Showing papers in "Cell in 1983"

TL;DR: The RNA moieties of ribonuclease P purified from both E. coli and B. subtilis can cleave tRNA precursor molecules in buffers containing either 60 mM Mg2+ or 10 mM MG2+ plus 1 mM spermidine, and in vitro, the RNA and protein subunits from one species can complement sub units from the other species in reconstitution experiments.

TL;DR: This work proposes a new mechanism for meiotic recombination, in which events are initiated by double-strand breaks that are enlarged to double- Strand gaps, and postmeiotic segregation can result from heteroduplex DNA formed at the boundaries of the gap-repair region.

TL;DR: A regulatory linkage between the function of two oncogenes--c-myc and c-sis--the latter being the putative structural gene for PDGF is suggested, consistent with a model that a labile protein may regulate c- myc levels in these cells.

TL;DR: PMOV-psi- has a defect in the packaging of genomic RNA into virions but can provide in trans the products necessary for virion production, and can be used to produce helper-free stocks of natural or synthetic defective retroviruses.

TL;DR: The fate of the transferrin receptor during in vitro maturation of sheep reticulocytes has been followed using FITC- and 125I-labeled anti-transferrin-receptor antibodies and it can be shown that at 0 degree C or in phosphate-buffered saline the rate of vesicle release is less than that at 37 degrees C in culture medium.

TL;DR: Eggs of the sea urchin Lytechinus pictus and oocytes of the surf clam Spisula solidissima also contain proteins that only start to be made after fertilization and are destroyed at certain points in the cell division cycle, and it is proposed to call these proteins the cyclins.

TL;DR: Results suggest that direct binding of Sp 1 to sequences in the upstream promoter element is the mechanism by which this factor activates transcription by RNA polymerase II at the SV40 early promoter.

TL;DR: It is proposed that this tissue-specific enhancer contributes to the activation of somatically rearranged immunoglobulin variable region genes and possibly to abnormal expression of other genes (e.g. c-myc) that become translocated to its domain of influence.

TL;DR: The DNA sequences derived from the germ line JH-C mu region are found to be required for accurate and efficient transcription from a functionally rearranged VH promoter, and the Ig gene enhancer appears to act in a tissue-specific manner.

TL;DR: The findings raise the possibility that the amyloid plaques observed in transmissible, degenerative neurological diseases might consist of prions, a polymeric form of the scrapie prion.

TL;DR: Fractions purified from scrapie-infected hamster brain contain a unique protein, designated PrP, which is found to be directly proportional to the titer of the infectious prion.

TL;DR: To study the regulation of the human beta-interferon (beta-IFN) gene by poly(I)-poly(C), the expression of deletion mutants of the cloned gene introduced into mouse cells on a new bovine papilloma virus vector is analyzed.

TL;DR: The screened cDNA library for gene sequences that are regulated by platelet-derived growth factor (PDGF) in BALB/c-3T3 cells indicates that these sequences correspond to "early genes" which are not induced as a consequence of cell growth, but rather are directly regulated by PDGF.

TL;DR: The results indicate that differentiated mouse muscle nuclei can activate human muscle genes in the nuclei of a cell type in which they are not normally expressed, and that this activation occurs via the cytoplasm.

TL;DR: It is hypothesized that E was part of a duplicated region of over 250 nucleotides flanking the src gene in an ancestral RSV, and that differential deletion of one copy of E led to its positional difference in Pr-C and SR-A.

TL;DR: Evidence is presented that the Wx locus encodes a starch granule-bound 58 kd polypeptide that is synthesized in vitro as a 65 kd precursor and it is shown that a mutation caused by the controlling element Dissociation is attributable to an insertion of approximately 2.4 kb at the WX locus.

TL;DR: In this article, a 16-kb insert that encoded enzymatic activities for the light reaction as well as regulatory functions necessary for expression of the luminescence phenotype (Lux) was found in a clone library of hybrid plasmids containing DNA from the marine bacterium Vibrio fischeri.

TL;DR: The tubular vesicles are, it is proposed, an intermediate in recycling the receptor to the cell surface in rats infused with asialoglycoprotein for 60 min, and appear to transform into secondary lysosomes, wherein the ligand is degraded.

TL;DR: A revertant of one of the white locus mutants has been found to result from precise excision of the P element, restoring the wild-type DNA sequence.

TL;DR: An assay was developed whereby transcription of a cloned, rearranged kappa gene could be detected following its transfection into antibody-secreting mouse myeloma cells, suggesting that after rearrangement the kappa variable region promoter is activated by sequences more than 2.6 kb downstream of J kappa.

TL;DR: Addition of the Sp1 factor stimulated SV40 early promoter transcription 40-fold, and inhibited adenovirus major late promoter transcription by 40%.

TL;DR: Antibody treatment delayed the onset of pulmonary metastasis, indicating that plasminogen activator is required during early stages of the process.

TL;DR: There are at least three different fibronectin mRNAs in rat liver which differ in coding potential and are probably all encoded by a single gene, according to the sequence data and S1 nuclease mapping.

TL;DR: Nucleotide sequence analysis of the cloned DNAs has yielded a complete amino acid sequence for bovine rhodopsin and provided an intron-exon map of its gene.

TL;DR: It is suggested that, in the eight lines which have amplified NB-19-21, the amplification units are overlapping, but not identical, and that transposition of the common sequences may occur prior to amplification.

TL;DR: It is concluded that the MTV promoter region contains a "glucocorticoid response element" that can be separated from a second element essential for MTV transcription initiation, implying that receptor binding at these sites in vivo may mediate hormone responsiveness.

TL;DR: The structure of the translocated gene and the nature of its linkage to the immunoglobulin locus and the presence of two c-myc promoters and consequently two long leader sequences raise novel possibilities for the activation of an oncogene.

TL;DR: Five regions of MTV DNA that are bound specifically by purified receptor are mapped and compared, suggesting that receptor affinity for upstream and internal regions may differ by less than one order of magnitude.