scispace - formally typeset
Search or ask a question
JournalISSN: 0009-5893

Chromatographia 

Springer Science+Business Media
About: Chromatographia is an academic journal published by Springer Science+Business Media. The journal publishes majorly in the area(s): High-performance liquid chromatography & Reversed-phase chromatography. It has an ISSN identifier of 0009-5893. Over the lifetime, 10904 publications have been published receiving 165188 citations. The journal is also known as: Chromatographia (Berlin) & Chromatographia (Wiesbaden. Print).


Papers
More filters
Journal ArticleDOI
TL;DR: In this paper, it was shown that the use of the McGowans characteristic volume, Vx, is equivalent to using Leahy's computer-calculated intrinsic volumes, V1, for the cavity term mV/100, for 209 gaseous, liquid, and solid solutes.
Abstract: In the correlation of reversed-phase liquid chromatography capacity factors through the equation, $$\log k' = log k'_0 + mV/100 + s\pi _2^* + b\beta _2 + a\alpha _2 $$ the use of McGowans characteristic volume, Vx, which can be trivially calculated, is entirely equivalent to the use of Leahy's computer-calculated intrinsic volumes, V1, for the cavity term mV/100. It is shown that for 209 gaseous, liquid, and solid solutes, the two sets of volumes are related through the equation, $$V_1 = 0.597 + 0.6823 V_x $$ with a standard deviation of only 1.24cm3 mol−1, and a correlation coefficient of 0.9988.

765 citations

Journal ArticleDOI
TL;DR: Electrochromatographic methods can provide a wide range of partitioning methods of extremely high plate efficiency, and will, in future, undoubtedly rival conventional HPLC.
Abstract: In electrochromatography, electrolyte is driven along a narrow bore chromatographic column at typical HPLC linear velocities by applying a potential gradient of around 50,000 V m−1. Contrasting with pressure driven LC, the tube bore is limited to not more than 200 µm by self heating. Thus miniaturisation is mandatory in electrochromatography whereas it is optional in pressure driven LC unless very small particles (e.g. 1 µm diameter) are used. Because the velocity profile in open tubular electrochromatography is close to that of perfect plug flow, contrasting with the parabolic flow profile in pressure driven LC, open tube electrochromatography provides plate efficiencies limited only by axial diffusion, and comparable to those obtainable in open tubular gas chromatography. Contrasting with pressure driven open tubular LC there is no requirement for the tube bore to be very small (e.g. <10 µm). Although pure electrophoresis is applicable only to ionised solutes, and is not strictly a chromatographic method, neutral species can be chromatographed by partitioning them between two phases which move at different rates, for example by using micellar solutions or colloidal sols as the moving fluid, thereby retaining the high plate efficiency of electrophoresis. With packed tubes electrochromatographic separations of high efficiency can be obtained with 5 µm particles and extremely high efficiencies should be obtainable without sacrifice of eluent velocity by using submicron particles. In principle, electrochromatographic methods can provide a wide range of partitioning methods of extremely high plate efficiency, and will, in future, undoubtedly rival conventional HPLC.

495 citations

Journal ArticleDOI
TL;DR: In this paper, the authors review test procedures and recommend standards for a) the experimental and test parameters which must be recorded in order to enable comparisons to be made from laboratory to laboratory, b) the group of chromatographic parameters which best represent column performance for comparative purposes, with methods for their calculation, c) test solutes and eluents for some different types of packing materials.
Abstract: Comparisons of columns, column packings and column packing methods are made difficult and sometimes invalidated by differences and inadequacies in the test procedures used and the experimental data recorded. This paper reviews test procedures and recommends standards for a) the experimental and test parameters which must be recorded in order to enable comparisons to be made from laboratory to laboratory, b) the group of chromatographic parameters which best represent column performance for comparative purposes, with methods for their calculation, c) test solutes and eluents for some different types of packing materials. A computer program in BASIC is given which converts the experimental parameters into relevant chromatographic parameters.

478 citations

Journal ArticleDOI
TL;DR: It is shown that, following theory, there is no evidence of any reduction in electroosmotic velocity in columns packed with particles down to 1.5 μm diameter, and that reduced plate heights as low as unity are attainable for unretained solutes.
Abstract: Electrochromatography (that is HPLC where the eluent is driven along the column by electro-osmosis using fields of up to 100 kV m−1) promises plate efficiencies for HPLC which are comparable to those attained in capillary gas chromatography, but this requires that narrow-bore columns can be successfully packed with submicron particles. This paper demonstrates that we have now moved a considerable distance towards this goal. We show (1) that, following theory, there is no evidence of any reduction in electroosmotic velocity in columns packed with particles down to 1.5 μm diameter, (2) that reduced plate heights as low as unity are attainable for unretained solutes using both slurrypacked and drawn-packed columns 30 to 200 μm bore and up to 1 m long when packed with conventional 3 and 5 μm silica gels or with 1.5 μm impermeable silica spheres, (3) that columns driven electrically show higher plate efficiencies than identical columns driven by pressure, and (4) that 100,000 plate HPLC separations can be achieved in relatively short times of 30 minutes using in situ derivatised drawn packed capillaries containing 3 and 5 μm ODS-silica gels.

408 citations

Performance
Metrics
No. of papers from the Journal in previous years
YearPapers
202340
2022118
2021111
2020137
2019154
2018158