scispace - formally typeset
Search or ask a question

Showing papers in "Chinese Journal of Biotechnology in 2008"


Journal ArticleDOI
TL;DR: In this paper, the role of key enzymes in microalgal fatty acid biosynthesis and problems in manipulation of lipid production in microalgae via genetic engineering, especially proposals and measures for exploitation and utilization of oil-bearing transgenic micro algae for biodiesel production are also discussed.

162 citations


Journal ArticleDOI
TL;DR: Development of large-scale culture methods using bioreactors has opened up feasibilities of production of secondary metabolites at the industrial levels and adoption of elicitation methods have shown improved synthesis ofsecondary metabolites in adventitious root cultures.

110 citations


Journal ArticleDOI
TL;DR: This fungal taxol also had strong anticancer activity against some cancer cells tested by Apoptotic assay and it is indicated that with the increase of taxol concentration from 0.005-0.05 micromol/L, taxol induced increased cell death through apoptosis.

64 citations


Journal ArticleDOI
TL;DR: From the estimated values of the Luedeking-Piret kinetic model parameters, alpha and beta, it was found that the lipase production by Candida rugosa is growth associated.

57 citations


Journal ArticleDOI
TL;DR: It is proposed that this most deleterious nsSNP with an SNPid rs28897759 is an important candidate for the cause of breast cancer by BRCA2 gene.

39 citations


Journal ArticleDOI
TL;DR: In the batch fermentation of curdlan, a relatively higher NH4Cl level with continuous glucose feeding increased the cell density leading to improvement ofCurdlan production and biomass production was not improved significantly, but both production and yield were enhanced remarkably.

29 citations


Journal ArticleDOI
Xingjiang Bu1, Xin-Jun Du1, Wenjie Zhou, Xiao-Fan Zhao1, Jin-Xing Wang1 
TL;DR: The results of semiquantity RT-PCR showed that FcLyz was constitutively expressed in tested tissues in a low level in normal shrimp, and up-regulated in hemocytes, heart, hepatopancreas and gill of bacterial challenged shrimp.

24 citations


Journal ArticleDOI
TL;DR: Progresses in biotransformation of the indigo produced by microorganisms will bring us a greener method of indigo and indigo-like pigments production.

24 citations


Journal ArticleDOI
TL;DR: A systematic method for constructing a large scale kinetic metabolic model and its initial application to the modeling of central metabolism of Methylobacterium extorquens AM1, a methylotrophic and environmental important bacterium is reported.

24 citations


Journal ArticleDOI
TL;DR: The structure-function relationship of ACE as well as its gene polymorphism and inhibitor development are reviewed, finding that the catalytic mechanisms of the two active sites of somatic ACE in the cleavage of angiotensin I and bradykin are different.

23 citations


Journal ArticleDOI
TL;DR: The enzymatic characteristics Km, k(cat), pH and temperature optima of the purified laccase have been determined using 2, 6-dimethoxyphenol as the substrate and have been found to be 35 micromol/L, 0.30 min(-1), 4.5 and 37 degrees C respectively.

Journal ArticleDOI
TL;DR: This study provides a basis for the further study on Radix Isatidis, a traditional Chinese medicinal (TCM) herb, and found that benzoic acid promoted the growth of Staphylococcus aureus and Shigella dysenteriae.

Journal ArticleDOI
Mingqian Hu1, Jiongkun Wang1, Jiye Cai1, Yangzhe Wu1, Xiaoping Wang1 
TL;DR: Atomic force microscope was used to study the biotoxicity of food preservative sodium benzoate at the single cellular level and the statistical analysis of lymphocytes after treatment with SB was studied.

Journal ArticleDOI
TL;DR: The SPAC reactor shows good performances in adapting the continuous change of influent COD and HRT, and thevolumetric COD removal rate and volumetric biogas production were increased by 191% and 195%, respectively.

Journal ArticleDOI
TL;DR: Optimization of property-improved biodiesel manufacturing process coupled with AB extractive fermentation was conducted, under the condition of using the biodiesel originated from waste cooking oil as the extractant and high concentrated corn flour medium to eliminate energy-consuming product recovery process.

Journal ArticleDOI
TL;DR: Polymerase chain reaction (PCR) analyses confirmed the hairy root lines and regenerated plants were induced by A. rhizogenes and displayed a relatively fast development procedure and stronger than the aseptic seedlings.

Journal ArticleDOI
TL;DR: Curtobacterium luteum, a gram-positive psychrotrophic bacterium, secreting an extracellular protease was isolated from the soil of Gangotri glacier, Western Himalaya and was stimulated by manganese ions; however, it was inactivated by copper ions.

Journal ArticleDOI
TL;DR: Concanavalin A is immobilized on a pre-activated chitosan microspheres, and then oriented immobilization of urease is carried out based on the strong interaction between ConA and glycoprotein, which presents higher temperature resistance, higher affinity to the substrate, and higher stability of operation.

Journal ArticleDOI
TL;DR: The background, the suggested definition, the theoretical framework and methodologies of this new discipline as well as its challenges and future development are discussed.

Journal ArticleDOI
TL;DR: This paper considers RNA structure prediction as a 2-classes classification problem: given a sequence alignment, to decide whether or not two columns of alignment form a base pair, and introduced a similarity weight factor, which could adjust the contribution of covariation and thermodynamic information toward prediction according to sequence similarity.

Journal ArticleDOI
TL;DR: The results suggest that beta-catenin plays an important role in the undifferentiated state maintenance of preadipocytes and the inhibition of porcine adipose tissue development, the mechanism maybe due to its regulation function on the adipogenic transcription factors PPARy, C/EBPalpha and early differentiation marker gene LPL.

Journal Article
TL;DR: Sequencing analysis showed that an open reading frame (ORF) of 2223 bp, termed umcel3G, potentially encodes a beta-glucosidase, confirming that this ORF encodes this enzyme has potential applications in the fermentative production of ethanol by simultaneous saccharification and cofermentation (SSCF) of lignocelluloses.

Journal Article
Keya Cai1, Keping Chen, Xiaoyong Liu, Qin Yao, Jun Li 
TL;DR: The exclusive expression of aminoacylase in highly resistant strain and near isogenic line and its absence in susceptible strain suggest that it might be a BmNPV-resistance related protein.

Journal ArticleDOI
TL;DR: An L-amino acid deaminase from Proteus mirabilis is expressed in E. coli in an active state and was found to be associated with membranes, likely to be necessary for its enzymatic activity.

Journal ArticleDOI
Yuan Zhou1, Xiaoting Huang1, Guihai Huang1, Xiaobo Bai1, Xianlai Tang1, Youzhi Li1 
TL;DR: ShakingBioleaching of Cu and Fe in low-grade chalcopyrite using Penicillium janthinellum strian GXCR was studied and was much better than submerged bioleaching.

Journal Article
TL;DR: It is conclued that hASCs obtained from human amniotic fluid could differentiate into cardiomyocytes through the formation of embryonic bodies.

Journal ArticleDOI
Rong-Yue Lei1, Yuhuan Qiao1, Jidong Yan1, Shuang Yang1, Tianhui Thu1 
TL;DR: The study alsodemonstrates that in E. coli BL21trxB(DE3) cytoplasm, which is a thioredoxin reductase mutant strain, soluble homodimeric BMP6 can be formed and the overexpressed MBP-BMP6 fusion protein is purified by chromatography, and shown to be functionally active.

Journal ArticleDOI
TL;DR: A strain of cellulase with high cellulase activity was screened from rice straw compost and classified as Penicillium decumbens by the analysis of its morphology and 18S rRNA gene sequences, which showed heat and alkali resistant characteristics.

Journal ArticleDOI
TL;DR: A revolutionary MDV-DNA engineering platform utilizing RED/ET recombination system was constructed successfully, which can help the understanding ofMDV gene functions and promote the using of MDV as a vector for expressing foreign genes.

Journal ArticleDOI
H Lu1
TL;DR: The results show that mhPI is capable of mediating high-level GK1 gene expression and has similar antibacterial activity against Gram-positive and Gram-negative bacteria.