Showing papers in "Chinese New Drugs Journal in 2002"

Classification and Development of TCM Fingerprint

Abstract: The TCM fingerprint is one of the strategic subjects for TCM modernization. It is not only a mode and technique for TCM quality control, but also a research mode and strategy for TCM theories and new drugs development. The TCM fingerprint need to have stable characteristics, reproducibility and operability. The development of TCM fingerprint consists of two stages: the primary stage and the superior stage. The target of the primary stage is to develop an strategy for establishing a fingerprint and evaluate the conformability. The target of the superior stage is to study the correlation between characteristic of TCM fingerprint and the pharmacodynamics, that is a research on the biological equivalence of TCM fingerprint.

Studies on chemical constituents in dried buds of Lonicera japonica Thunb.

Abstract: Objective:To research chemical constituents from the dried buds of Lonicera japonica Thunb.and to find out the active constituents of plant.Methods:The chemical constituents of the plant were identified by means of UV,IR,MS,JH1,D,Z NMR,JC13,D,Z NMR,respectively,in some cases by direct comparison with authentic samples.Study on anti bacterial activity was carried out.Results:Eleven compounds were obtained and identified as luteolin (Ⅰ),corymbosin(Ⅱ),5 hydroxyl 7,3′,4′ trimethoxy flavone(Ⅲ),5 hydroxyl 7,4′ dimethoxy flavone(Ⅳ),quercetin(Ⅴ),lonicerin (Ⅵ), quercetin 3 O β D glucoside(Ⅶ), 3 O [ α L rhamnopyranosyl(1→2) α L arabinopyranosyl] 28 O [ β D glucopyranosyl(1→6) β D glucopyranosyl] oleanolic acid (Ⅷ),chlorogenic acid (Ⅸ),daucosterol (Ⅹ),and sucrose (Ⅺ).Conclusion:Compound Ⅳ was first isolated from Genus Lonicera ,while compounds Ⅴ, Ⅵ, Ⅷ and Ⅹ were isolated from the plant for the first time.Some compounds show anti bacterial activities.

HPLC determination of mianserin in human plasma

Abstract: Objective:To establish a HPLC method for the determination of mianserin in human plasmaMethods:Chlorprothixene was used as the reference compoundThe extracts of samples were chromatographed on a hypersil ODS 2 C 18 reversed phase column after two steps liquid liquid extraction by Diethyl ether absolute and monitored by UV detection at 240nmThe mobile phase consisted of CH 3OH∶H 2O∶ammonium acetate (containing of ammonium acetate and ammonia solution, 005 mol·L -1 and 01mol·L -1 respectively) (90∶7∶3,pH=77) at a flow rate of 1mL·min -1 Results:The calibration curve was linear over the range of 5～140μg·L -1 ( r =09990)The intra day RSD was less than 55%, and the inter day RSD was less than 88%The recovery was more than 90% and the limit of quantificaiton was 5μg·L -1 Conclusion:The method has good selectivity, sensitivity and reproducibility and can be used for pharmacokinetic studies of mianserin in humans and the clinical determination of mianserin

Antitumor activity and immunopharmacological effects of Linglong liquidum extractum (LLE)

Abstract: Objective:To investigate the antitumor activity of Linglong liquidum extractum (LLE),a Chinese folk medicine prescription, on transplanted tumor from animal and from human lung cancer cells in mice, and the antitumor effect of LLE on HL 60 tumor cell line in vitro ,and observe the effects of LLE on immune functions in mice bearing tumor.Methods:Antitumor activities were assayed by weighing tumor weight and the survival period of mice bearing tumor.MTT method was used to observe the T lymphocytes proliferation.Differences in ear weight challenged by DNFB were utilized for DTH response.TNF α activities were measured by crystal violet staining assay using L 929 cells.Results:LLE at dosage of 13.1,17.5 and 35.0g·kg -1 (corresponding to raw material) prolonged the survival time of mice bearing EAC.LLE at the dose as above had inhibition rate of 47.7%,66.1%, 82.0% for S 180 tumor and 67.0%,73.3%,76.4% for H 22 tumor,respectively.Growth of HL 60 tumor cell line was markedly inhibited by LLE in vitro .LLE at the dose of 17.5 and 35.0 g·kg -1 could inhibit the growth of lung adenocarcinoma cells in nude mice in vivo by a inhibition rate of 60.5% and 77.8%, respectively.The DTH responses were enhanced after oral administration of LLE.The T lymphocytes proliferation of mice bearing S 180 tumor could be increased by LLE.The production of TNF α from the supernatant of peritoneal macrophages in mice bearing tumor was enhanced.Conclusion:LLE has obvious antitumor effects and immunological enhancing effects.

Nanoparticulate systems for drug delivery

Abstract: Nanoparticulate systems are highlighted as potential drug delivery systems for both chemical drugs and biological macromolecular drugs in pharmaceutical area as the nanotechnology is developing rapidly in academic and industrial field. The main kinds of nano-delivery, methods of nanoparticles preparation and surface modification, function and mechanism, such as improved solubility,increased absorption and targetability, are discussed.

Bactericidal action of 5 kinds of traditional herbal drugs for Helicobacter Pylori

Abstract: Objective:To study the bactericidal action of 5 kinds of traditional herbal drugs for Helicobacter pylori (Hp).Methods:The 5 kinds of traditional herbal drugs,namely coptis chinensis ,the fruit of trifolite orange, Lithospermum erythrorhizo Sieb.et Zucc.and two decoctions of herb medicine,were tested for bactericidal action for Hp by the method of plate smearing with the concentration of 40,50 and 55mg·mL -1 ,separately.Results:In the scale of the selected concentration,the 5 kind of traditional herbal drugs had the bactericidal action for Hp,which became stronger with rise of its concentration.Conclusion:The coptis chinensis ,the fruit of trifolite orange and one decoction of herb medicine had notable effect,the coptis chinensis had most notable effect, the sterilizing rate was 99.8% with its concentration of 50mg·mL -1 . [

Hepatoprotective and choleretic action of Picrorhiza scrophulariaeflora Pennell

Abstract: Objective:To study the hepatoprotective and choleretic action of Picrorhiza scrophulariaeflora Pennell through animal experiments.Methods:The effect of Picrorhiza scrophulariaeflora Pennell on biochemical indexes in the model animals and normal animals was detected by biochemical methods.Results: ① The ethanol extract of the rhizome of Picrorhiza scrophulariaeflora Pennell significantly lowered the elevated level of serum ALT and AST in acute liver damage mice induced by carbon tetrachloride(CCl 4),thioacetamide(TAA) or acetaminophen(APAP).② It restrained the elevation of serum ALT and AST and increased total protein in subacute liver damage rats induced by CCl 4.③ It increased biliary secrection in normal rats.Conclusion:The ethanol extract of the rhizome of Picrorhiza scrophulariaeflora Pennell has the hepatoprotective and choleretic action. [

Synthesis of celecoxib

Abstract: Objective:To study the synthetic process of celecoxib,a selective cyclooxygenase 2 inhibitorMethods:Celecoxib was synthesized through Claisen condensation and cyclization from the starting compounds p methylacetophenone,ethyl trifluoroacetate and 4 hydrazinobenzene sulfonamide hydrochlorideResults:In the Claisen condensation procedure,ether was used, and the sequence of adding material was changedAfter cyclization,the crude product was recrystallized from EtOAc n hexane or purified by flash chromatography on silica gelThe overall yield was 54%( recrystallization) and 59%( flash chromatography),respectivelyConclusion:This synthesis process has some advantages,such as easier operation,shorter react time and higher yield

Itopride hydrochloride,a new gastrointestinal prokinetic agent

Abstract: Itopride hydrochloride( itopride ) is a new gastrointestinal prokinetic agent.It regulated motility of the gastrointestinal tract through synergism of its dopamine D 2 receptor antagonistic action and its acetylcholine esterase inhibitory action.The concentrations of itopride in the brain were fairly low while it was distributed in the body.No evidence was found for its serious adverse reaction including its proarrhythmic effect.With data obtained from series of clinical study,it suggested that itopride could be a new choice of drug for treatment of gastroinestinal symptoms caused by reduced gastrointestinal motility in functional dyspepsia and chronic gastritis. [

Efficacies of Sanjiuweitai-based quadruple regimens for the treatment of peptic ulcer disease and eradication of Helicobacter pylori

Abstract: Objective:To compare the efficacies of Sanjiuweitai and colloidal bismuth subcitrate based quadruple regimens for the treatment of peptic ulcer disease and eradication of Helicobacter pylori (Hp).Methods:102 patients with Hp associated peptic ulcer were randomly divided into three groups,group A( n =48) received Sanjiuweitai based quadruple regimen (Sanjiuweitai 2.5g,bid+ranitidine 150mg,bid+amoxicillin 500mg,bid+metronidazole 400mg,bid); group B 1( n =31) received colloidal bismuth subcitrate based quadruple regimen[colloidal bismuth subcitrate (Livzon) 110mg, bid+ ranitidine 150mg,bid+amoxicillin 500mg,bid+metronidazole 400mg,bid];group B 2( n =23) received ranitidine based triple regimen(ranitidine 150mg,bid+amoxicillin 500mg,bid+metronidazole 400mg,bid). Antibiotics were given for two weeks,the other drugs were given for six weeks, the efficacies were determined by gastroendoscopy or JC13,D,Z UBT.Results:The ulcer healing rate were 90.9%,100%,86.4% in group A,B 1,B 2 respectively; the stage S 2 achievement rate were 68.2%,73.1% and 45.5%; the eradication rate of Hp were 76.1%, 73.3% and 52.6%,the symptoms were remissive similarly in three groups,there were no side effects in three groups.Conclusion:Sanjiuweitai based quadruple regimen was similar to colloidal bismuth subcitrate based quadruple regimen in the ulcer healing and Hp eradication rate. [

Clinical evaluation for the efficacy and safety of polyethylene glycol 4000 in the treatment of functional constipation

Abstract: Objective:To evaluate the efficacy and safety of polyethylene glycol(PEG) 4000 in the treatment of functional constipation in comparison with lactulose.Methods:A total of 186 patients with functional constipation were enrolled in this multi center,randomized,comparative trial.They received PEG 4000(10g,bid, n =92) or lactulose(15mL,bid, n =94) for 2～4weeks.Results:The complete remission of constipation was reported by significantly higher number of patients treated with PEG 4000 in comparison with lactulose group (2 weeks: 70.7% vs 50%, P 0.01;4 weeks: 83.6% vs 57.1%, P 0.01).The mean Bristol score number of stool consistency during the 4 weeks treatment was higher in the PEG group(4.02±0.34 vs 3.58±0.16, P 0.01).At the end of 4 weeks study,PEG normalized the stool consistency in 82.6% patients(lactulose group: 60.0%, P 0.01 ),thus, PEG 4000 was more effective in producing “nonhard” stool than lactulose.There was no severe adverse effect in both groups.It is also likely that PEG 4000 is the most acceptable.Conclusion: PEG 4000 is an effective and safe agent in the treatment of functional constipation in adults. [

Effects of chlorpromazine and clozapine on the cognitive function in first-episode schizophrenia patients

Abstract: Objective:To approach the changes of cognitive function for first episode schizophrenia with antipsychotic drugs treatmentMethods:Seventy eight first episode schizophrenic patients were treated with chlorpromazine and clozapine randomly and double blindCognitive function of both groups were assessed with Wisconsin Card Sorting Test (WCST),WAIS R,WMS and Language Fluency Test etc before and twelve weeks after treatment Results:Extensive cognitive function impairment were observed in firist episode schizophreniaThe performance of WCST,Language scores of WAIS R,Picture and comprehension scores of WMS,Language Fluency Test were significantly improved in clozapine group than that in chlorpromazine group at the end of treatmentThe performance of WCST was obviously correlated with SANS,Tardive dyskinesia and GAF,but it had no correlation with Electroencephalogram normal or abnormalThe changes of cognitive function in patients had some differences between chlorpromazine and clozapine groupsConclusion:Clozapine can improve significantly cognitive function of schizophrenia,and chlorpromazine has no obvious roleThe changes of cognitive function were not of equal rank improvement of psychotic symptoms

Molecular characteristics and clinical application prospect of abrin

Abstract: Abrin is one of the potent toxins isolated from the beans of plant Abrus precatorius L.The toxic protein consists of two disulfide bonded subunits,the A and B chain.The B chain is a galactose specific lectin that facilitates the binding of abrin to the cell membrane and the endocytosis of A chain.After entering the cells,the A chain catalytically inactivates 60S ribosomal subunits by removing adenine position 4324 of 28S r RNA,and thereby inhibits protein biosynthesis.The structural characteristics,toxicological mechanism and clinical application prospect of abrin were reviewed in this paper.

Research Advances in Human Cytochrome P450s and their Application in New Drug Evaluation

Abstract: Cytochrome P450s (CYP450s) involved in the phase I metabolism of a variety of chemically diverse substances ranging from endogenous compounds to xenobiotics Up to date,55 sequenced CYP genes and 29 pseudogenes were found in the human In western countries CYP450s are estimated during new drug screening and safety evaluation In this study, we established the methods for assaying the CYP450s in different animal liver microsomes to accelerate the discovery of new drugs Based on the established methods, the effects of leflunomide,rh-IL11 and rh-EGF on CYP450s were evaluated The results showed that no significant changes of the protein concentration and the total contents of cytochrome P450s in beagle dogs, rats and rhesus monkeys were observed at different sampling timeThe specific activities of the CYP1A1, CYP2A6, CYP2C8/9 and CYP2E1 in beagle dogs were not changed after administration leflunomideIn rats, rh-IL11 had obvious inhibitory effect on the activities of the CYP1A1 and CYP2A2 but had no effect on the activities of the CYP2E1The specific activities of the CYP1A1, CYP2A6, CYP2C8/9, CYP2C19, CYP2D6 and CYP2E1 in rhesus monkeys were not changed after administration rh-EGF The methods established for assaying cytochrome P450s activities in dogs, rats and monkeys liver microsomes in these studies were reproducible, simple and rapid, useful in early drug screening and safety evluation For the new biotechnology derived drugs, we also should emphasize the importance of estimating the effects on CYP450s

Effect of dihydroartemisinin on the proliferation of MCF-7 cell

Abstract: Objective:To study the effect of dihydroartemisinin, a possible precursor in artemisinin biosynthesis,on the proliferation of MCF 7 cell.Methods:Cytotoxity assay was tested by SRB method on human breast tumor cell MCF 7.Cell cycle was determined by using flow cytometry(FCM) analysis, apoptosis was valued by both FCM and DAPI stain.Results:Dihydroartemisinin effectively inhibited the proliferation of MCF 7,the IC 50 was 0.26μmol·L -1 .The FCM data showed that the distribution of MCF 7 cells among S phase was reduced greatly when treated for 24h with 1μmol·L -1 dihydrateartemisinin, apoptosis could be observed both by FCM and DAPI stain.Conclusion:Dihydroartemisinin could significantly inhibit the proliferation of MCF 7 cell and affect cell cycle.

Pharmacological action and clinical application of secnidazole

Abstract: Secnidazole is a new drug of nitroimidazole family.Its pharmacology properties are similar with other nitroimidazoles,such as metronidazole and tinidazole.In this paper,the mechanism of action,pharmacology,pharmacokinetics properties,toxicity and clinical application of secnidazole were reviewed. Just like other nitromidazole drugs,secnidazole has the antimicrobial activity.It can be absorbed orally,and its biological availability is (100±26)%.With secnidazole at a single 0.5～2g dose orally, C max is 35.7～46.3mg·L -1 ,the T max is 1.42～3h, t 1/2 β is 17～29h. The safety and tolerability of secnidazole is good.The LD 50 of secnidazole is 2.5g·kg -1 .In clinical research,secnidazole single dose treatments are widely used in the treatment of amoebiasis,giardiasis,trichomoniasis and genitourinary infections.

Recent advances in immunosuppressive drugs

Abstract: Immunosuppressive drugs take more and more importantrole in organ transplant ation.During recent years,the success ratio of organ transplant ation has been more improved by the using of powerful and lower toxicity immunosuppressive drugs.The mechanism,clinical uses and adverse reactions of immunosuppressive drugs which have been used or studied in clinic were reviewed,including mycophenolate mofetil,tacrolimus,sirolimus,mizoribine,humanized monoclonal antibody,FTY720 etc.

Recent pharmacology progress in flupirtine, a new analgesic agent

Abstract: Flupirtine,2 amino 3 ethoxycarbonylamino 6 (p fluorobenzylamino) pyridine is a novel non opiate centrally acting analgesic agent without adverse reactions and physical dependence on prolonged administration.Flupirtine has been clinically used in relieving moderate acute pain of various types,for example,surgery and traumatic injury.In addition,flupirtine is also a useful agent for the treatment of chronic painful conditions such as the pain due to malignancy.In comparison with opiate drugs, flupirtine exerts its analgesic effects through different mechanisms of action because flupirtine requires an intact noradrenergic system to produce its analgesic effect. It has been found that antagonizing NMDA receptor is one of the analgesic molecular mechanisms.

Studies on preparation and in vitro-release characteristics of a sustained-release avermectins-gelatin microspheres

Abstract: Objective:To prepare a sustained release avermectins gelatin microspheres(AVM GMS) and study its in vitro release characteristics.Methods:AVM GMS was prepared from the biodegradable gelatin by emulsion polymerization,and the optimum formulation and preparation technical process was afforded by the uniform test.The release profile of AVM GMS was shown from the accumulative drug release amount in vitro .Results:The experiment conditions were optimized, the mean volume diameter obtained was 10～200μm and encapsulation rate was 68.6%～74.1%.The profile could be described by Higuchi equation.The release t 1/2 of AVM GMS was about 6.6 times as much as that of original AVM.Conclusion:AVM GMS had obviously sustained effect. [

A double-blind and double-dummy comparative study of quetiapine and chlorpromazine in the treatment of schizophrenia

Abstract: Objective:To evaluate the efficacy and safety of quetiapine for treatment of schizophrenia.Methods:221 cases of schizophrenic patients were randomly divided into quetiapine group( n =114) and chlorpromazine group( n =107), and treated for eight weeks.The PANSS was adopted for evaluating therapeutic effects.Results:The total effective rates in quetiapine and chlorpromazine group were simillar,with no significant difference.Less adverse reactions were found in quetiapine group.Conclusion:The domestic quetiapine is an effective and safe agent for treatment of schizophrenia.

Edaravone,an ethical brain protecting agent for acute cerebral infarction

Abstract: Edaravone is a newly developed free radical scavenger with hydroxyl radical scavenging activity.Recently,edaravone has been found to display potent neuroprotective effects in various models of excitotoxic cell damage, global and focal ischemia.The pharmacological action,pharmacokinetics,clinical evaluation,side effects and current opinion of edaravone, an ethical brain protecting agent for acute cerebral infarction,are reviewed in this paper.

Protective effect of bicyclol on acetaminophen induced liver mitochondria injury in mice

Abstract: Objective:To study the protective effect of bicyclol on acetaminophen (AP) induced liver mitochondria injury in mice.Methods:Serum aminotransferase ( ALT and AST) level and liver tissue damage were determined for evaluating the effect of bicyclol on AP induced liver injury in mice.The AST and GSH(glutathione) content in mitochondria,membrane swelling and membrane fluidity of mitochondria as well as the damage in mitochondria ultrastructure were also determined for evaluating the effect of bicyclol on AP induced mouse liver mithchondria injury.Results:Oral administration of bicyclol 50 and 150 mg·kg -1 decreased serum ALT and AST level and ameliorated liver tissue damage induced by AP.Bicyclol also prevented the decrease of AST and GSH content and the injury of mitochondrial ultrastructure,increased the membrane fluidity and sensitivity of membrane swelling to the exotic Ca 2+ stimulation.Furthermore,bicyclol added in vitro could also inhibited mitochondrial membrane swelling induced by TritonX 100 and high concentration of Ca 2+ stimulation.Conclusion:Bicyclol has protective action against injury of liver mitochondria induced by AP in mice.

The efficacy of Laolining capsule in treatment of 70 patients of acute and chronic diarrhea

Abstract: Objective:To observe the safety and efficacy of Laolining (LLN) capsule in treatment of acute and chronic diarrhea.Methods:A multi center,randomized,double blind,double dummy and controlled trials were adopted in the studies.140 patients were divided to receive twice daily LLN capsule of 1.26g,or twice daily Miya tablet of 1.05g for 3～7d or 14～21d.Results:For treatment of acute and chronic diarrhea,the effective rate of LLN capsule was 98.0%(40/41)and 100%(29/29),and Miya tablet was 98.0%(40/41),86.0%(25/29),respectively in studies.No significant differences were observed between LLN capsule and the control drug (Miya tablet) in studies.Conclusion:LLN capsule is an effective agent in treatment of acute and chronic diarrhea, the safety and efficacy of LLN capsule are similar to that of Miya tablet.

Capsaicin receptor,a novel target for new peripheral acting analgesics

Abstract: The capsaicin (vanilloid) receptor is a cation channel expressed by primary sensory neurons of the "pain" pathway, which accounts for the pain sensation of nociceptive stimulation.In laboratory research,the mice showed pain behavior after the activation of capsaicin receptor.The capsaicin receptor null mice were severely deficient in their responses to the noxious stimuli of capsaicin,acid,or heat(43℃). With the research furthering,more attention has been paid to the capsaicin receptor 1(VR1) as a novel target for analgesics.\;

Anti-diarrhea effect of Laolining capsule in mice with experimental diarrhea

Abstract: Objective:To study anti diarrhea effect of Laolining (LLN) capsule in mice with experimental diarrheaMethods:According to the methods of Xiong's diarrhea model of animal caused by antibiotics,150 BALB/C of mice were given orally 015g of twice daily and randomly equally divided into 5 groups,2 groups for LLN capsule of 10 7cfu·mL -1 and 10 6cfu·mL -1 ,05mL, po ,bid,2 groups for Miya tablet of 10 7cfu·mL -1 and 10 6cfu·mL -1 ,05mL, po ,bid,and one group for normal saline 05 mL for 3dThe changes of becterial group and defecation were observed after treatmentResults:The experimental diarrhea was occurred in all miceAt the second day the antidiarrheic rate in LLN capsule(10 7 cfu·mL -1 ) groupMiya tablet (10 7cfu·mL -1 ) group and normal saline group was 870%, 80% and 50% respectively,and Clostridium butyricum was colonizated in interstinal tractThe viable bacteria amounts of Bifidobacterium and Llactis of two treatment groups were significantly increased in comparison with normal saline group ( P 001) Conclusion:LLN capsule has obvious anti diarrhea effect in mice with experimental diarrhea

Studies on pharmacokinetics and relative bioavailability of dipyridamole sustained release pellets

Abstract: Objective:To study the relative bioavailability and bioequivalence of dipyridamole(DIP) sustained release pellets in Beagle dogsMethods:A rapid and sensitive RP HPLC assay was modified for the determination of the drug levels in plasmaIn a randomized two way crossover design,50mg single dose po DIP common tablets and sustained release pellets,respectively,were given to 4 male Beagle dogsThe parameters were estimated by two compartment model with a statistic analysis of ANOVAResults:The T max , C max , AUC 0～12 , t 1/2 α, t 1/2 β and MRT of DIP common tablets and sustained release pellets were(092±009)h and(157±012)h,(4344±3693)ng·mL -1 and (27952± 248)ng·mL -1 ,(92256±5273)ng·h·mL -1 and (1 15287±5518)ng·h·mL -1 , (069± 005)h and (089±012)h,(471±071)h and (176±553)h,(291±014)h and (354± 006)h,respectivelyThe mean relative bioavailability of the DIP sustained release pellets vs common tablets was (12506±438)%Conclusion:The results suggested that the DIP sustained release pellets were more excellent in bioavailability and absorption than common tablets

The advances in research of the biodegradation and biocompatibility of PLA and PLGA microspheres

Abstract: The biodegradation of PLA(poly-lactic acid) and PLGA(poly-lactyl coglycolid) mincrospheres is a homogeneous process and be influenced by relative molecular weight and its distribution,and size of the microspheres.The tissue response to biodegradable microspheres injected may be characterized by three phases.The biodegradation and biocompatibility of PLA and PLGA microspheres were reviewed by the author.

In vitro screening and assays for anti-HIV agents

Abstract: The appropriate screening models and methods in vitro or in vivo are very important in drug discovery.Because there is no ideal AIDS animal model available currently,the preclinical evaluation of anti HIV agents in vitro is the cornerstone of drug development. This review has outlined briefly the anti HIV testing strategy.

The analysis of effectiveness and cost of lamivudine in treatment of chronic hepatitis

Abstract: Objective:To analyze the effect,side effects and cost of lamivudine in treatment of chronic hepatitis.Methods:102 patients with chronic hepatitis whose values of ALT≥120IU·L -1 were divided into lamivudine group( n =34),interferon group( n =33) and the group treated with the protect liver and lower aminotransferase drugs.The patients treated for more than 6 months were detected the serum HBV DNA and HBeAg as anti viral parameter,ALT and the improvement of clinical symptom as effective parameter,and the comparison of drug cost effectiveness as pharmaco economic parameter.Results:There were no significant differences in ALT and the improvement of clinical symptom between lamivudine group and the other two,the effective rate were 88.24%,78.79%, 82.86% respectively.The HBV DNA negative turning rate in lamivudine group was obviouly bigger than those in the other two,the rate were 88.2%,39.4%,11.4% respectively.The HBeAg negative turning rate in lamivudine group was similar to that in interferon group(26.5% vs 24.2%),and was significantly bIgger than that in the group treated with the protect liver and lower aminotrausferase drugs(11.4%).Interferon group had more side effects(25/33) than the other two.The medical expenditure was that interferon groupthe group treated with the protect liver and lower aminotransferase drugslamivudine group.Conclusion:Lamivadine is effective and economical in treatment of chronic hepatitis.

HPLC determination of aucubin in compound Salvia plebeia granules

Abstract: Objective:To establish a HPLC method for the determination of aucubin in compound Salvia plebeia granules.Methods:Aucubin was determined on KF C 18 colum(250mm× 4.6 mm,10μm) with mobile phase of methanol water phosphoric acid(30∶969.5∶0.5) and the temperature at 35℃.The detective wavelength was 203nm and the flow rate was 1.0mL·min -1 .Results:The linear range of aucubin was 0.05～0.80μg,the mean average recovery was 84.46% with the RSD of 1.37% ( n =9).Conclusion:The method is simple,sensitive and highly reproducible and may be used for quality control of the granules.