Showing papers in "Cell in 1984"

TL;DR: This pulsed field gradient gel electrophoresis fractionates intact S. cerevisiae chromosomal DNA, producing a molecular karyotype that greatly facilitates the assignment of genes to yeast chromosomes.

TL;DR: By reducing the size of the transposed sequence, it is concluded that Pro-Lys- lys- Lys-Arg-L Lys-Val can act as a nuclear location signal and may represent a prototype of similar sequences in other nuclear proteins.

TL;DR: It is clear that detailed understanding of the mechanism of regulation of CAMP synthesis will soon be achieved from study of the interactions of purified components that have been reconstituted in lipid bilayers of defined composition.

TL;DR: The highly specific presence of a chromosomal breakpoint within bcr within Ph'-positive CML patients strongly suggests the involvement of bcr in this type of leukemia.

TL;DR: It is concluded that the maternal and paternal contributions to the embryonic genome in mammals are not equivalent and that a diploid genome derived from only one of the two parental sexes is incapable of supporting complete embryogenesis.

TL;DR: Transfection of simian COS cells with the human LDL receptor cDNA linked to the SV40 early promoter resulted in expression of functional cell surface receptors.

TL;DR: Given that T11 is the earliest T-lineage surface glycoprotein to appear in thymic ontogeny and is thus expressed before T3-Ti, the former may be involved in clonal expansion and/or differentiation during early development.

TL;DR: Data are presented for sequence-specific chromatin-loop organization in histone-depleted nuclei from Drosophila melanogaster Kc cells and a family of attachment sites related by hybridization to those of the hsp70 genes was discovered.

TL;DR: A murine retrovirus shuttle vector system for the efficient introduction of selectable and nonselectable DNA sequences into mammalian cells and recovery of the inserted sequences as molecular clones is developed.

TL;DR: This antigenic determinant differs from those previously described for the hemagglutinin and clearly demonstrates the ability of synthetic peptides to generate antibodies that interact with regions of the protein not immunogenic or generally accessible when the protein is the immunogen.

TL;DR: An altered human c-abl protein (P210) with associated tyrosine kinase activity in the K562 leukemia cell line is detected and may have important implications for a mechanism of activation of this oncogene.

TL;DR: Although it has no obvious effect on the early development of these mice, the constitutionally deregulated myc gene appears to act as a heritable, predisposing factor favoring the accelerated development of a tissue-specific adenocarcinoma.

TL;DR: The 50 amino acid mature TGF-α produced by expression of the appropriate coding sequence in E. coli binds to the epidermal growth factor receptor and induces the anchorage independence of normal mammalian cells in culture.

TL;DR: It is shown that this DNA homology (the homeo box) is due to a conserved protein-coding sequence present in these three pattern-formation genes and is reflected in a structural homology in their gene products.

TL;DR: New genetic and functional similarities between divergent organisms have been discovered, providing convincing evidence of the evolutionary conservation of strategies used in the targeting and translocation of polypeptides.

TL;DR: Two distinct nonoverlapping enhancer elements can be defined within the polyoma enhancer region, which provides a 3-fold higher enhancement of the alpha 2-collagen promoter than element B in mouse fibroblasts and in mouse embryonal carcinoma cells.

TL;DR: The T-DNA of the Ri plasmid from Agrobacterium rhizogenes is compatible with the regeneration of whole plants from genetically transformed roots and is transmitted through meiosis to the progeny of genetically transformed plants in carrot, tobacco, and morning glory.

TL;DR: Gene dosage effects suggest that KEX2 is the structural gene for the endopeptidase, and cloned DNA restores both enzymatic activity in vitro and the normal pattern of proteolytic processing and glycosylation of prepro-alpha-factor in vivo.

TL;DR: Results demonstrate that expression of the myc and rasKi proto-oncogenes is dependent upon the cellular growth state, and that growth control exhibits growth-factor-dependent, cell-cycle-timed oncogene expression.

TL;DR: A P-element derivative called Pc[ry], which carries the selectable marker gene rosy, but which acts like a nondefective, intact P element, is made, which suggests that a single polypeptide comprises the transposase.

TL;DR: Transport of the VSV-encoded glycoprotein between successive compartments of the Golgi has been reconstituted in a cell-free system and is measured, in a rapid and sensitive new assay, by the coupled incorporation of 3H-N-acetylglucosamine (GlcNAc).

TL;DR: Eighteen cDNAs, cloned from interferon-treated T98G neuroblastoma cells, correspond to seven different mRNAs induced up to 40-fold by interferons, and one codes for metallothionein II and another for a class I HLA.

TL;DR: A number of mink cell focus-forming (MCF) proviruses was molecularly cloned from mouse lymphoma DNA to detect common integration regions in other MuLV-induced lymphomas and variations in the molecular structure of the corresponding region (Pim-1) in other lymphomas were revealed.

TL;DR: DnaA protein recognizes in addition to oriC a number of specific sites: within or near the replication origins of pSC101, pBR322, and ColE1; within the regulatory regions of the dnaA and "X-protein" genes; and in IRL-Tn5.

TL;DR: Results imply that sequences within IVS1 actively participate in splicing, and it is proposed that the 5' end ofIVS1 is joined by a 2'-5' phosphodiester linkage to the A residue in the RNAase T1 oligonucleotide ACTCTCTCTG located 28-37 nucleotides upstream from the IVS 1 3' end.

TL;DR: Comme les antigenes de classe I, les molecules of classe II du MHC forment une famille multigenique fortement polymorphe avec une complexite de determinants antigeniques dont la variabilite peut en partie produite par un mecanisme de reparation par copie mal compris analogue a la conversion genique.

TL;DR: A mildly acidic compartment, distinct from endocytic vesicles, that is involved in the recycling of internalized components back to the cell surface is defined.

TL;DR: While the REP sequences do not appear to modulate differential gene expression within an operon, they can affect the expression of both upstream and downstream genes to a small extent, probably by affecting the rate of mRNA degradation.