Comparative Haematology International 

About: Comparative Haematology International is an academic journal. The journal publishes majorly in the area(s): Population & Mean corpuscular volume. It has an ISSN identifier of 0938-7714. Over the lifetime, 3151 publications have been published receiving 18689 citations.

The dextran sulphate sodium (DSS) model of colitis: an overview

Abstract: The dextran sulphate sodium model of colitis has demonstrated several correlations with human inflammatory bowel disease and is deemed suitable for investigating pathogenesis, therapeutic options and the dysplasia–adenocarcinoma sequence of inflammatory bowel disease. It is widely applicable to mice, rats, hamsters and guinea pigs. This review explores the features of this model and identifies areas for further research studies.

An automated biochemical assay for haptoglobin: Prevention of interference from albumin

Abstract: Measurement of the acute phase serum protein, haptoglobin (Hp), is performed by biochemical methods based on haemoglobin binding, in many veterinary diagnostic laboratories. During attempts to develop a robust biochemical assay for serum Hp it was discovered that serum albumin interfered with the assay system increasing results by as much as 0.28 mg/ml, which could affect interpretation of results especially in species with low normal Hp concentrations. A reagent cocktail (SB-7) was devised which inhibited the interfering effect of albumin. An automated assay for Hp utilising SB-7 was developed for production as a biochemical assay kit and was evaluated for use in veterinary diagnosis. The intra-assay coefficients of variation were of 0.9%, 0.9% and 1.3% for Hp concentrations of 2.0, 1.0 and 0.23 mg/ml, respectively and interassay coefficients of variation of 1.7% and 4.5% for Hp of 2.08 mg/ml and 0.24 mg/ml, respectively. The lower limit of detection of was 0.02 mg/ml, linearity extended to 8 mg/ml and recovery was 101±7% (mean ±SD). The assay had correlation coefficients (R2) of 0.96 and 0.90 when compared with immunodiffusion assays of canine Hp and bovine Hp, respectively. Lipaemia and bilirubinaemia caused no interference. Haemolysis did not affect measurement of low levels of Hp, but at serum Hp concentrations of 0.4 and 1.8 mg/ml the apparent Hp concentration was decreased. Elevated concentrations of Hp were measured in cattle with mastitis, dogs with polyarthritis and rats experimentally infected withBordetella pertussis. The automated assay is precise and has negligible interference from albumin.

Haematology and biochemical parameters of different feeding behaviour of teleost fishes from Vellar estuary, India

Abstract: Haematological parameters have been recognized as valuable tools for monitoring fish health. Haematological and serum biochemical parameters were studied and compared different feeding behaviour of teleost fishes. Three marine teleost fishes, Lates calcarifer (carnivores), Mugil cephalus (omnivores) and Chanos chanos (herbivores), were carried out in order to find out a normal range of blood parameters which would serve as baseline data for assessment of the health status of the fish as well as reference point for future comparative surveys. Blood parameters such as red blood cell count (RBC) and white blood cells count (WBC), haemoglobin, haematocrit, mean cell haemoglobin concentration (MCHC), mean cell volume (MCV), mean cell haemoglobin, glucose, protein, cholesterol and urea were estimated from teleost fishes of different trophic level. Statistical analysis revealed that differences in haematological parameters between marine fish were significant (P < 0.01). The result revealed that haematological RBC/WBC ratio, MCV and MCHC were significantly correlated at P < 0.05 level. The RBC/WBC level increased due to the decrease in WBC during the study. Blood serum biochemical parameters can be used for confirming the maturity and monitoring any changes in the quality of waters and related soils.

The influences of thermal stress on serum biochemical parameters of Iranian fat-tailed sheep and their correlation with triiodothyronine (T 3 ), thyroxine (T 4 ) and cortisol concentrations

Abstract: In order to study the influences of thermal stress on serum biochemical parameters of Iranian fat-tailed sheep and their correlation with triiodothyronine (T3), thyroxine (T4) and cortisol concentrations, an experiment was conducted using 45 clinically healthy Iranian fat-tailed sheep. These animals were divided into three experimental groups: group I, 15 sheep at 4 °C, group II 15 sheep at 21 °C and group III 15 sheep at 40 °C. Blood samples were taken from the jugular vein of all animals after 7 days’ exposure to the appropriate environmental temperature. The concentrations of total protein, glucose, cholesterol, total lipid, calcium, inorganic phosphorus, magnesium, creatine kinase, lactate dehydrogenase, triiodothyronine and thyroxine in cold stress conditions were higher than in heat stress; in contrast, the activities of aspartate aminotransferase and alanine aminotransferase were higher in heat stress conditions than in cold (p = <0.05). Although there were no significant differences in the concentrations of blood urea nitrogen, triglyceride, alkaline phosphatase and cortisol at either heat stress or cold stress, the first three assays were significantly lower than in animals kept at optimum temperatures, with cortisol having significantly higher values. These results revealed that very hot and very cold conditions had a profound effect on serum biochemical parameters.

Reference values of oxidative stress parameters (MDA, SOD, CAT) in dogs and cats

Abstract: The present study was performed on 56 clinically healthy dogs and 52 clinically healthy cats, age and sex matched. The reference values of principal parameters of oxidative stress—malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT)—were determined in blood samples of dogs and cats. It was found that MDA plasma concentrations in male dogs were higher than those in females. The same difference was found in cats but the sex difference was much higher. SOD activity did not show any sex differences in dogs or cats. The CAT values were lower in males compared to females in only cats .