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Showing papers in "Chemistry and Physics of Lipids in 1985"



Journal ArticleDOI
TL;DR: Experiments provide compelling evidence for a broad role of the FABPs in the transport, utilization and cellular economy of free fatty acids in the liver and small intestine, and also in protecting several aspects of cellular function against the modulatory effects of fatty acids, fatty acyl-CoA esters, and other ligands.

192 citations


Journal ArticleDOI
TL;DR: The results show that albumin in plasma transudates has the potential to seriously impair alveolar surfactant activity in vivo, but the detrimental effect will be mitigated if a critical threshold of phospholipid is present.

171 citations


Journal ArticleDOI
TL;DR: A lipid transfer protein was isolated from bovine liver and purified 75-fold to near homogeneity by a combination of DEAE-cellulose ion exchange, Sephadex G-200 gel permeation, and hydroxylapatite chromatography.

131 citations


Journal ArticleDOI
TL;DR: Gel formation and the typical morphology of the gels are discussed as arising mainly from strong intermolecular hydrogen bonds between amide linkages holding the molecules together and the influence of chiral centers of the carbohydrate chain which might be responsible for helical aggregates to be formed.

126 citations


Journal ArticleDOI
TL;DR: A binding protein is described for certain oxygenated derivatives of cholesterol which suppress 3-hydroxy-3-methylglutaryl coenzyme A reductase and cholesterol synthesis in cultured mammalian cells and it is proposed that the binding protein functions as a receptor for endogenous regulatory oxysterols.

116 citations


Journal ArticleDOI
TL;DR: The results described in the present review support the concept that intracellular lipid transfer is a highly specific process, far more substrate-specific than suggested by the earlier studies conducted using liposomal techniques.

107 citations


Journal ArticleDOI
TL;DR: The functional significance of the specific heart FABP is discussed in relation to myocardial fatty acid metabolism in normal and pathological conditions.

91 citations


Journal ArticleDOI
TL;DR: The transfer protein isolated from bovine brain transferred several different neutral glycosphingolipids and ganglioside GM1 equally well, but failed to accelerate phosphatidylcholine or sphingomyelin intervesicular movement.

68 citations


Journal ArticleDOI
TL;DR: The data show, that the combination of soft ionization mass spectrometry with classical EI-MS gives valuable information on the structure and homogeneity of gangliosides.

67 citations


Journal ArticleDOI
TL;DR: Recombinant DNA techniques combined with comparative sequence analyses offer a useful approach for defining unique as well as general structure-function relationships in this group of fatty acid binding proteins.

Journal ArticleDOI
TL;DR: In this paper, the bilayer to hexagonal phase transition of dioleoylphosphatidylethanolamine has been detected for the first time by differential scanning calorimetry.

Journal ArticleDOI
TL;DR: A new highly sensitive ligand-receptor binding assay and a new approach to biological signal amplifying based on the use of lipid-specific probes are described.

Journal ArticleDOI
TL;DR: Non-specific lipid transfer protein (nsL-TP) purified from rat and bovine liver accelerates the transfer of all common diacylglycerophospholipids, cholesterol as well as glycosphingolipid and gangliosides between membranes and is capable of mediating net transfer of cholesterol to membranes.

Journal ArticleDOI
TL;DR: In this article, the binding of CA2+ ions to bilayers composed of POPG alone or in mixture with other unsaturated lipids was studied with differential scanning calorimetry and deuterium and phosphorus-31 nuclear magnetic resonance (NMR).

Journal ArticleDOI
TL;DR: The phase equilibria in mixtures of dioleoylphosphatidylcholine (DOPC), DopC, and DOPE and water were studied by 31P-nMR and 2H-NMR as mentioned in this paper.

Journal ArticleDOI
TL;DR: The tissue distribution of PI-TPs and the means by which transfer activity is measured using natural and artificial membranes are examined and a role for PI- TPs is proposed.

Journal ArticleDOI
TL;DR: A comparison of the properties of both categories of proteins, PLTPs and FABPs, is presented and new perspectives opened by the use of these proteins as tools for membrane studies will be presented.

Journal ArticleDOI
TL;DR: The addition of fatty acids to racemic glycidol was catalysed by tributylamine to give 1-monoacylglycerols in high yield and purity.

Journal ArticleDOI
TL;DR: The hydrophobic region of the binding site of a bovine fatty acid binding protein (pI 7.0-FABP) has been characterized using fluorescence and circular dichroism (CD) spectroscopy and appears to be constructed for high affinity binding of long chain fatty acids.

Journal ArticleDOI
TL;DR: The specific ultrasonic absorption coefficient per wavelength as a function of temperature in the vicinity of the phase transition of liposomes, composed of a 4:1 mixture of dipalmitoylphosphatidylcholine andDPPC, was determined using an acoustic interferometer.

Journal ArticleDOI
TL;DR: Spectral properties of Merocyanine 540 were used in an attempt to investigate the interfacial micropolarity of liposomes constituted by egg phosphatidylcholine with phosphorus with results that reveal a more polar microenvironment for interfaces containing anionic glycosphingolipids or PA compared to interfaces constituted by PC alone or PC with neutral glycosphers.

Journal ArticleDOI
TL;DR: Fluorescent, non-radiolabeled analogues of phosphatidic acid were prepared by phospholipase D hydrolysis of fluorescent phosph atidylcholine by using dimethylaminopyridine as the catalyst.

Journal ArticleDOI
TL;DR: The structural and dynamic properties of aqueous dispersions of a cationic two-chain lipid containing one dienoyl group per chain for polymerization as well as of mixtures of this lipid with dimyristoylphosphatidylcholine (DMPC) were studied in this paper.

Journal ArticleDOI
TL;DR: From ESR spin labeling it is concluded that in sonicated dispersions the prodrug molecule is more mobile and its anisotropy of motion is reduced compared to unsonication dispersions.

Journal ArticleDOI
TL;DR: The tissue distribution of CRBP and CRABP, together with their relation to lipid transporting proteins suggests that CRBP or CRABP are cellular transporting proteins for retinol and retinoic acid, respectively.

Journal ArticleDOI
TL;DR: The first description of M1A type mycolates, and also of tri and tetraunsaturated mycolic acids, with both a total carbon number from 70 to 80 and a carbon number of the fatty acids from pyrolysis equal to 22 and 24 was given in this paper.

Journal ArticleDOI
TL;DR: Two activator proteins, GM2-activator and sphingolipid activator protein-1, are critically appraised on their glycolipid-binding properties and on their activity to facilitate the transfer ofglycolipids.

Journal ArticleDOI
TL;DR: The synthesis of phosphatidylcholines, PE and PS containing two acyl chains of the naturally occurring sponge fatty acid as well as its hitherto unknown geometrical isomers is described.

Journal ArticleDOI
TL;DR: The variations of the same parameters in vesicle preparations containing up to 15% of GM1 were still lower, indicating that the presence of ganglioside tends to increase the stability upon aging of small unilamellar vesicles.