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Showing papers in "Enzyme and Microbial Technology in 1979"


Journal ArticleDOI
TL;DR: Thermophilic bacteria have application in chemical feedstock and fuel production, bioconversion of wastes, enzyme technology, and single cell protein production and facilitate reactant activity and product recovery.

226 citations


Journal ArticleDOI
TL;DR: Non-aqueous solvents are being extensively used in cryoenzymology as antifreeze agents, in solubilizing and purifying enzymes, and to a lesser degree in two-phase systems in which the non-polar substrate is dissolved in the non -aqueous phase.

174 citations


Journal ArticleDOI
TL;DR: Hydrogenase is an enzyme which reversibly activates molecular hydrogen and has potential applications in the production of hydrogen by solar energy, and methods employed for assay of the enzyme are described.

87 citations


Journal ArticleDOI
TL;DR: In this article, a review deals with the problem of enzyme stabilization without the use of carriers, and the following main types of stabilization are considered: addition of low-molecular weight compounds; addition of organic solvents; chemical modification of key functional groups on enzymes; intramolecular crosslinking with bifunctional reagents.

73 citations


Journal ArticleDOI
TL;DR: E. coli cells immobilized with к-carrageenan and treated with glutaraldehyde and hexamethylenediamine showed the highest productivity, and was compared for production of l - aspartic acid.

72 citations


Journal ArticleDOI
TL;DR: The potential application of this enzyme for the preparation of some d -amino acids used as pharmaceutical intermediates is discussed.

65 citations


Journal ArticleDOI
TL;DR: Heterocystous nitrogen-fixing blue-green algae consist of filaments containing two types of cells: the heterocysts, responsible for ammonia synthesis, and vegetative cells, which exhibit normal photosynthesis and reproductive growth.

53 citations


Journal ArticleDOI
TL;DR: Plant proteases, characterized by the reduced sulphydryl group required for catalysis, can be produced from tropical plants and are used in a variety of food industries as discussed by the authors.

53 citations


Journal ArticleDOI
TL;DR: It is suggested that this increased sensitivity eliminates the logarithmic response predicted by the Nernst equation for a pH-based enzyme electrode.

49 citations



Journal ArticleDOI
TL;DR: In this article, a constant strain rate and stress relaxation tests on columns of spherical alginate pellets containing entrapped microbial cells were performed, showing that the columns were relatively resistant to compression over long periods.

Journal ArticleDOI
TL;DR: A review of the role of enzymatic epoxidation reactions in the metabolism of polycyclic aromatic hydrocarbons, in steroid biosynthesis and interconversion, and in various other pathways is presented.

Journal ArticleDOI
TL;DR: It is demonstrated that the dehydrogenase assay can provide a valid indication of soil microorganism activity, and the use of the assay could be advocated in determining soil microbial activity in ecological investigations.

Journal ArticleDOI
TL;DR: The reactor was successfully operated for more than 5 weeks for the isomerization of demineralized concentrated whey hydrolysate of 8% lactose, 23% galactose, and up to 36% added glucose to obtain a syrup of sweetness approaching that of sucrose.

Journal ArticleDOI
TL;DR: The predictive capacity of the collocation method in estimating steady-state and operational effectiveness factors for enzyme reactors is demonstrated and these factors can be used to predict the performance of continuous stirred tank and plug-flow reactors.

Journal ArticleDOI
TL;DR: In this paper, homogeneous and heterogeneous biocatalysis tools for barley starch syrup production were investigated, and it was concluded that barley starch may be used as an alternative raw material for biOCatalytic starch syrup synthesis.

Journal ArticleDOI
TL;DR: In this paper, a reactive polymer polysuccinimide was aminolysed with the spin label 2,2,6,6-tetramethyl 4-aminopiperidine-N -toxide to obtain on average about 9 unpaired spins/macromolecule.


Journal ArticleDOI
TL;DR: Recalcitrant compounds can be made biodegradable by developing microorganisms capable of degrading the compound and by treating the compound to make it more conducive to mirobial attack.

Journal ArticleDOI
TL;DR: The use of date juice as a substrate for single cell protein production was investigated and Saccharomyces cerevisiae ATCC 4111 gave the highest protein and cell production and the optimum substrate concentration was 4 - 5% soluble solids.

Journal ArticleDOI
TL;DR: Four commercial instruments that measure glucose by incorporation of immobilized reagents are described and compared and the design of the immobilized enzyme or enzymes is shown to be related to the type of instrument.

Journal ArticleDOI
TL;DR: The usefulness of ultrafiltration for obtaining bland protein hydrolysates seems to be limited to short-term processes with cellulose acetate membranes.


Journal ArticleDOI
TL;DR: In this article, Emery et al. found that materials that have been treated with titanium (IV) chloride by the method of Emery and Waters this article possess a surface layer of hydrous titanium oxide which is able to bind protein in a manner analogous to that of free hyrous titanium(IV) oxide, which may be removed by exposure to acidic hydrogen peroxide solution.

Journal ArticleDOI
TL;DR: On-line measurement of enzyme activity was successfully performed and the measured activity values were corrected against a standard sample of known activity by means of a mathematical model for the calibration curve which was stored in the computer.

Journal ArticleDOI
TL;DR: Microbial cells possessing high urease activity were entrapped in cellulose triacetate fibres, and their capability to hydrolyse urea was evaluated, finding the best cells-to-carrier ratio was 1:10 and the optimum pH and temperature were 7.2 and 60°C respectively, which suggests that the cells are physically bound to the polymer matrix.

Journal ArticleDOI
TL;DR: In this paper, the potential for carbohydrate fermentation as an alternative process technology for bulk organic chemicals is examined and the significance of their potential impact on the evolving organic chemical industry is projected, including the new development directions for chemicals derived directly and indirectly from carbohydrate fermentation.

Journal ArticleDOI
TL;DR: In this paper, the authors determined the optimum conditions for the immobilization of glucoamylase on glass involving diazotized 5-aminosalicylic acid bonded to a deposited imperfectly crystallized film of TiO 2.

Journal ArticleDOI
TL;DR: Microsomal enzymes have some unique properties which lend themselves to applications in the areas of pharmacology, medicine, and identification of potentially carcinogenic or toxic metabolites of foreign compounds.

Journal ArticleDOI
TL;DR: In this paper, the effect of increasing ethanol concentration in the feed on the growth and physiological activity of the yeast Candida utilis was studied and it was shown that the presence of acetate in the medium inhibits the respiration activity of yeast growing on ethanol.