Showing papers in "Enzyme and Microbial Technology in 2003"

TL;DR: The ability of white-rot fungi to adsorb and accumulate metals together with the excellent mechanical properties of fungal mycelial pellets provide an opportunity for application of funGal mycelia in selective sorption of individual heavy metal ions from polluted water.

TL;DR: A review of the most commonly used biosensor systems based on their transducer properties, which include surface plasmon resonance (SPR), amperometric, potentiometric, and acoustic wave sensors and their applications for the detection of pathogens in food and water is presented in this paper.

TL;DR: An overview of recent developments on the use of microorganisms for steroid production is presented in order to increase the efficiency of the existing processes as well as to identify new potentially useful bioconversions.

TL;DR: In this article, the effect of organic solvent on alcoholysis rate was investigated and highest conversion (80%) was found in n -hexane and petroleum ether, and the best conversion (>90%) was with lipase from Pseudomonas fluorescens (Amano AK) at the highest molar equivalent of oil:methanol (1:4.5).

TL;DR: This article surveys the enzyme stabilization literature since 1997, focusing on protein engineering and chemical modification, and case studies focus on enzymes used, or potentially useful, in industrial processes or in biosensors.

TL;DR: The enzyme activity was increased up to more than two-fold in presence of 2-mercaptoethanol, glutathione, and dithiothreitol, suggesting it to be a thiol-dependent serine protease.

TL;DR: In this paper, the authors studied the effect of temperature and enzyme concentrations on porous media permeability, using enzymatic formation of CaCO 3 in a core-flooding system with Berea sandstone cores.

TL;DR: Two alkaline protease producing alkaliphilic bacterial strains, designated as AL-20 and AL-89, were isolated from a naturally occurring alkaline habitat and the protease it produce offers an interesting potential for the enzymatic and/or microbiological hydrolysis of feather to be used as animal feed supplement.

TL;DR: Production of lovastatin and microbial biomass by Aspergillus terreus ATCC 20542 were influenced by the type of the carbon source and the nitrogen source used, and the use of spores gave a more consistent inoculum in the different runs.

TL;DR: The current state-of-the-art means for the production of plasmid DNA at large scale in compliance with existing regulatory guidelines is addressed, along with the effect of varying cultivation conditions on final plasmids content.

TL;DR: An organic solvent-tolerant bacterium producingorganic solvent-stable protease was isolated from fishing industry wastewater and identified as B. cereus, suggesting that the preparation contains metalloprotease(s).

TL;DR: The effect of the non-ionic surfactant Tween-20 as an additive in SSF had several positive effects on SSF: the ethanol yield was increased by 8%; the amount of enzyme loading could be reduced by 50%, while maintaining a constant yield.

TL;DR: Bovine liver catalase was immobilized into chitosan beads prepared in crosslinking solution Various characteristics of immobilized catalases such as the pH-activity curve, the temperature-active curve, thermal stability, operational stability, and storage stability were evaluated.

TL;DR: In this article, the influence of different wastewater compositions on the extracellular polymer substances (EPS) and physicochemical properties (surface charge, bound water and contact angle) were studied in continuous fed completely stirred tank reactor (CSTR).

TL;DR: In this article, a transesterification of n-octanol with vinyl acetate was studied at 30°C as a model reaction by employing different lipases as catalysts such as Psedomonas species lipase immobilized on diatomite, free Candida rugosa lipase.

TL;DR: The effect of inhibitors on the recombinant xylose fermenting strain Saccharomyces cerevisiae 259ST was compared to three reference strains and was found to be as hardy as the reference strains towards acetic acid, ammonium, furfural, and osmotic effects, which are inhibitory compounds in SSL.

TL;DR: The degree of resistance of different yeast strains was highly dependent on the inhibitor used, and a mutant strain of S. cerevisiae displaying enhanced resistance against coniferyl aldehyde compared with the parental strains was identified.

TL;DR: Yeast strain Y68, producing a large amount of pullulan, was isolated from the leaves collected in the south of China and no pigment in the medium was observed during the fermentation, suggesting that strain Y 68 was a non-pigmented yeast strain.

TL;DR: The growth and enzyme production by Trichoderma reesei Rut C-30 using different lignocellulosic materials as carbon source were investigated and it was found that endoglucanase and endoxylanse activities were produced throughout the cultivations, whereas α-arabinosidase was induced late during the cultivation.

TL;DR: Peptide mapping of the 18 and 16 kDa polypeptides from POxA3a and POXA3b suggests the identity of the18 kDa subunits and the generation of the 16 k da polypePTides from the 18 kDa ones, while structural data does not allow ascertaining significant differences between the two isoenzymes.

TL;DR: In this article, a wild strain of Penicillium sp in solid-state fermentation (SSF) was used as carbon and nitrogen source and solid matrix for synthesizing a serine protease.

TL;DR: The cell growth of G. lucidum was significantly inhibited when DOT was controlled ∼10% of air saturation, which was due to the oxygen limitation in mycelia aggregates, and the total production and productivity of IPS and GA at a low DOT were lower than those at a high DOT.

TL;DR: Five groups of polysaccharides were prepared from mycelium extract and top and bottom fraction of filtrate precipitates by submerged culture of Grifola frondosa at two different media and their individual biological activities were studied.

TL;DR: The application of this NADH oxidase for the regeneration of NAD is demonstrated by the simultaneous coupling with an NAD-dependent R -specific alcohol dehydrogenase and the R -enantiomer could be oxidized completely resulting in a pure S -alcohol.

TL;DR: The filamentous fungus Penicillium brasilianum IBT 20888 was cultivated on a mixture of 30 g l−1 cellulose and 10 g xylan for 111 h and the resulting culture filtrate was used for protein purification and revealed similarity in molecular mass, pI and hydrolytic properties suggested that these two enzymes were identical, but the smaller was lacking the cellulose-binding domain or an essential part of it.

TL;DR: In this article, the laccase-catalyzed polymerization of aniline was performed in the presence of sulfonated polystyrene (SPS) as a template.

TL;DR: In this paper, an emulsion consisting of transesterified rapeseed oil, a surfactant and a slurry of C. vulgaris was used as a fuel in an unmodified single cylinder diesel engine.

TL;DR: Dye decolourisation by CNPR 8107 was more favourable at 30 than at 37 °C, and compared favourably with P. chrysosporium as well as a reference commercial strain T. versicolor ATCC 20869, however, CNPR8107 exhibited an initial lag in its dye decolouredisation rate due to lower laccase production than ATCC20869.

TL;DR: In this paper, a copolymerization of vinyl acetate and divinyl benzene using oleic acid-stabilized magnetic colloids as magnetic cores was described by the Langevin function.

TL;DR: A broad screening of 58 fungi including 46 white rot strains on solid media resulted in the selection of 13 white rot fungi, which were active in decolourising the poly R-478 lignin model and were capable of growing on crude olive oil mill wastewaters (OOMW) with a COD of 100 ǫl −1.