Showing papers in "Clinica Chimica Acta in 1980"

TL;DR: A modified assay for the determination of hydroxyproline in tissue is presented, which greatly reduce the time required for analysis of excised tissue as first introduced by Stegemann and Stalder.

TL;DR: The sensitivity of the colorimetric reaction and stability of the colour are enhanced over existing related procedures and the serum blank diminished, enabling urea to be precisely measured in micro amounts (1--5 microliters) of serum.

TL;DR: An improved method was improved for the separate determination of L-ascorbic acid and L-dehydroascorbIC acid in blood plasma, using dithiothreitol, to determine the content of ascorbic acid.

TL;DR: The method was evaluated by determining the efficiency of recovery of radiolabeled steroid glucuronides, and selected reference neutral free steroids of varied structure and polarity gave results equal to or better than those obtained by solvent or Amberlite XAD-2 extraction methods.

TL;DR: A new starch derivative for the determination of amylase activity has been synthesized by coupling Procion Yellow dye with starch, which is intensely yellow and is easily suspended in water, in neutral buffer solution, and also in acidic solution.

TL;DR: Experiments in which partially purified human erythrocyte PST was added to platelet, kidney and gut homogenates under these assay conditions provided evidence that endogenous PST inhibitors did not affect the observed enzyme activity.

TL;DR: A new enzyme-kinetic method based on radioimmunoassay of angiotensin I generated during incubation of plasma and excess sheep or ox renin substrate that does not require prior extraction of renin, is technically simple, and is sufficiently sensitive to measure subnormal renin levels.

TL;DR: The present case represents a newly described form of genetically determined myopathy, which is observed in an 18-year-old male who complained of exertional pigmenturia and easy fatigue and a marked increase in serum creatine kinase and myoglobinuria.

TL;DR: Statistical analysis of the data indicates that at least three different amino acid transport systems are operative in erythrocytes: a system for anionic amino acids (aspartate and glutamate), which concentrates these amino acids intracellularly, reaching high ERYthrocyte/plasma (E/P) concentration ratios; a concentrating system of A type, transporting serine, glycine and alanine and maintaining E/P ratios less than two; and a system of L

TL;DR: The results show that blood samples for serum zinc measurement should be taken in the morning in fasting state and the urinary zinc excretion rate should be related to the creatinine excretion rates to avoid sex differences.

TL;DR: The standard radioactive triolein emulsion was hydrolyzed by adipose tissue LPL at a rate closely similar to rat VLDL-triglyceride labeled in vivo with [1-14C]palmitic acid, suggesting that the experimental substrate behaved in a similar manner to the natural substrate.

TL;DR: The varied responses of folate mono- and polyglutamates in the radioassay procedures make this technique unsuitable for the determination of the mixture of folates derivatives that are normally encountered in biological extracts.

TL;DR: Serum sialic acid has been assayed enzymatically using the peroxidase-p-chlorophenol-4-amino-antipyrine method and showed good correlation between a chemical method and good reproducibility.

TL;DR: Analytic ultracentrifugation of serum lipoproteins from 80 men and 54 women used to determine if specific segments of the high density, low density and very low density lipoprotein schlieren curves could be defined so as to reveal significant correlations among them suggested that decreased HDL represents reduced HDL2-9 and may be accompanied by increased LDL0-7 and VLDL.

TL;DR: The isoenzyme profiles of N- acetyl -β- d -glucosaminidase (NAG) were determined using semi-automated DEAE-cellulose chromatography and showed potential in the further characterisation and diagnosis of disease.

TL;DR: A reliable radioimmunoassay for human pancreatic secretory trypsin inhibitor (PSTI) has been developed and elevated values were observed in patients with acute pancreatitis, and in some patients with chronic relapsing pancreatitis.

TL;DR: The findings suggest that alterations in pteridine metabolism are common in malignant disease and the pathogenic, diagnostic and therapeutic significance of these changes remains to be established.

TL;DR: Hormonal therapy induced a rapid rise in the plasma copper concentrations and a concomitant marginal fall in zinc levels of the carcinoma patients, and the magnitude of these hormone-induced changes were dependent on the type of therapy prescribed to the patient.

TL;DR: Examiniation of purified heart, kidney, liver and spleen ferritin showed that the assay was highly specific for acidic isoferritins, suggesting a radioimmunoassay for acidic ferrtin purified from HeLa cells may be of value in the diagnosis of malignancy.

TL;DR: In a comparative study, seven different methods for the determination of cortisol in human plasma were evaluated, using routine patient samples, and it was found that all other methods correlated well with this assay.

TL;DR: The present report constitutes a sensitive method for determination of vitamin D and its main metabolites in 2 ml of human serum by omitting vitamin D itself, 25-OHD, 24,25-(OH)2D, 25,26-(OH]2D and 1,25)-OH2D could be determined in less than 0.5 ml serum.

TL;DR: The present method, suitable for routine purposes, provides a basis for external quality control of urinary cortisol estimations, which is inadequate with the non-specific methods usually applied.

TL;DR: Findings obtained in pathological material from 62 patients with the various forms of Niemann-Pick disease and 21 obligate heterozygotes by one or another method are reported.

TL;DR: Specific estimation of urinary cortisol by radioimmunoassay requires a preceding chromatographic technique of high efficiency, such as HPLC, which represents an adequate tool for the routine laboratory.

TL;DR: Circulating plasma levels of testosterone, luteinising hormone and follicle stimulating hormone were determined in adult Nigerian males and results are suggestive of an association of secondary hypogonadism with haemoglobin S disease.

TL;DR: A reliable method for the determination of uric acid in plasma or serum is described and the hydrogen peroxidase developed in the uricase reaction is used for the coupling of sulphonated dichlorophenol and 4-aminoantipyine to a red dye.

TL;DR: Two variants of a radioimmunoassay for the bipyridylium herbicide Paraquat are described and results from rapid clinical assay agree well with the existing colorimetric method.

TL;DR: Under the procedural conditions recommended for the assay the methods for the determination of lysosomal glycohydrolases in plasma appeared to be simple, sensitive and reproducible.

TL;DR: The main problems associated with gas chromatographic amino acid analysis were overcome by application of a novel approach called enantiomer-labelling: the optical antipode to each L-amino acid is added to the sample prior to the clean-up and serves as an internal standard having identical chemical properties.