Showing papers in "Cell in 1974"

TL;DR: The conversion of 3T3-L1 from pre-adipose to adipose cells therefore involves a process of differentiation which can be studied under cell culture conditions.

TL;DR: Two clonal sublines are isolated from the established mouse fibroblast line 3T3 that accumulate large amounts of triglyceride fat when the cells are in the resting state and are reduced by lipolytic agents.

TL;DR: It is suggested that the degree of cell growth in methyl cellulose is a useful parameter in vitro for predicting tumorigenicity in the animal, and the potential usefulness of the nude mouse for analysis of cellular malignancy irrespective of the tissue or species of origin is demonstrated.

TL;DR: Individual segments of the chromosomal DNA in D. melanogaster were isolated, and the sequences they contain were analyzed for repetition and mapped within the polytene chromosomes, which are not compatible with tandem repetition models for the chromomere.

TL;DR: Cell-cell hybridization experiments utilizing OUA R and wild-type CHO cells indicate that resistance to ouabain behaves as a codominant trait, and that this marker can be useful for selection of somatic cell hybrids.

TL;DR: A very low level of methylation is detected in heterogeneous nuclear RNA, suggesting that methylation, like polyadenylation, may constitute a post-transcriptional modification of messenger RNA precursor in eucaryotic cells.

TL;DR: The data indicate that poly(A) − mRNA is not short-lived nuclear or cytoplasmic heterogeneous RNA contamination, and that the half-life of the poly( a) − RNA may parallel that of thePoly(A), + mRNA, and the method of RNA fractionation precludes contamination.

TL;DR: Using a method for the rapid determination of binding capacity and subcellular distribution of radioactive dexamethasone by cultured lymphoma cells, quantitative steroid-binding experiments suggest that certain receptor-containing variants possess altered receptor molecules.

TL;DR: The doubling of mRNA content that takes place before any cells begin to synthesize DNA should be considered in relation to the absence of change in the overall rate of synthesis of HnRNA during the same period.

TL;DR: The selection of auxotrophic mutants of Chinese Hamster Ovary cells and the genetic and biochemical characterization of two mutant lines are described and it is suggested that a possible function of polyglutamyl residues is to aid cellular retention of folates.

TL;DR: The large, external, transformation-sensitive (LETS) glycoprotein of hamster fibroblasts, detected by lactoperoxidase-catalyzed iodination, is shown to vary in its accessibility at the cell surface, depending upon the growth state and position in the cell cycle.

TL;DR: The first measurement has been made of the number of diverse mRNA sequences (mRNA sequence complexity) in the total polysomes of a eucaryotic system, the sea urchin gastrula, and the amount of hybrid could be used as a direct measure of the mRNA sequence complexity.

TL;DR: The conclusion that repetitive and nonrepetitive sequences are intimately interspersed in the majority of the DNA of the sea urchin, Strongylocentrotus purpuratus, suggests that this interspersion pattern is of general occurrence and selective importance.

TL;DR: Infection by avian sarcoma viruses of subgroups A, B, and C, but not avian leukosis viruses of groups A and B, elevates the level of plasminogen activators produced by the fibroblasts, and the zymogens trypsinogen, chymotryps inogen, and pepsinogen cannot be activated.

TL;DR: Increasing cell density, and therefore number of cell contacts in untreated cultures, causes the morphology to change in the direction to be expected if the cellular concentration of cyclic AMP is increased.

TL;DR: Some of the numerous effects of this drug on the behavior of cells and viruses may be due to a deoxycytidineless state, rather than to the incorporation of the bromodeoxyuridine into DNA.

TL;DR: Pseudo-diploid and pseudo-tetraploid cultured mouse lymphoma cells are killed by physiological concentrations of adrenal glucocorticoid hormones and the chromosome number, growth rate, gross morphology, and cloning efficiency of the steroid-resistant variants are not detectably different from those of their steroid-sensitive parents.

TL;DR: The location within these molecules of oligo(U) fragments and of transcripts of repetitive DNA sequences has been determined by studies of the shorter 3′ poly(A) terminated sequences of HnRNA produced by cleavage with alkali.

TL;DR: It is suggested that the activity of the spo T gene product is regulated by some consequence of downshift, and plays a role in the phosphorylation of ppGpp to pppGpp preparatory to further metabolism.

TL;DR: It is concluded that the tumorigenic C cells are the stem cells of this teratocarcinoma line, and that they give rise to nontumorigenic E cells in vitro, but that the reverse does not occur.

TL;DR: Steroid binding has been studied in cytoplasmic extracts of normal mouse kidney and of kidney from mice affected with testicular feminization that have inherited androgen resistance, and it is concluded that Tfm kidney contains very little, if any, androgen receptor with properties similar to that found in normal kidney.

TL;DR: Four new type C viruses were isolated from putatively virus-negative baboon lung, kidney, and testicular cells by cocultivation with several permissive host cell lines and represent a distinct new class of endogenous primatetype C viruses.

TL;DR: The results agree with predictions based on the idea that mutations in the structural gene for the enzyme are responsible for the drug-resistant character of these variants.

TL;DR: Results suggest that the normal glucocorticoid receptor has two active domains: one for steroid binding, and the other for interaction with nuclear acceptor sites, and that the resistant cells contain a receptor apparently defective in the latter activity.

TL;DR: Spontaneous mutants of cultured Chinese hamster cells deficient in APRT have been isolated by selection in 8-azaadenine and exhibit stable phenotypes, and the frequency of fully deficient cells in a partially deficient population is enhanced by treatment with a mutagen.