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Showing papers in "Enzyme and Microbial Technology in 1998"


Journal ArticleDOI
TL;DR: By cell entrapment within PVA cryogel matrices, diverse immobilized biocatalysts were prepared which were capable of performing both a simple monoenzyme biotransformation of respective substrates and a complex biosynthesis of various high- and low-molecular-weight products, decompose pollutants, and operate in biosensors.

388 citations


Journal ArticleDOI
TL;DR: Toxicity of triphenylmethane dyes is discussed with respect to different organisms and some aspects of biodegradative products of this group of dyes are also mentioned.

331 citations


Journal ArticleDOI
TL;DR: Correlations between the cellular toxicity or the extractive capacities of different solvents and some of their physical properties have been proposed in order to minimize preliminary, solvent-selection experimental work but also to help in the understanding of the molecular mechanisms of toxicity and extraction.

299 citations


Journal ArticleDOI
TL;DR: The laccase of Trametes versicolor was able to oxidize in vitro most of the 14 polycyclic aromatic hydrocarbons (PAH) tested as mentioned in this paper.

299 citations


Journal ArticleDOI
TL;DR: A review of reaction systems employed in ester production catalyzed by lipases can be found in this paper, where water has a profound effect on the lipase behavior either directly by affecting the hydration of the enzyme or indirectly by changing the nature of the reaction media and enzyme support materials.

273 citations


Journal ArticleDOI
TL;DR: Bacillus species producing a thermostable phytase was isolated from the soil of a Korean cattle shed and was very specific for phytate and had little or no activity on other phosphate esters.

223 citations


Journal ArticleDOI
TL;DR: The properties of various phytases are summarized and the digestion of phytate by phytase and its products are also discussed.

198 citations


Journal ArticleDOI
TL;DR: The present review attempts to summarize the reported data concerning the bacterial producers of CGTase, growth cultural conditions providing optimal enzyme biosynthesis in batches, repeated batch and continuous cultivation of free and immobilized cells, as well as some physicochemical and biochemical characteristics of the enzyme, C GTase immobilization, and enzyme structure.

181 citations


Journal ArticleDOI
TL;DR: By using DAAO/catalase-coimmobilized derivatives, more than 98% of phenylpyruvic acid was obtained after complete oxidation of d-phenylalanine by using the racemic mixture as substrate, which prevented enzyme from inactivation.

149 citations


Journal ArticleDOI
TL;DR: In this article, the enantioselective esterification of ibuprofen (2-(4-iso-butylphenyl)propionic acid), catalyzed by Candida antarctica lipase (type B), was performed in various organic solvents.

139 citations


Journal ArticleDOI
TL;DR: A Bacillus amyloliquefaciens strain isolated from soil produced xylanolytic enzymes in the extracellular medium when grown on xylan and nitrate and Tryptophan seems to be essential for xylanase activity.

Journal ArticleDOI
TL;DR: Water-soluble peptides from Mozzarella, Italico, Crescenza, and Gorgonzola cheeses were fractionated by reverse-phase fast protein liquid chromatography as discussed by the authors.

Journal ArticleDOI
TL;DR: Horseradish peroxidase has been proven effective in removing phenolic compounds in wastewater and additives such as polyethylene glycol have been found very effective in reducing the minimum enzyme dose required.

Journal ArticleDOI
TL;DR: The kinetic model of lactose hydrolysis by means of a commercial β-galactosidase from Kluyveromices fragilis provided by Novo nordisk has been determined using a wide range of the main variables: enzyme, substrate, and product concentrations and temperature.

Journal ArticleDOI
TL;DR: Production of a lipopeptide surfactant, surfactin, by Bacillus subtilis ATCC 21332 was highly enhanced when iron concentration in the medium was raised from 4 μ m to the few m m level, which resulted in increased biomass concentration.

Journal ArticleDOI
TL;DR: All the polymers synthesized were found to be quite homogeneous and their average molecular weight is of the same order of magnitude as the ones commercially available.

Journal ArticleDOI
TL;DR: In this article, the adsorption of a purified 20.7 kDa thermostable endo-1-4-β-xylanase (EC 3.1.8) from a Bacillus sp. on wheat straw at 4°C was studied.

Journal ArticleDOI
TL;DR: Mixed fungal cultures have been isolated from acidic gasworks soil by their ability to utilize iron or nickel cyanide as the sole source of nitrogen at acidic or neutral pH, respectively, and growth was associated with progressive removal of cyanide from the culture supernatant.

Journal ArticleDOI
TL;DR: The nitrogen demand of industrial yeast strains were compared and substantial differences were found between strains, regardless of the initial medium composition and added nitrogen source.

Journal ArticleDOI
TL;DR: The oxygen transfer coefficient increases with the agitation rate and the oxygen consumption rate increased almost linearly with the biomass concentration; however, substrate consumption was highest at HOT conditions.

Journal ArticleDOI
TL;DR: The existence of at least two different alpha-galactosidases in Aspergillus niger was confirmed, with one of them showing synergism with beta-mannanase and the other with a different mass after deglycosylation with N-glycanase F.

Journal ArticleDOI
TL;DR: Fedbatch culture proved to be a better fermentation system for the production of lactic acid than batch culture.

Journal ArticleDOI
TL;DR: Ice-nucleation protein (INP), an outer membrane protein from Pseudomonas syringae, is able to catalyze the ice crystal formation of supercooled water and could also direct CMCase on the cell surface, suggesting that it has the secretion and targeting signal to the outer membrane.

Journal ArticleDOI
TL;DR: The deproteinization activity was better than that of the proteolytic bacterium Pseudomonas maltophilia reported in many previous studies and ranged in the order of higher protein removal efficiency.

Journal ArticleDOI
TL;DR: The decarboxylase was inactive toward both o - and m -coumaric acid and toward cinnamic acid, indicating that the para -hydroxy group is essential for activity.

Journal ArticleDOI
TL;DR: In this paper, the delignification of chemical pulps with laccase and n -hydroxybenzotriazole was explored employing a pre-and post-O 2 delignified softwood draft pulp.

Journal ArticleDOI
TL;DR: In this paper, the performance of Bacillus subtilis strain 11089 was studied in terms of their chemical compositions and their ability to support the growth and extracellular hydrolytic enzyme production.

Journal ArticleDOI
TL;DR: Comparative and standardized treatments of pulp with the commercial bleach-boosting enzyme preparations Cartazyme and Pulpzyme demonstrated that the crude S. thermoviolaceus xylanase preparation was at least as effective in enhancing brightness with concomitant preservation of paper strength properties.

Journal ArticleDOI
TL;DR: A Bacillus licheniformis mutant derived by random mutagenesis with n -methyl- n ′-nitro- n -nitrosoguanidine treatment producing high levels of the lipopeptide biosurfactants was selected by an ion-pair plate assay.

Journal ArticleDOI
TL;DR: Glucoamylase was covalently immobilized onto pHEMA/EGDMA microspheres of two different sizes: 50–100 μm and 100–200 μm in diameter, indicating decreased affinity by the enzume for its substrate.