Showing papers in "Československá patologie in 1989"

Fluorescence microscopy detection of mycopathogens using Rylux BSU

Abstract: Rylux BSU (Synthesia, Pardubice--Semtin), a derivative of diaminostilbendisulphonic acid, has a good affinity to chitin of fungal cell walls as well as to cellulose of algae. A histologic method using this compound for detection of fungi in dermatology is presented accompanied with results of a quick diagnostic procedure. Pathogenic fungi were stained with a 2 per cent solution of Rylux BSU in phosphate buffer (pH 7.3) or in a 2 per cent aqueous solution of NaOH with an admixture of 10 per cent dimethylsulphoxide. A yellow green fluorescence of the cell walls of fungi (tested on 52 species) and algae (2 species) was evoked by incident blue light (max. filter transmission for 405 nm) combined with a barrier filter (max. 578 nm). Fluorescence was not fading and remained stable in Canada balsam. Moreover, the method could be applied on sections previously stained (HE, Weigert, Giemsa, KOH-preparations) because of more information offered by a combined procedure.

[Thin-needle aspiration cytology of the thyroid gland].

Abstract: A group of 187 fine needle aspiration cytology materials from thyroid gland was classified according to international cytology classification. The most important diagnostic criteria were presented for single lesions and correctness of cytologic evaluation compared with a complete bioptic finding.

Sebaceous skin tumors

Abstract: A group of 197 sebaceous tumours comprised mainly Jadassohn type nevi (96). Sebaceous adenoma (epithelioma) was as frequent as sebaceous carcinoma (24). Differential diagnosis distinguishing rare nosological items was discussed.

Epithelioid hemangioendothelioma of the liver

Abstract: Epitheloid hemangioendothelioma of the liver was found at autopsy of two women and one man (at the age of 67, 74 and 68). It occurred in multinodular form or as a huge tumours infiltration. In one case secondaries were noticed in a portal lymphnode and in the lung. Histology was characterized by epitheloid and elongated cells with irregular nuclei and sparse cytoplasmic vacuoles. A massive growth of tumour into sinusoid, central and portal veins resulted in a progressive liver atrophy or destruction. Differential diagnosis comprised epithelial tumours, especially cholangiocellular carcinoma, angiosarcoma and non-neoplastic processes (liver fibrosis and venoocclusive disease.

Quantitative ultrastructural characteristics of mitochondria and myofibrils in the myocardium in chronic hemodynamic overload

Abstract: Stereological morphometry was used to determine ultrastructural changes of the myocardium in the course of well defined chronic hemodynamic overload Overload was induced in the hearts of rabbits by perforation of aortic valves Relative volumes of mitochondria, myofibrils were measured in tissue samples taken from subendocardial and subepicardial region of the left ventricle In the course of developing hypertrophy relative volume of mitochondria significantly increased as well as volume ratio Mi/Mf, while those of myofibrils decreased Increased volume of mitochondria prolonged during the period of developed hypertrophy and its normalization occurred in the hypertrophy regression Failure of the heart was characterized by increased volume of mitochondria and decrease those of myofibrils Comparison of given morphometric data in subendocardium and subepicardium of the left ventricle did not find regional differences in subcellular composition of cardiomyocytes in the course of chronic hemodynamic overload