Showing papers in "Cell in 1977"

TL;DR: This paper describes an alternate procedure for the first dimension which, unlike isoelectric focusing, resolves basic as well as acidic proteins, and involves a short time of electrophoresis toward the cathode and separates most proteins according to their isoelection points.

TL;DR: A simple and sensitive method for detecting, sizing and mapping RNA transcripts from viral or cloned DNAs has been developed and used to examine the cytoplasmic transcripts produced during the early phase of adenovirus 2 (Ad2) infection of HeLa cells.

TL;DR: The results prove the usefulness of transfection assays as a means for the bioassay and isolation of restriction fragments carrying specific genetic information and cells expressing HSV-1 tk may also provide a useful model system for the detailed analysis of eucaryotic and viral gene regulation.

TL;DR: Findings imply a new mechanism for the biosynthesis of Ad2 mRNA in mammalian cells which is complementary to sequences within the Ad2 genome which are remote from the DNA from which the main coding sequence of each mRNA is transcribed.

TL;DR: It is presented that crown gall tumors are caused by the incorporation of part of a virulence plasmid carried by the inciting bacterium, Agrobacterium tumefaciens, and the relationship between this plant tumor and virally induced animal tumor systems is discussed.

TL;DR: A scaffolding model for metaphase chromosome structure is suggested in which a backbone of nonhistone proteins is responsible for the basic shape of metaphase chromosomes, and the scaffold organizes the DNA into loops along its length.

TL;DR: A new approach to study differentiation antigens by means of monoclonal xenogeneic antibodies produced by myeloma-hybrid lines in culture is described, which allows identification down to minor membrane molecules and also of antigen on small subpopulations of a heterogeneous mixture of cells.

TL;DR: Several lines of evidence are presented in support of the hypothesis that the phosphorylation of elF-2 by these kinases is basis of the control of initiation in lysates incubated under these conditions.

TL;DR: The current data indicate that the coated regions of plasma membrane are specialized structures of rapid turnover that function to carry receptor-bound LDL, and perhaps other receptor- bound molecules, into the cell.

TL;DR: A very limited number of discrete rabbit DNA fragments was found which could form well matched hybrids with PβG1 DNA, consistent with the presence of a single copy of the β-globin gene per haploid genome.

TL;DR: The properties of the cornified envelopes (insolubility in detergent and reducing agents, and solubility following proteolytic digestion) are readily explained by a structure consisting of a cross-linked protein lattice.

TL;DR: Three aspects of terminal differentiation of the epidermal keratinocyte have been studied in cell culture—the development of detergent-insoluble cytoplasmic filaments, the formation of a cornified cell envelope and the destruction of the cell nucleus.

TL;DR: The results suggest an attachment of polyribosomes to the cytoskeleton via mRNA, which is similar to that in intact cells.

TL;DR: It is proposed that the protein functions during DNA replication by facilitating priming of the progeny strands, thus allowing the 5′ ends of the DNA to be replicated.

TL;DR: It is proposed that during glycosylation of asparagine residues, a common oligosaccharide is transferred from the lipid carrier to protein and is subsequently processed to yield the so-called "high mannose" and "complex" oligOSaccharides.

TL;DR: If the tracks of many cells are examined, cell line characteristic track patterns become apparent and second or third generation descendents of 3T3 cells were observed to repeat track patterns of their ancestor cell.

TL;DR: Most of the cell lines established from methylcholanthrene-induced fibrosarcomas of Japanese quail are susceptible to subgroup A avian sarcoma viruses, but are relatively resistant to viruses of subgroups C, E and F as compared to normal quail embryo fibroblasts.

TL;DR: The results suggest that LETS protein may have a role in cell attachment and spreading, and affect the organization of cytoskeleton.

TL;DR: The similarity in protein composition of core RNP particles from different cell types is consistent with a conserved particle structure and function in eucaryotes.

TL;DR: The faithful representation of β-globin mRNA in the PβG1 DNa insertion establishes the validity of using cloned DNA, initially derived from double-stranded DNA transcripts of mRNA, for studying the structure of eucaryotic genes.

TL;DR: Messenger RNA which stimilates the synthesis of myosin heavy chain in a reticulocyte lysate has been isolated from single myogenic cell cultures, and an active fraction from sucrose gradients migrates as 26S on formamide-polyacrylamide gels.

TL;DR: The insert appears to be a general property of the rabbit β-globin gene, even in tissues in which this gene is active, which suggests that the insert is not involved in inactivating the gene in nonerythroid tissues.

TL;DR: The polypeptides synthesized in E. coli minicells from recombinant plasmids containing DNA fragments from cauliflower mosaic virus, Drosophila melanogaster, and mouse mitochondria were examined and it is probable that the Eco RI site in pSC101 lies within the structural gene for apolypeptide involved in tetracycline resistance, and insertion of DNA into this site may also result in the synthesis of a truncated or elongated polype

TL;DR: Preliminary experiments suggest that even the "low uptake" form of α-l-iduronidase may be taken up by receptor binding, although with much lower affinity.

TL;DR: It is concluded that the parental origin of X m and X p marks them as different from one another, and possible causes for the failure of the expression of X p in the yolk sac endoderm and the tissue specificity of the effect are discussed.

TL;DR: A cell-free system is described which assembles chromatin from purified DNA in 1 hr under physiological incubation conditions, which converts SV40 DNA to a nucleoprotein which co-sediments with naturally occurring SV40 chromatin and which can be cleaved by micrococcal nuclease to a highly ordered pattern of DNA fragments resembling those from digestion of liver chromatin.

TL;DR: It is demonstrated that mitochondrial DNA molecules replicated in a short time interval are randomly selected for later rounds of replication, and that replication of mitochondrial DNA continues throughout the cell cycle in mouse L cells.

TL;DR: The results indicate that the rate-limiting transition occurs within 2 hr of the start of DNA synthesis, and suggests that the transition probability depends upon the continuous synthesis of a protein with a short half-life, or on some other unstable substance whose concentration is geared to the rate of translation.

TL;DR: The conditions of in vitro suspension cultures of murine B lymphocytes are therefore perfect to the extent that every B cell capable of growth will grow as a single clone.