Showing papers in "Cell in 1979"

TL;DR: It is concluded that SV40 infection or transformation of mouse cells stimulates the synthesis or enhances the stability of a 54K protein, which appears to be associated with SV40 T antigen in SV40-infected and -transformed cells, and is co-immunoprecipitated by hybridomas sera to SV40 large T antigen.

TL;DR: This co-transformation system should allow the introduction and stable integration of virtually any defined gene into cultured cells and is likely to represent a subpopulation of competent cells which are likely to integrate other unlinked genes at frequencies higher than the general population.

TL;DR: The discovery of the induction of a unique set of puffs by heat shock (Ritossa, 1962) has led the way to an analysis of gene function and structure in Drosophila that is, so far, unique.

TL;DR: Three new mesenchymal phenotypes were expressed in cultures of Swiss 3T3 and C3H/10T1/2CL8 mouse cells treated with 5-azacytidine or 5-aza-2'-deoxycytidine, implying that cell division was obligatory for the expression of the new phenotypes.

TL;DR: Both peptide mapping and immunochemical analysis indicated that the 130K protein is not related either structurally or antigenically to other mechanochemical proteins, including α-actinin, actin, myosin, tropomyos in, filamin and tubulin.

TL;DR: Late in the terminal differentiation of epidermis and cultured epidermal cells, a protein envelope located beneath the plasma membrane becomes cross-linked by cellular transglutaminase, and cannot be extracted from keratinocytes after their envelopes have become cross- linked.

TL;DR: All but one of the deletion mutants tested were defective for transformation of rat embryo and rat embryo brain cells, and no viral DNA synthesis was observed in mutant virus-infected HeLa cells.

TL;DR: Chicken hematopoietic cells transformed in vitro and in vivo by seven strains of replication-defective avian leukemia viruses were assayed for the expression of six erythroid and five myeloid differentiation parameters, including differentiation-specific surface antigens as detected by newly developed antisera.

TL;DR: It is demonstrated that the expression of many early adenovirus mRNAs is dependent upon the activity of a pre-early viral product, which is defective in adanovirus 5 host range (Ad hr) group I mutants.

TL;DR: Specific transcription initiation at the late promoter is observed with extracts derived from either virus-infected or uninfected KB cells and with class II RNA polymerases isolated from either human calf, murine or amphibian cells.

TL;DR: A model of reverse transcription has been devised by which the detailed architecture of ten molecular structures is predicted, and it appears to provide a credible description ofreverse transcription.

TL;DR: A model in which the nucleoprotein fiber is folded into loops which are arranged in the Chromatid in radial fashion, in such a way that their bases become the central axis of the chromatid is discussed.

TL;DR: The results suggest that the fibronectin and actin fibers of the fibronexus are closely associated (maximum separation distances of 8--22 nm), if not co-axial.

TL;DR: Polyoma T antigen immunoprecipitates contain a protein kinase-like activity which preferentially phosphorylates material of 50-60,000 daltons molecular weight, which behaves identically to phosphate and differently than phosphorylated serine, threonine, lysine and histidine.

TL;DR: The sites of transcription initiation and termination on a cloned fragment of ribosomal DNA from X. laevis, and the surrounding nucleotides are located, and a sequence is found upstream from the 5' end of the gene that has 90% homology to the sequence from nucleotide minus 125 to +4 in the initiation region.

TL;DR: In this paper, Dispersed repetitive DNA sequences from yeast (Saccharomyces cerevisiae) nuclear DNA have been isolated as molecular hybrids in lambdagt and showed marked alterations in the size of the restriction fragments containing these repetitive DNAs.

TL;DR: The hormonal regulation of casein gene expression appears to be an "all or none" process occurring only at the transcriptional or post-transcriptional levels, but may involve a coordinated response at several levels to permit the efficient expression of specialized differentiated functions.

TL;DR: The incubation of the E coli DNA binding protein HU with relaxed circular SV40 DNA in the presence of pure nicking-closing enzyme introduces up to 18 negative superhelical turns in the DNA molecules as measured by agarose gel electrophoresis.

TL;DR: The results reinforce the association of PA with tissue remodeling; show that the enzyme can be used as an indicator of cellular response to a wide range of hormones in both normal and malignant tissue; and suggest that observations of this type in organ culture may be of some value in predicting physiological responses in vivo.

TL;DR: Calculations based on the divergence of the two preproinsulin nucleotide and amino acid sequences indicate that these genes are the products of a recent duplication.

TL;DR: The clathrin coat is a labile structure that can be solubilized by nondenaturing treatments, and baskets can be reformed from the extracted material.

TL;DR: Genetic data suggest that Tn3 contains a third site (Gill et al., 1978), designated IRS (internal resolution site), whose absence results in the insertion of two complete copies of Tn2 as direct repeats into the recipient DNA, and that the IRS site is required for recombination and subsequent segregation of the direct repeats to leave a single copy of TN3.

TL;DR: Three types of variant restriction enzyme patterns of globin DNA fragments were detected in otherwise normal individuals and can be used to derive an approximate estimate of the total number of different DNA sequence variants in man.

TL;DR: Restriction enzyme cleavage of the fragments generated indicates that the preferential DNAase I cleavage sites in chromatin are position-specific, and this nuclease also cleaves at position- specific sites.

TL;DR: The resolving power of the system is demonstrated by the clear resolution of over 250 fragments of the Eco RI digest of E. coli DNA, which provides a two-dimensional separation of fragments when combined with length-dependent electrophoresis in agarose in the perpendicular direction.

TL;DR: It is suggested that at least two time-ordered processes are necessary to initiate hepatic growth fully: first, activation of Na+ flux systems by peptides similar or identical to EGF; and second, potentiation of these and subsequent cellular events by the combined action of insulin plus glucagon.

TL;DR: Recent work has focused on the enzymatic differences between extracts of interferon-treated and control cells reported by several investigators, and studies examining the stability of 2,5A in cell extracts are described, which may help to understand how the endoribonuclease functions in virus-infected interferons-treated cells.

TL;DR: The presence of LETS protein and GP-2 in basement membranes suggests that there are subtle interactions which are important in adhesion of epithelial cells to basement membranes.

TL;DR: Centrioles in growing mammalian cells are primarily ciliated in a part of G 1 during which the cells can arrest in suboptimal environmental conditions, suggesting certain centriole events may therefore be necessary before DNA synthesis can be initiated in 3T3 cells.