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JournalISSN: 1420-682X

Cellular and Molecular Life Sciences 

Birkhäuser
About: Cellular and Molecular Life Sciences is an academic journal published by Birkhäuser. The journal publishes majorly in the area(s): Medicine & Biology. It has an ISSN identifier of 1420-682X. Over the lifetime, 32241 publications have been published receiving 865067 citations. The journal is also known as: Cell Mol Life Sci.


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Journal ArticleDOI
TL;DR: A 1-step silver-staining technique, requiring only 2 min to perform, is described for the differential staining of nucleolus organizer regions.
Abstract: A 1-step silver-staining technique, requiring only 2 min to perform, is described for the differential staining of nucleolus organizer regions. A protective colloidal developer is used to control the reduction of the silver.

2,812 citations

Journal ArticleDOI
TL;DR: This work has shown that for specific tasks the Hsp70 cycle is coupled to the action of other chaperones, such as Hsp90 and Hsp100, and this ATPase cycle is controlled by co-chaperones of the family of J-domain proteins, which target H Sp70s to their substrates, and by nucleotide exchange factors, which determine the lifetime of the HSp70-substrate complex.
Abstract: Hsp70 proteins are central components of the cellular network of molecular chaperones and folding catalysts. They assist a large variety of protein folding processes in the cell by transient association of their substrate binding domain with short hydrophobic peptide segments within their substrate proteins. The substrate binding and release cycle is driven by the switching of Hsp70 between the low-affinity ATP bound state and the high-affinity ADP bound state. Thus, ATP binding and hydrolysis are essential in vitro and in vivo for the chaperone activity of Hsp70 proteins. This ATPase cycle is controlled by co-chaperones of the family of J-domain proteins, which target Hsp70s to their substrates, and by nucleotide exchange factors, which determine the lifetime of the Hsp70-substrate complex. Additional co-chaperones fine-tune this chaperone cycle. For specific tasks the Hsp70 cycle is coupled to the action of other chaperones, such as Hsp90 and Hsp100.

2,564 citations

Journal ArticleDOI
TL;DR: In this article, shocks di temperatura possono indurre una variazione di "puffing pattern" in ghiandole salivari di Drosophila, which is perfettamente reversibili and rappresentano zone di intensa sintesi di RNA.
Abstract: Si e notato che shocks di temperatura possono indurre una variazione di «puffing pattern» in ghiandole salivari di Drosophila. Tali «puffing» sono perfettamente reversibili e rappresentano zone di intensa sintesi di RNA. Si e notato che DNP e Na salicilato portano a simili variazioni di «puffing pattern».

1,946 citations

Journal ArticleDOI
TL;DR: This review discusses the dual action of AOS during plant stress responses, which was first described in pathogenesis but has also recently been demonstrated during several abiotic stress responses.
Abstract: Adaptation to environmental changes is crucial for plant growth and survival. However, the molecular and biochemical mechanisms of adaptation are still poorly understood and the signaling pathways involved remain elusive. Active oxygen species (AOS) have been proposed as a central component of plant adaptation to both biotic and abiotic stresses. Under such conditions, AOS may play two very different roles: exacerbating damage or signaling the activation of defense responses. Such a dual function was first described in pathogenesis but has also recently been demonstrated during several abiotic stress responses. To allow for these different roles, cellular levels of AOS must be tightly controlled. The numerous AOS sources and a complex system of oxidant scavengers provide the flexibility necessary for these functions. This review discusses the dual action of AOS during plant stress responses.

1,815 citations

Journal ArticleDOI
TL;DR: A comprehensive overview of extracellular vesicles is given in this article, where the authors compare results from meta-analyses of published proteomic studies on membrane Vesicles.
Abstract: Release of membrane vesicles, a process conserved in both prokaryotes and eukaryotes, represents an evolutionary link, and suggests essential functions of a dynamic extracellular vesicular compartment (including exosomes, microparticles or microvesicles and apoptotic bodies). Compelling evidence supports the significance of this compartment in a broad range of physiological and pathological processes. However, classification of membrane vesicles, protocols of their isolation and detection, molecular details of vesicular release, clearance and biological functions are still under intense investigation. Here, we give a comprehensive overview of extracellular vesicles. After discussing the technical pitfalls and potential artifacts of the rapidly emerging field, we compare results from meta-analyses of published proteomic studies on membrane vesicles. We also summarize clinical implications of membrane vesicles. Lessons from this compartment challenge current paradigms concerning the mechanisms of intercellular communication and immune regulation. Furthermore, its clinical implementation may open new perspectives in translational medicine both in diagnostics and therapy.

1,737 citations

Performance
Metrics
No. of papers from the Journal in previous years
YearPapers
2023166
2022637
2021484
2020319
2019310
2018294