Showing papers in "Chemico-Biological Interactions in 1984"

TL;DR: The covalent binding characteristics of synthetic N-acetyl-p-benzoquinoneimine (NAPQI), the putative electrophilic intermediate produced during oxidative metabolism of acetaminophen, paralleled closely those of the reactive species generated metabolically.

TL;DR: The results can be seen as confirmation that alkylation of inorganic arsenic acts as a detoxification mechanism in mammals.

TL;DR: The results of flux measurements show that two mercury compounds cross the BLM but the overall permeabilities are dependent on the pH of the aqueous media, and are not apparently influenced by the different phospholipid constituents of the bilayers.

TL;DR: A comparison of the Sephadex LH-20 column profiles of purine adducts derived from [14C]benzene- and [3H]dGMP-labelled mtDNA with profiles generated by individually incubating each of the putative unlabelled metabolites with bone marrow mitoplasts in vitro has indicated that catechol is probably not a major metabolite of benzene formed in bone marrow cell mitochondria.

TL;DR: It is suggested, that the modulation of the extracellular activity ofcartilage-degrading enzymes by cartilage-derived factors may explain, at least in part, the beneficial effects of some therapeutically used chondroprotective agents.

TL;DR: The data confirm recent findings on hepatocyte heterogeneity in ammonia metabolism and point to a role of an impaired glutamine synthesis, but not to an impairment of urea synthesis, in the pathogenesis of hyperammonemia in acute severe liver disease like CCl4 poisoning.

TL;DR: The data indicate that TCB 3,4-oxide is a primary metabolic intermediate in the formation of methylthio-metabolites of TCB.

TL;DR: Wistar rats treated with lead nitrate were used in these experiments to provide evidence of the possible correlation between hyperplasia, induced cholesterol synthesis and the levels of glucose- 6-phosphate dehydrogenase (G-6-PD) in the liver.

TL;DR: Ethyleneimine was reacted with guanosine in aqueous medium and the products were purified by Sephadex G-10 and high-performance liquid chromatography (HPLC) and two products were identified by UV-spectroscopy: imidazole-ring opened 7-alkylguanosine and 1-alksylguanoine, accounting for 80% and 14% of all adduct radioactivity, respectively.

TL;DR: It is shown that Salmonella typhimurium strains TA98 and TA1538, which are sensitive to DNP and have wild type nitroreductase complements, do metabolize DNP to 1-amino-8-nitropyrene (ANP) and 1,8- diaminopyrene (DAP) but that these compounds are much weaker mutagens than DNP.

TL;DR: The results suggest that the in vivo administration of I-3-C may confer protection for hepatic macromolecules against covalent binding of the metabolites of these two indirect acting mutagens.

TL;DR: It is proposed that the entry of HNE into membranes lowers their glutathione GSH content and is associated with an increased lipid peroxidation measured in vitro which is blocked by added GSH or alpha-tocopherol.

TL;DR: The hypothesis that cadmium and mercury incorporation into rat hepatic metallothionein during the first few hours after exposure to these metals can occur primarily by displacement of zinc from preexisting zinc thionein by a process which does not require new protein synthesis is supported.

TL;DR: It is demonstrated that AN can directly alkylate DNA in vitro at a physiological pH and temperature and evidence is presented which strongly suggests that N6- CE-dAdo was formed by Dimroth rearrangement of 1-CE-d adducts during the reaction between AN and dAdo.

TL;DR: Results indicated that selenite catalyzed a reduction of O2 in glutathione dependent redox cycles with NADPH as an electron donor with indications that O2 reduction was terminated by selenites biotransformation to methylated metabolites.

TL;DR: In this article, the FAD-containing monooxygenase (FMO) has been purified from both mouse and pig liver microsomes by similar purification procedures, revealing significant differences in catalytic and immunological properties.

TL;DR: It is demonstrated that the 3-methylcholanthrene-inducible UDP-glucuronosyltransferase is preferentially expressed in centrilobular hepatocytes, suggesting that the differentially inducible enzyme activities may be localized in different zones of the liver lobule.

TL;DR: The in vivo formation of benzo[alpha]pyrene (BP) metabolite-DNA adducts in several tissues of mice and rabbits was examined, and the tissue-to-tissue variation in protein binding levels of BP metabolites was greater than that for BPDEI-DNAAdducts.

TL;DR: Based on both in vivo and in vitro data, the formation of this product appears to be mediated by cytochrome P-450 and to involve a reactive intermediate that may be responsible for the observed covalent binding of radiolabeled morphine to proteins and, at least in part, for the morphine-induced depletion of GSH in the rat.

TL;DR: The irreversible binding of chlorpromazine radical cation (CPZ+.) and photoactivated chlor Promazine ( CPZ) to calf thymus DNA in vitro and bacterial macromolecules in intact bacterium cells was investigated and the consequences of covalent binding for the cytotoxicity and genotoxicity of CPZ+.

TL;DR: Some basic characteristics of the enzyme system involved in the oxidative metabolism of xenobiotic compounds were investigated in Drosophila melanogaster flies and response to enzyme inducing compounds was found to be strain-dependent.

TL;DR: Results are compatible with the suggestion that 1-naphthol may exert its toxicity to isolated hepatocytes and other cellular systems by metabolism to naphthoquinones followed by their redox cycling with concomittant generation of active oxygen species in particular superoxide free radicals.

TL;DR: Evidence is provided that under standard conditions, human UDPGT activities are comparable to those from other animals.

TL;DR: The results indicate that fetal organs can metabolize those oncogenic compounds at an early stage of the development, and that the metabolic pathways and DNA adducts are quite similar to those in experimental animals in which the compounds are carcinogenic.

TL;DR: Five cytosolic glutathione transferases were isolated from the liver of the male little skate, Raja erinacea, a marine elasmobranch and purified to apparent homogeneity by this procedure, finding that bilirubin and hematin bound tightly to homogeneous E-4, with dissociation constants in the micromolar range.

TL;DR: The results suggest the involvement of lipid peroxidation, consequent to GSH depletion, in the pathogenesis of liver cell necrosis due to DBE.

TL;DR: DNA damage under an equivalent exposure situation was more pronounced in the salts/glucose than in the McCoy's medium and more striking in the latter medium than in alpha-MEM, suggesting irreversible interactions of HgCl2 with DNA may be responsible for its cytotoxic action in cells.

TL;DR: In this paper, it was shown that a relatively high dose of morphine rapidly results in depletion of hepatic glutathione (GSH) content and marked elevation of serum transaminase activity.

TL;DR: From data, it is evident that the neighboring bases affect the alkylation pattern of guanine by MNU, a finding that can have significant relevance in defining 'hot spots' ofAlkylation in the genome which may be important in the processes of mutagenesis and carcinogenesis.