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Showing papers in "Cryobiology in 2006"


Journal ArticleDOI
TL;DR: Whereas both AFP classes bind irreversibly to ice, the hyperactive AFPs are better at preventing ice growth out of the basal planes, and it is argued that this one consistent distinction in the behaviour of these two classes of AFPs is the key to hyperactivity.

254 citations


Journal ArticleDOI
TL;DR: This work examined the effect of a PVS2 cryoprotection protocol on the water content and phase behavior of mint and garlic shoot tips using differential scanning calorimetry and quantified the extent that ethylene glycol and dimethyl sulfoxide components permeate into shoot tips and replace some of the water.

128 citations


Journal ArticleDOI
TL;DR: It is concluded that new protocols and media are required for freezing hESC and safe storage at -80 degrees C as well as studies of the mechanisms of stress-related events associated with cell cryopreservation.

120 citations


Journal ArticleDOI
TL;DR: The multi-sample freezing device presented in this paper, the ice nucleus is produced in a separate volume of pure water inside an electrode cap, which means that the nucleus formation becomes independent of the sample composition, and allows automated freezing of many samples under equal conditions.

117 citations


Journal ArticleDOI
TL;DR: Three categories of freeze responsive genes have been identified from recent screenings of liver and heart from freeze-exposed hatchling painted turtles, suggesting that these defenses for freeze tolerance are aimed at counteracting the injurious effects of the ischemia imposed by plasma freezing.

115 citations


Journal ArticleDOI
TL;DR: Results showed that live cells of strain 34H incorporated substantial amounts of [3H]-leucine into TCA-precipitable material (primarily protein) down to -20 degrees C.

111 citations


Journal ArticleDOI
TL;DR: Using 31P solid-state NMR spectroscopy, it was determined that membrane fluidity increased concurrently with rapid cold-hardening of fat body cells, suggesting that membrane characteristics may be modified very rapidly to protect cells against cold-shock injury.

107 citations


Journal ArticleDOI
TL;DR: This review considers the current status of cryopreservation of human hepatocytes discussing the different stages involved in the process, including pre-treatment of cells, freezing solution, cryoprotectants and freezing and thawing protocols.

100 citations


Journal ArticleDOI
TL;DR: The objective of this study was to determine the viscosity of the residual unfrozen solution that cells are exposed to during freezing in the presence of glycerol and use this to interpret some key aspects of cryopreservation.

99 citations


Journal ArticleDOI
TL;DR: Data support an overall decrease in transcriptional activity in skeletal muscle of hibernating animals that is accomplished by multiple molecular mechanisms.

93 citations


Journal ArticleDOI
TL;DR: Wild-born cheetahs produce high proportions of pleiomorphic spermatozoa but with a high proportion of intact acrosomes, providing a user-friendly method to capture and store gametes to enhance genetic management.

Journal ArticleDOI
TL;DR: Comparison of these results with other published data for different drying conditions suggests that survival during storage is strongly influenced by the degree of vacuum under which the ampoules were sealed.

Journal ArticleDOI
TL;DR: A co-dependency between freezing rate and formulation ingredients is found, indicating a complex system and the need to use statistical tools to detect important interactions.

Journal ArticleDOI
TL;DR: Cells porated by this method and allowed an overnight recovery period exhibited improved dehydration tolerance suggesting a role for ATP poration in the anhydrous preservation of cells.

Journal ArticleDOI
TL;DR: A 'liquidus-tracking' method is developed that completely avoids the crystallization of ice and does not require rapid warming, and the ability of cartilage preserved in this way to incorporate sulphate into newly synthesized glycosaminoglycans (GAGs) approached 70% of that of fresh control cartilage.

Journal ArticleDOI
TL;DR: The attenuation of ice crystallization by syAFGP improves islet survival and function following cryopreservation and thawing.

Journal ArticleDOI
TL;DR: When plant cryoprotectants are characterized on a toxicological and biophysical basis, less damaging cryoprotsectant solutions could be developed.

Journal ArticleDOI
TL;DR: Whether cryoablated tumor draining lymph nodes (CTDLN) as adoptive immunotherapy may be an effective immunotherapeutic approach in the adjuvant treatment of breast cancer is examined.

Journal ArticleDOI
TL;DR: Protocols were developed to assess sperm cell membrane integrity and mitochondrial function by flow cytometry and to assess viability of eggs by fluorescence microscopy and cytotoxicity of cryoprotectant solutions and post-thaw damage to oyster eggs were assessed.

Journal ArticleDOI
TL;DR: In this article, the authors explored possible roles of autoxidation rate and the antioxidant enzymes associated with cryoprotective mechanisms in the plant cells and found that the reduction of cell viability with freezing activates a combination of antioxidant enzymes that results in intact cells.

Journal ArticleDOI
TL;DR: The ability to routinely cryopreserve micro-algal species reduces costs associated with maintaining large culture collections and reduces the risks of losing particular strains or species through contamination and genetic drift.

Journal ArticleDOI
TL;DR: Overall, antioxidant defenses appear to be part of the adaptive machinery for reptilian tolerance to sub-zero temperatures, and this may be in anticipation of the overgeneration of oxyradicals when the temperature increases.

Journal ArticleDOI
TL;DR: It is proposed that the nucleation and preferential growth of ice within the chondron (rather than the surrounding acellular matrix) is responsible for the very poor survival of chondrocytes in situ when current methods of cartilage cryopreservation are used.

Journal ArticleDOI
TL;DR: It is demonstrated that a small amount of chelating agent in the freeze-drying solution is necessary to prevent the deterioration of spermatozoa during freeze-Drying and subsequent preservation at 4 degrees C, and suggested that mouse spermutozoa can be frozen-dried in a simple solution containing the same composition as that used to preserve extracted DNA.

Journal ArticleDOI
TL;DR: Results suggest that to understand how carbohydrates relate to freezing tolerance, regions of the crown that endure freezing stress differently should be compared.

Journal ArticleDOI
TL;DR: The thermoperiodic acclimations resulted in the highest egg survival rates in both the short and the long period acclimation groups in comparison with the groups treated with constant temperatures.

Journal ArticleDOI
TL;DR: Investigation of the effect of sperm freezing medium dilution ratio, two cryoprotectants, and the addition of foetal bovine serum on the cryopreservation of European eel sperm found dilution ratios lower than 1:5 (v/v) and the added of serum improved the viability results after cryopReservation.

Journal ArticleDOI
TL;DR: It is investigated whether the AMP-activated protein kinase (AMPK) could play a regulatory role in the metabolic re-sculpting that occurs during freezing and the changes in phosphorylation state of translation factors are discussed in relation to the control of protein synthesis and stress-induced AMPK activation.

Journal ArticleDOI
TL;DR: The sensitivity and the permeability of Japanese flounder embryos were investigated and it was suggested that dehydration and/or permeation by cryoprotectants were insufficient for vitrification of the embryos even after they had been over-treated with the vitrification solutions.

Journal ArticleDOI
TL;DR: Experimental results show that the onset of fracturing in vitrified films of cryoprotectants is very consistent, occurring over a small temperature range, and the continuum mechanics analysis solidified the hypothesis that fracture is driven by thermal stress, not by temperature per se.