Showing papers in "Developmental Biology in 1985"

TL;DR: Five genes are identified that affect the growth of the amphid and phasmid axons and mechanosensory PDE neurons as well, and the unc-33 mutation specifically affects neuronal microtubules.

TL;DR: The chicken egg acquires about 99% of its size during the 6 days before laying (Romanoff and Romanoff, 1949), when approximately 1 g protein is accumulated per day (Cutting and Roth, 1973).

TL;DR: Development of the insect CNS depends on cell interactions and positional cues to create a pattern of NBs, and then on cell lineage to restrict the fate of the NB progeny.

TL;DR: It is proposed that primary sensory neurons, regardless of their embryological origin, are responsive to a "central-target" (CNS) derived neurotrophic factor--BDNF, while they are differentially responsive to "peripheral-target"-derived growth factors, such as NGF, depending on whether the neurons are of neural crest or placodal origin.

TL;DR: Grafting of the neural plate area adjacent to the "ridge" territory containing the placodal ectoderm revealed that the presumptive region of the hypothalamus is in contiguity with that of the adenohypophyseal placode.

TL;DR: Three aspects of neurogenesis are examined, including the morphological changes associated with the differentiation of neuronal precursor cells and nonneuronal support cells and the characteristic temporal sequence of differentiation in the stereotyped pattern of NBs.

TL;DR: In this article, the authors used three antibodies (anti-galactocerebroside (GC), anti-glial fibrillary acidic protein (GFAP), and the A2B5 monoclonal antibody) to examine the timing and sequence of the development of the three types of glial cells in vivo.

TL;DR: All-trans-retinoic acid closely mimics the putative morphogen that has been postulated to be emitted by polarizing region cells during normal development.

TL;DR: Observations indicate that mechanisms involving the movement of the meiotic spindle to the oocyte cortex and development and maintenance of cortical polarity are cytochalasin B sensitive.

TL;DR: Cell lineages during development of ascidian embryos were analyzed by injecting horseradish peroxidase as a tracer enzyme into identified cells of the 16-cell and 32-cell stage embryos of Halocynthia roretzi, indicating that the stage at which the fates of every blastomere are determined to one tissue is later than the 64- cell stage.

TL;DR: Immunoblot analyses of tissue extracts and cell culture media indicated the 150-kDa form of nidogen as the largest and predominant form in all tissues examined andEquimolar amounts of laminin and ndogen were determined in 6 M guanidine X HCl extracts of tissues by radioimmunoassays, further indicating stoichiometric complexes.

TL;DR: Results show that growth cones are not guided by contact with oriented extracellular fibrils, aligned mesenchyme cells, the myotome, or the vasculature, and it is proposed that the anatomical pattern of outgrowth is determined by the distribution of preferred substrata, the most preferred substratum being other neurites.

TL;DR: In this paper, the maturation-inducing activity of residues at various steps of purification was assessed by an in vitro germinal vesicle breakdown (GVBD) assay based on fully grown prophase-arrested folliculated oocytes of amago salmon.

TL;DR: Fluorescent dextran-amine tracers allow embryonic cell lineages to be studied in fixed, permeabilized, or sectioned embryos and are brighter than commercially obtainable products.

TL;DR: A large fraction of both proteases was released by distal processes and/or growth cones suggesting that these proteases could be involved in growth cone functions.

TL;DR: Cloned DNA sequences were introduced into the cytoplasm of unfertilized sea urchin eggs by a simple microinjection technique, and sperm was then added, and development allowed to proceed.

TL;DR: Patterns of cleavage and cytoplasmic connections between blastomeres in the embryo of the zebrafish, Brachydanio rerio have been described, and alternative patterns of the 5th cleavage are described.

TL;DR: The temporal and spatial distribution of short chain skeletal collagen was immunohistochemically examined in the chick tibiotarsus from 6 days of embryonic development to 1 day posthatching, consistent with previous biochemical studies suggesting that, in cartilage, type X collagen is specifically a product of that population of chondrocytes which have undergone hypertrophy.

TL;DR: Neither "compartment" nor "strict lineage" models are sufficient to explain the origin of the YSL, and it is proposed that the position of a cell on the blastoderm margin, and not the cell's lineage, determines YSL cell fate.

TL;DR: The results obtained with cGK suggest a transitory intrinsic heterogeneity in the immature cerebellar cortex and a transient biochemical compartmentalization resulting from the differential expression of parts of the same genotype by clusters of PCs.

TL;DR: The results indicate that the previously defined loss of bulk RNA and changes in the state of adenylation of mRNA during the first 11/2 days of embryogenesis actually begin during the 12 hr of meiotic maturation preceding fertilization.

TL;DR: The expression of a cell adhesion molecule called uvomorulin during morphogenesis of the embryonic kidney has been studied in this article, showing that it plays a role in the initial adhesion of the differentiating tubule cells.

TL;DR: In vitro transcription experiments demonstrate that laminin and collagen IV are transcriptionally regulated by RA; but2cAMP also enhances the rate of transcription of these genes in F9 cells.

TL;DR: The larval hyp7 syncytium comprises a fixed number of diploid embryonic nuclei plus an increasing number of tetraploid postembryonic nuclei and may correspond to nuclear or cellular divisions in another nematode Panagrellus redivivus.

TL;DR: SDS-polacrylamide gel electrophoretic patterns of various size follicles indicated that five major protein bands accumulate and presumably are proteolytically derived from a 200-kDa vitellogenin precursor and presumably help generate part of the osmotic pressure gradient required for water uptake during oocyte maturation.

TL;DR: The authors examined changes in the hyaluronate-dependent pericellular matrices elaborated by mesodermal cells of the limb bud from different developmental stages and the corresponding changes in production of cell surface-associated and secreted glycosaminoglycans.

TL;DR: A difference is shown in regulation of the synthesis and accumulation of testis-specific histones H1t, TH2A,TH2B, and TH3, which does not accumulate until the pachytene stage of spermatogenesis.

TL;DR: The data indicate that as the presumptive mesenchyme cells leave the vegetal plate of the embryo they lose affinities for hyalin and for neighboring cells, and gain an affinity for fibronectin associated with the basal lamina and extracellular matrix that lines the blastocoel.

TL;DR: It is concluded that overgrowth in this mutant is due to an autonomous defect in the imaginal primordia, which requires an extended larval period for its expression in situ.

TL;DR: It is concluded that genotypes of Convolvulus arvensis are blocked in the acquisition of competence, because explants of some of these genotypes can be made to regenerate organ types by short precultures on seemingly inappropriate media.