Showing papers in "Developmental Biology in 2002"

TL;DR: It is found that the zebrafish fli1 promoter is able to drive expression of enhanced green fluorescent protein (EGFP) in all blood vessels throughout embryogenesis, and these transgenic lines allow detailed analysis of both wild type and mutant embryonic vasculature.

TL;DR: A transgenic mouse line is generated in which Cre-ER is ubiquitously expressed to permit temporally regulated Cre-mediated recombination in diverse tissues of the mouse at embryonic and adult stages and this inducible Cre system will be a broadly useful tool to modulate gene activity in mouse embryos, adults, and culture systems where temporal control is an important consideration.

TL;DR: This is the first complete and detailed analysis of the epigenetic reprogramming cycle during preimplantation development and shows that demethylation of the male pronucleus is completed within 4 h of fertilisation.

TL;DR: Forkhead proteins are not among the largest transcription factor families, but display a remarkable functional diversity and are involved in a wide variety of biological processes.

TL;DR: Detailed analysis of neural crest migration pathways using X-gal staining, and mesodermal tracing by DiI labelling, shows that the neural crest-mesodermal tissue juxtaposition that later forms the coronal suture is established at E9.5 as the caudal boundary of the frontonasal mesenchyme.

TL;DR: The insights in the extracellular regulation of members of the BMP subfamily of secreted growth factors with a major emphasis on vertebrate BMP modulation are reviewed.

TL;DR: The evidence so far suggests that, with careful controls, morpholinos provide a relatively simple and rapid method to study gene function.

TL;DR: The anatomy of the mouse placenta was examined by preparing plastic vascular casts and serial histological sections of implantation sites from embryonic day (E) 10.5 to term to provide important insights into how the fetoplacental unit interacts with the maternal intrauterine vascular system during pregnancy in mice.

TL;DR: The Sca-1(pos) population is enriched for functional stem/progenitor cells, as demonstrated by its increased regenerative potential when transplanted into the cleared mammary fat pads of host mice.

TL;DR: It is shown that lin-28 is repressed during normal development by a mechanism that acts on its mRNA after translation initiation, and that a parallel lin-4-independent regulatory mechanism regulates the expression of lin-14, which encodes a nuclear hormone receptor.

TL;DR: There is strong evidence that a single Ca(2+) transient-driven signaling system can initiate and drive a cell into a new developmental pathway, as well as can account for the temporal sequence of cellular changes associated with early development.

TL;DR: It is concluded that AKAP4 is a scaffold protein required for the organization and integrity of the fibrous sheath and that effective sperm motility is lost in the absence ofAKAP4 because signal transduction and glycolytic enzymes fail to become associated with the fibrus sheath.

TL;DR: Current evidence from studies of ascidian and mammalian fertilization favors a fusion-mediated mechanism; this is supported by experiments indicating that injection of sperm extracts into eggs causes Ca(2+) release by the same pathway as fertilization.

TL;DR: The current form of a provisional DNA sequence-based regulatory gene network that explains in outline how endomesodermal specification in the sea urchin embryo is controlled is presented.

TL;DR: This work compares and contrast the mechanisms involved in heart formation in fruit flies and mammals in the context of a network of transcriptional interactions and point to unresolved questions for the future.

TL;DR: It is concluded that the TOP-dGFP reporter line faithfully illustrates domains of beta-catenin activity and enables the identification of responsive cell populations.

TL;DR: Drosophila appears as a good in vivo model to study morphogenetic processes requiring epithelial regeneration such as wound healing in vertebrates and the embryonic JUN N-terminal kinase gene cassette is induced at the edge of the wound.

TL;DR: It is shown that Wnt5a is expressed in the mouse lung and plays an important role in lung distal morphogenesis and the overall architecture of the mutant lungs is characterized by overexpansion of the distal airways and inhibition of lung maturation as reflected by persistence of thickened intersaccular interstitium.

TL;DR: It is reported that parasitization by the wasp Leptopilina boulardi leads to a dramatic increase in the number of both lamellocytes and crystal cells in the Drosophila larval lymph gland, and proposed that, by affecting a specific pool of hematopoietic precursors, this pathway thus confers immune capacity to third-instar larvae.

TL;DR: It is found that oligodendrocytes develop similarly in zebrafish embryos, in that they arise from ventral spinal cord and migrate to new positions and one possibility consistent with these data is that Hedgehog signaling, partly by inducing olig2 expression, specifies neural precursor cells that have potential for primary motor neuron or oligodendedrocyte fate.

TL;DR: The following review focuses on an emerging field, the goal of which is to understand the influence of bacteria on animal developmental programs, and the current status of the field is reviewed and the emerging research horizons are examined.

TL;DR: Both TGF beta2 and TGFbeta3 appear necessary for the full morphogenetic program of EMT in the chick, but only TGFBeta2 is expressed and obligatory for mammalian endocardial cushion cell transformation.

TL;DR: The regulatory network subelement emerging from this work shows how the specificity of micromere function depends on continuing global regulatory interactions, as well as on early localized inputs.

TL;DR: It is demonstrated that Shh from the urethral epithelium is required for outgrowth, patterning, and cell survival in the developing external genitalia in mice with a targeted deletion of Shh.

TL;DR: A unifying model where lateralizing and posteriorizing signals are presented as two stages of the same inductive process required for neural crest induction is proposed.

TL;DR: The cloned Xenopus Strabismus (Xstbm), a homologue of the Drosophila planar cell or tissue polarity gene, is cloned and the role of Xstbm in regulating the cell polarity driving convergence and extension in Xenopus is characterized.

TL;DR: Results suggest that, provided sufficient ATP is present and pH is sufficiently alkaline, Ca2+ switches on hyperactivation by enabling curvature of the principal bends to increase.

TL;DR: Both genes were coexpressed in cartilaginous condensations, indicating a cooperative role in the regulation of early chondrocyte differentiation and thus explaining the expansion/maintenance of cartilage in the carpus and joints of infected chick limbs.

TL;DR: It is suggested that the proper development of ventricular myocardium is dependent on the invasion of undifferentiated, WT1-positive, retinoic acid-synthesizing EPDCs, and it is proposed that an interaction between E PDCs and endocardial (derived) cells is imperative for correct development of the AV cushions.

TL;DR: The fetal epicardium secretes trophic factors which drive fetal cardiomyocyte proliferation and promote ventricular chamber morphogenesis, in response to retinoic acid and in a manner requiring the activity of RXRalpha.