Showing papers in "Clinica Chimica Acta in 1990"

TL;DR: A review of the human alkaline phosphatases dealing specifically with the gene loci, characterization and discrimination of the various enzymes, and polymorphism at the enzyme level.

TL;DR: The chemistry and molecular biology of transferrin is discussed, with special reference to the triple role of the acidification of endocytotic vesicles and the biochemical mechanisms involved in iron uptake.

TL;DR: The crystal structure of alkaline phosphatase from Escherichia coli, which is a prototype for mammalian APs, has been refined to a crystallographic R-factor of 0.184 at 2.0 A resolution, and the active site of the molecule is highly conserved but specific changes in the secondary ligands to bound phosphate and the Mg metal are observed.

TL;DR: A second incubation in the presence of excess beta-galactosidase is needed to avoid underestimation of galactose-6-sulphate sulphatase activity.

TL;DR: Serum concentrations of BC among persons aged 20-49 years were higher with increasing age in females, but not in males, while ZX concentrations rose in the age group of 20 to 59 years for both sexes and serum TO values in both sexes rose with age and were similar.

TL;DR: The correlation between cotinine concentrations in a single specimen of urine and in serum collected on the same occasion from 279 male smokers was significantly increased by adjusting the urinarycotinine levels for urinary creatinine concentration to take account of variations in urinary dilution between people.

TL;DR: Changes in the plasma concentration of several non essential amino acids are already present in the early stage of renal failure in patients with no sign of protein malnutrition: these may result from altered metabolic pathways of amino acids related to uremia and/or nephron loss per se.

TL;DR: An automated spectrophotometric technique for the assay of this sugar in urine is presented in which lactulose is linked by a series of enzyme reactions to the equimolar production of NADPH, representing a significant improvement in time, simplicity and precision on existing methods of analysis.

TL;DR: It is concluded that the enzyme aldehyde oxidase is the principal enzyme responsible for the formation of oxipurinol in man.

TL;DR: It was confirmed that Cu/Zn SOD levels in erythrocytes and blood plasma were significantly enhanced in patients with Down syndrome and was present at high concentrations in brain, adrenal gland, liver, heart and kidney.

TL;DR: An assay for human liver NNMT was developed based on the extraction of the positively charged reaction product into 60% isoamyl alcohol in toluene in the presence of the ion-pairing reagent 1-heptanesulfonic acid, and the product of the reaction was identified as N1-methylnicotinamide (NMN) by high performance liquid chromatography.

TL;DR: An increased requirement for dietary antioxidant with high intakes of fish oil concentrates in man is indicated, especially in young men aged 21-35 years.

TL;DR: A one-step sandwich enzyme immunoassay for soluble TM was developed by using 2 antibodies; one of them, which inhibited thrombin-binding to TM, was fixed to polystyrene balls, and the other, which did not inhibit the throm bin-binding, but inhibited the protein C-activating cofactor activity of TM was used as peroxidase-labeled conjugate.

TL;DR: A simple method for detecting an A----G substitution at cDNA nt 1226 by performing polymerase chain reaction (PCR) using a 5'-primer mismatched at one nucleotide so as to create an Xho I restriction site is described.

TL;DR: There was a shift in peak values for salivary cortisol and cortisone towards late morning: this may be due to a delay in the daily activation of the hypothalamic-pituitary-adrenal axis in these patients.

TL;DR: A monoclonal antibody has been used in a sensitive radioimmunoassay that measures 1,25-dihydroxyvitamin D2 (ercalcitriol) and 1, 25-dhydroxy vitamin D3 (calcitriols) with equal potency, which has particular application in patients treated with vitamin D2 since it can now be measured accurately in the presence of 1-25- dihydroxv vitamin D3.

TL;DR: Various types of collagen (I, III, IV, V, V) were identified in normal and varicose human saphenous veins using pepsin digestion and cyanogen bromide digestion followed by SDS-polyacrylamide gel electrophoresis.

TL;DR: An enzyme linked immunosorbent assay (ELISA) for the detection of neuron specific enolase (NSE) in cerebrospinal fluid (CSF) was developed and was used to measure CSF-NSE levels in patients with neurological disorders to establish its potential usefulness and clinical application.

TL;DR: It was concluded that the effect of THM on CaOx crystallization in urine depends upon the methodology used to assess it and that promotion would only be expected in vivo in cases of extreme dehydration.

TL;DR: It is shown that on hospital admission, all serum assays were equally sensitive, but on subsequent days lipase, trypsinogen and elastase-1 assays all significantly surpassed the sensitivity of the serum amylase assay, and on the second and subsequent hospitalization days, determination of timed urine amylases excretion offered no advantage over the serumAmylase, and the ratio of amyl enzyme and creatinine clearances lacked discrimination altogether.

TL;DR: Methods have been developed for labeling DNA probes with acridinium esters at high specific activity and for stabilizing the label under the relatively harsh conditions of hybridization reactions, allowing a homogeneous assay format which does not require physical separation of hybridized and unhybridized probe.

TL;DR: Patients with coeliac disease should avoid ingestion of beer and foods that contain malt since they contain quantifiable amounts of hordein that is known to exacerbate the condition.

TL;DR: It is found that results from direct reading glucose electrodes must differ from the conventionally measured glucose concentration, which was observed in 159 whole blood samples which gave higher results from adirect reading glucose electrode than by the conventional method.

TL;DR: Control ranges have been established for plasma and urine carnitine concentrations in healthy children and adults, and for the carnitines content of liver and muscle in adults and no significant difference was found between fasting and post-prandial plasma carnite levels.

TL;DR: Measurement of the soluble extract-derived aminopeptidases described in this paper in urine may lead to an improvement upon existing assay procedures for early detection of kidney damage.

TL;DR: The clinical and biochemical features of five cases of alcaptonuria were reported and the concentration of homogentisic acid was determined in urine and also in plasma using a rapid, sensitive and specific HPLC method.

TL;DR: In this paper, a review of immunoassay and DNA hybridization methodologies is presented, with a focus on systems that introduce linear or exponential amplification, and the authors stress strategies, general principles and future trends.