Showing papers in "Current Biology in 2001"

TL;DR: This study shows that the Drosophila insulin receptor autonomously controls cell and organ size, and that overexpression of a gene encoding an insulin-like peptide is sufficient to increase body size.

TL;DR: Central pattern generators are neuronal circuits that when activated can produce rhythmic motor patterns such as walking, breathing, flying, and swimming in the absence of sensory or descending inputs that carry specific timing information.

TL;DR: An experimental system is established, which reproduces the nuclear reprogramming of somatic cells in vitro by fusing adult thymocytes with embryonic stem (ES) cells, which shows that ES cells have the capacity to reset certain aspects of the epigenotype of somatics cells to those of ES cells.

TL;DR: It is suggested that changes in the subcellular localization of DAF-16 by environmental cues allows for rapid reallocation of resources in response to a changing environment at all stages of life.

TL;DR: It is concluded that small RNAs are much more widespread than previously imagined and that these versatile molecules may play important roles in the fine-tuning of cell responses to changing environments.

TL;DR: Homology-dependent gene silencing was shown to be used to inhibit Stellate gene expression in the D. melanogaster germline, ensuring male fertility and dsRNA-mediated silencing may provide a basis for negative autogenous control of gene expression.

TL;DR: It is shown that RILP prevents further cycling of Rab7 and induces the recruitment of functional dynein-dynactin motor complexes to Rab7-containing late endosomes and lysosomes.

TL;DR: A large-scale inactivation of the expressed genes in the nematode Caenorhabditis elegans is reported, establishing a high-throughput "RNAi-by-soaking" methodology and identifying 24 genes that might play important roles in germline development.

TL;DR: The results demonstrate that Dkk-1 is a high-affinity ligand for LRP6 and inhibits Wnt signaling by blocking Wnt-induced Fz-LRP6 complex formation, suggesting a novel mechanism for Wnt signal modulation.

TL;DR: The phenotypes of KANADI loss- and gain-of-function alleles suggest that fine regulation of these genes is at the core of polarity establishment, and they are likely to be targets of the PHB-mediated meristem-born signaling that patterns lateral organ primordia.

TL;DR: These results support a model in which cortactin modulates actin filament dendritic nucleation by two mechanisms, (1) direct activation of Arp2/3 complex and (2) stabilization of newly generated filament branch points.

TL;DR: In this article, it was shown that an early signal from the dying cell activates Rho in live neighbors to extrude the apoptotic cell out of the epithelium, and this signal is produced very early in the apoptosis process, before procaspase activation, cell shrinkage, or phosphatidylserine exposure.

TL;DR: The combined effects of transcriptional and posttranslational regulation of daf-16 transduce insulin-like signals in C. elegans and perhaps more generally is suggested.

TL;DR: In this paper, a wide variety of apoptotic cell recognition receptors have been implicated and appear to be divided into two categories, involved in tethering the apoptotic cells or triggering an uptake mechanism related to macropinocytosis.

TL;DR: It is shown that binding of APC to microtubules increases microtubule stability in vivo and in vitro and confirms the hypothesis that APC is involved in stabilizingmicrotubule ends.

TL;DR: It is shown by using mass spectrometry that histone H4, isolated from asynchronously growing human 293T cells, is methylated at arginine 3 (Arg-3) in vivo, and a novel antibody directed against hist one H4methylated at Arg-3 independently demonstrates the in vivo occurrence of this modification.

TL;DR: A critical role for BR3 in B cell ontogeny is underscored by data showing that the BR3 gene had been inactivated by a discrete, approximately 4.7 kb gene insertion event that disrupted the 3' end of theBR3 gene in A/WySnJ mice, which lack peripheral B cells.

TL;DR: In C. elegans, tol-1 is important for development and pathogen recognition, as is Toll in Drosophila, but remarkably for the latter rôle, it functions in the context of a behavioral mechanism that keeps worms away from potential danger.

TL;DR: A computational comparative genomic screen for ncRNA genes is described, to distinguish conserved RNA secondary structures from a background of other conserved sequences using probabilistic models of expected mutational patterns in pairwise sequence alignments.

TL;DR: Immunoelectron microscopy indicates that IFT52 is associated with the periphery of the transitional fibers, which extend from the distal portion of the basal body to the cell membrane and demarcate the entrance to the flagellar compartment, which suggests that the transitional fiber form a docking complex for the IFT particles destined for theFlagellum.

TL;DR: It is proposed that the abnormal time-dependent methylation events spanning the preimplantation development of clones may significantly interfere with the epigenetic reprogramming, contributing to the high incidence of physiological anomalies occurring later during pregnancy or after clone birth.

TL;DR: The ability to efficiently transfect human ES cells will provide the means to study and manipulate these cells for the purpose of basic and applied research and it is proposed that the pluripotent nature of the culture is made evident by the ability of the homogeneous cell population to form EBs.

TL;DR: It is shown here that passage through S phase in the presence of cohesin, and not cohes in per se, is essential for efficient double-strand break repair during G2 in yeast.

TL;DR: It is concluded that the interaction of Cdc42 with the partial CRIB motif of IRSp53 relieves an intramolecular, autoinhibitory interaction with the N terminus, allowing the recruitment of Mena to theIRSp53 SH3 domain.

TL;DR: Investigation of RNA-triggered events can lead to heritable changes in gene expression, and it is possible that initiation of other epigenetic phenomena such as trans-silencing and paramutation may have an RNA component.

TL;DR: It is shown for the first time that a mammalian histone variant, H2A.Z, plays a critical role in early development, and it is concluded that this hist one variant plays a pivotal role in establishing the chromatin structures required for the complex patterns of gene expression essential for normal mammalian development.

TL;DR: Characterizing the chaperone cofactors BAG-1 and CHIP gained insight into the cooperation of the molecular chaperones Hsc70 and Hsp70 with the ubiquitin/proteasome system, a major system for protein degradation in eukaryotic cells.

TL;DR: The direct effects of viewing the body on passive touch are investigated, finding that the spatial resolution of touch was better when the arm was visible than when it was not.

TL;DR: The results indicate that the complex phenotypes observed in NBS patients and cell lines may not result from a complete inactivation of NBS1 but may instead result from hypomorphic truncation mutations compatible with cell viability.

TL;DR: A role for PPARgamma is demonstrated in regulating PI 3-kinase signaling by modulating PTEN expression in inflammatory and tumor-derived cells.