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Showing papers in "British journal of pharmacology and chemotherapy in 1967"









Journal ArticleDOI
TL;DR: The concentrations of prostaglandins present in 23 types of human tissues was estimated from necropsy examinations of adult patients carried out within 24 hours, and all tissues were macroscopically free of disease.
Abstract: The concentrations of prostaglandins present in 23 types of human tissues was estimated. Tissues were obtained from necropsy examinations of adult patients carried out within 24 hours. All tissues were macroscopically free of disease and in most instances were examined histologically to confirm this. The preparation used was shown to be sensitive to about 0.5 ng/ml. prostaglandins E1 E2 and F2alpha and 2 ng/ml. F1alpha. The recovery of known amounts of added prostaglandins of the different types which were subjected to the standard extraction and separation procedures and then assayed was found to be between 46 and 59 percent.

127 citations






Journal ArticleDOI
TL;DR: In the cat, angiotensin and bradykinin release catecholamines from the adrenal medulla but eledoisin does not, and the observations on the releasing actions of these peptides are extended and compared with cat and dog.
Abstract: In the cat, angiotensin and bradykinin release catecholamines from the adrenal medulla (Feldberg & Lewis, 1964, 1965), but eledoisin does not (Staszewska-Barczak & Vane, 1965; Lewis & Reit, 1966). The present experiments extend the observations on the releasing actions of these peptides and compare their effects in cat and dog. Some of the results were communicated to the Physiological Society (Staszewska-Barczak & Vane, 1964).









Journal ArticleDOI
TL;DR: The identification of prostaglandins E1 E2 F1alpha and F2alpha in extracts of umbilical blood vessels shows the distribution of these substances outside the genital sphere and raises the issue of their possible physiological role.
Abstract: The identification of prostaglandins E1 E2 F1alpha and F2alpha in extracts of umbilical blood vessels is described. Umbilical arteries and veins isolated from about 80 fresh cords were split along their longitudinal axis and washed with distilled water to remove the blood. Due to its high sensitivity guinea-pig proximal colon was used for the routine bioassay of prostaglandins extracted from biological tissues and fluids. The responses of this preparation to the 4 prostaglandins isolated from the umbilical blood vessels are shown in figure form. Fractionation of the final ether extract of umbilical cord vessels on silicic acid column resulted in 4 peaks of biological activity. The main part of the biological activity was present in peaks 2 and 3 eluted with ethyl acetate:benzene (60:40 and 80:20 respectively). The identification of prostaglandins in the umbilical cord vessels shows the distribution of these substances outside the genital sphere. The discovery of these substances in the umbilical and placental vessels raises the issue of their possible physiological role. Because of the action of prostaglandins E2 F1alpha and F2alpha in causing the isolated umbilical artery preparation to contract it is possible that they play a role in the closing of the cord vessels after the fetus is expelled.