Showing papers in "British journal of pharmacology and chemotherapy in 1966"
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TL;DR: The proposed structure has been confirmed by synthesis and the name of phyllokinin has been suggested for the new naturally occurring bradykinin-like endecapeptide.
Abstract: Phyllomedusa rohdei, a small Brazilian amphibian, contains in its skin, in addition to a number of inactive polypeptides, at least three peptides active on plain muscle. The first one (polypeptide a) is characterized by a stimulant action on the rat uterus and the rat colon combined with a moderate hypotensive action in the dog; the second (polypeptide b) by a typical bradykinin-like activity; the third (polypeptide c) by a physalaemin-like activity (cf. Bertaccini, Cei & Erspamer, 1965a, 1965b). The three polypeptides may be separated from each other by chromatography on alkaline alumina column followed by elution with descending concentrations of ethanol. Polypeptide a emerges from the column in the 95% ethanol eluates, polypeptide b in the 70% ethanol eluates, and polypeptide c in the 60% and 50% ethanol eluates. Polypeptide a is possibly a tryptophan-containing pentapeptide, the synthesis of which is in progress; polypeptide c has not yet been obtained in a pure state; polypeptide b has been isolated and identified as bradykinyl-isoleucyl-tyrosine 0-sulphate (Anastasi, Erspamer, Bertaccini & Cei, unpublished). The proposed structure has been confirmed by synthesis (Bernardi, Bosisio, De Castiglione & Goffredo, 1966). For the new naturally occurring bradykinin-like endecapeptide the name of phyllokinin has been suggested.
83 citations
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