media such as agar.

226 citations

Journal ArticleDOI
TL;DR: The BIAcore biosensor was used to detect binding of Escherichia coli O157:H7 with specific antibodies and has potential for use in rapid, real-time detection and identification of bacteria, and to study the interaction of organisms with different antisera or other molecular species.
Abstract: The BIAcore biosensor was used to detect binding of Escherichia coli O157:H7 with specific antibodies. Immobilized Protein A or Protein G captured antibodies which in turn bound to the bacteria. Alternatively, immobilized antibody captured the E. coli O157:H7 and the bacteria were further probed by a second antibody which enhanced the signal. The regenerated sensor surfaces were used for at least 50 separate analyses. The surface plasmon resonance biosensor has potential for use in rapid, real-time detection and identification of bacteria, and to study the interaction of organisms with different antisera or other molecular species. © Rapid Science Ltd. 1998

209 citations

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Performance
Metrics
No. of papers from the Journal in previous years
YearPapers
1999178
1998197
1997212
1996192
1995178
1994176