Showing papers in "Enzyme and Microbial Technology in 1999"

TL;DR: In this paper, the influence of dilute sulfuric acid hydrolysis of spruce (softwood) on sugar yield and on the fermentability of the hydrolysate by Saccharomyces cerevisiae (Baker's yeast) was investigated.

TL;DR: The choice of buffer had a profound effect on pH stability upon dye addition, and consequently, decoloration, and the use of 2,2′-dimethylsuccinic acid allowed for excellent pH control and resulted in highDecoloration ability.

TL;DR: The dielectric properties of cells and their components and the electrical techniques that use them will be discussed mainly in the context of biotechnology but some applications in medicine will also be highlighted.

TL;DR: In this article, a number of soil and water samples were collected from the vicinity of effluent treatment plant of a textile and dyeing industry and several organisms were screened for their ability to decolorize triphenylmethane group of dyes.

TL;DR: In this article, the degradation of enzymatic activity was attributed to the formation of an hydrophilic hindrance around the enzyme resulting in diffusion limitations of the hydrophobic substrate from the organic phase to the enzyme.

TL;DR: In this article, a central, composite experimental design was used to optimize the composition of the culture medium for maximizing the productivity of PHB in batch culture of Alcaligenes latus, American Type Culture Collection 29713.

TL;DR: This review introduces organic media bioc atalysis before addressing the state of the art of recent fundamental and applied aspects of extremozyme biocatalysis, and enhancing extremo enzyme performance via chemical modification, complexation, immobilization, and protein engineering.

TL;DR: The influence of sodium hydroxide addition during the thermo-chemical pretreatment of a microbial biomass has been evaluated in terms of particulate chemical oxygen demand (COD) solubilization and anaerobic biodegradability as discussed by the authors.

TL;DR: In this paper, the thermal stability of C. antarctica lipase B (CALB) was studied and compared to adsorbed derivatives from Novo Nordisk at 50°C under wet conditions.

TL;DR: In this paper, the authors evaluated five different methods of enzyme immobilization on silicon substrates, including metal link coupling, entrapment, gelatin, poly-l -lysine adsorption, and pendant amino or epoxide linkers.

TL;DR: It is concluded that the inhibition of metabolic intermediates plays a crucial role in phenol degradation modeling, especially over a wide concentration range of phenol.

TL;DR: A link between the enhanced production of Surfactin and the onset of nitrate-limited growth is demonstrated, by using a defined medium with ammonium nitrate as a nitrogen source.

TL;DR: The use of solid-state fermentation for the production of xylanase by Bacillus sp.

TL;DR: It was concluded that the external supply of CO2 and H2 at the optimum ratio makes it possible to produce succinic acid with a high yield and productivity.

TL;DR: An extracellular lipase of Streptomyces rimosus R6-554W was isolated from the culture filtrate by column chromatography and was shown to be a monomeric, basic protein active toward triolein and p-nitrophenyl esters, with preference for those with medium size acyl chain length.

TL;DR: Three different reactor configurations were designed and tested for decolorization of an azo dye, Orange II, with white rot fungus and all the bioreactors showed high and stableDecolorization activity in long-term operation.

TL;DR: The thermophile character of this strain (growth at 52°C) proves that it is particularly adapted for industrial production of lactate without sterile conditions, and the factorial fractional experimental design was performed to optimize batch fermentation.

TL;DR: A wild-type Coprinus cinereus laccase and its recombinant form expressed in an Aspergillus oryzae host have been purified and characterized, and was able to oxidatively bleach Direct Blue 1 dye at alkaline pH, making it a promising industrial enzyme candidate.

TL;DR: Changes in the initial pH, lowering or raising it, lowered the enzymes production, but favored fungus growth, whereas the mycelial protein value was higher.

TL;DR: In this article, a continuous degradation of phenol from 100 mg L -1 to concentrations as low as 2.5 mg L −1 using immobilized Pseudomonas putida was established.

TL;DR: Corrections for solvation are made with the new model for the esterification in toluene, hexane, trichloroethane, and diisopropyl ether, which results in kinetic constants that deviate less from the average value.

TL;DR: A group of 17 strains of the thermophilic fungus Thermomyces lanuginosus was examined for the production of xylanases, β-mannanases, arabinanase, and pectinases, and several were proven to be outstanding producers of microbial xylanase on glucuronoxylan and corn cobs.

TL;DR: In this paper, the enzyme was identified as a glycoprotein with pI of 4.6 and the N-terminal amino acid sequence data (19 residues) and the amino acid composition were determined.

TL;DR: In this paper, the effect of gross fiber characteristics on enzyme accessibility and hydrolysis of Douglas fir kraft pulp substrates was investigated and it was found that the average fiber size and coarseness of the substrate had a significant effect on the enzyme adsorption capacity.

TL;DR: Mycelial pellets of Trametes versicolor decolorized successive additions of the same dye (Amaranth) and of different dyes and dye mixtures, indicating that dye decoloration by T.versicolor may be distinct from lignin and pollutant degradation by P. chrysosporium.

TL;DR: expression levels of cellulolytic enzymes, cell growth, and ethanol production in cellulose-containing media were significantly increased in comparison with plasmid-based expression and maintenance of the integrated genes was perfect up to 50 generations even in nonselectable media.

TL;DR: A fairly complete embedding of the whole enzyme surface in a highly hydrophilic nano-environment seems to be achieved.

TL;DR: The addition of 6% (w/w) methanol into the substrate increased the concentration of citric acid from 176 to 264 g kg−1 dry pod, and the maximum citricacid concentration was obtained at a particle size of 0.5 mm.

TL;DR: A mathematical model is developed to estimate the irradiance profile and average irradiance inside the culture, and hence, to compare the biomass production capability of the airlift device with a horizontal-loop tubular photobioreactor (HLTP) located at the same place as the ALP.

TL;DR: Almost complete removal of the colored quinones and associated polymers from the permeate was observed and this membrane allows greater flux and was shown to facilitate high efficiency in removal of reaction products from the reactor.