Showing papers in "Enzyme and Microbial Technology in 2005"

TL;DR: The solvent properties of ionic liquids, their effects on enzyme performance such as activity, stability and selectivity, and their applications in biocatalysis are discussed.

TL;DR: Three different generations of microbial fuel cell, distinguished by their historical development and mechanisms of electron transfer, were compared and it is shown that Gen-II and -III but not Gen-I may be used advantageously in wastewater treatment and power generation from organic matter.

TL;DR: This mechanism promotes that proteins become immobilized by the area/s where the highest lysine residues density is located and explain the high enzyme stabilization usually achieved by using this immobilization technique.

TL;DR: Decolourisation of the recalcitrant dye Remazol Brilliant Blue R by the fungus basidiomycete Pleurotus ostreatus was investigated and treatment with the laccase mixture reduced its toxicity by 95%.

TL;DR: In this paper, a new strain of Bacillus sp. I-3, isolated from natural soil samples, showed a high raw starch digesting activity towards potato starch and reached 642 U/mL.

TL;DR: No plasmids were recorded for strains ST28MS and ST26MS, suggesting that the genes encoding production of the two bacteriocins are located on the genomes.

TL;DR: The lipases from Thermomyces lanuginosus and Candida antarctica B were comparatively assayed for the synthesis of sugar esters by transesterification of sugars with fatty acid vinyl esters in 2-methyl-2-butanol:dimethylsulfoxide mixtures and it was found that lipase from C. antarctic B is particularly useful for the preparation of 6,6′-di-acylsucrose.

TL;DR: The ability of a cellulase preparation to hydrolyze pretreated softwood showed little correlation with its activity on filter paper, carboxymethylcellulose and Avicel; however, there was a significant correlation with the level of endogenous β-glucosidase and xylanase activity.

TL;DR: Lipase stability in the presence of organic solvents, as well as in acidic and alkaline pHs and at high temperatures makes it a good candidate for its application in non-aqueous biocatalysis.

TL;DR: Nine zygomycetes strains including three strains of Rhizopus oryzae, Mucor corticolous, M. hiemalis and M. indicus showed greater ethanol production than the other strains, and the strains also rapidly consumed hydroxymethyl furfural present in DAH.

TL;DR: The aim of the present work was to increase the fibrous content in SSF of steam pretreated spruce to 10% by adapting the yeast to the inhibitory substrate and by using a fed-batch process.

TL;DR: The effect of different reaction parameters was explored on the synthesis of isoamyl acetate by enzymatic catalysis in n-hexane and the impact of the acyl donor was investigated, among several reactants, acetic anhydride gave best yields.

TL;DR: The rapidly expanding potential of biomolecular engineering techniques has created the exciting potential of remediating some of the most recalcitrant and hazardous compounds in the environment.

TL;DR: In this paper, different cellulosic materials were treated with different extraceller microbial enzymes and the variation of crystal structure was found to attack preferentially the (0.0.2) crystal planes for cellulose I during enzymatic hydrolysis.

TL;DR: In this article, a factorial design and response surface analysis was used to optimize the production of inulinase (2,1-β-d -fructan fructanohydrolase, EC 3.2.7) by Kluyveromyces marxianus ATCC 16045, using sucrose as carbon source.

TL;DR: The lack of correlation between initial cellulose concentration and final filter paper activity in the medium could therefore be assigned to adsorption of a fraction of the cellulases produced by P. brasilianum IBT 20888 onto remaining cellulose.

TL;DR: In this article, the authors used Response Surface Methodology (RSM) based on a five-level, four-variable central composite rotatable design (CCRD) to evaluate the interactive effects of synthesis, of reaction time (2.5-10 h), temperature (30-70 °C), amount of enzyme (0.1-0.2 g) and substrate molar ratio (palm oil to oleyl alcohol, 1:1-1:5) on the percentage yield of wax esters.

TL;DR: The treatment of OOMW with the lacc enzyme showed similar results to those reported with the fungus indicating that laccase plays an important role in the degradative process, suggesting that it could be a useful tool for this and other environmental applications.

TL;DR: In this article, the authors compared the thermal and pH stabilities of free and immobilized α-amylase, β-, and glucoamylases, in which immobilization support was prepared by equal weights of chitosan and activated clay and were cross-linked with glutaraldehyde.

TL;DR: Bacillus sp.

TL;DR: The N-terminal amino acid sequences of four laccase and two MnP isoenzymes resembled those of the two other selective white-rot fungi applied in biopulping, i.e. Ceriporiopsis subvermispora CZ-3 and the strain IZU-154.

TL;DR: Development of a process involving an enzymatic (specific) degradation of caffeine to non-toxic compound is necessary to solve the problems of chemical extraction of caffeine in food products as well as treating the caffeine containing waste products.

TL;DR: In this article, the effect of lactose concentration and oxygen level on the growth and metabolism of K. marxianus UFV-3 in cheese whey permeate was investigated.

TL;DR: In this article, a cellulase production by Streptomyces drozdowiczii, a new cellulolytic species isolated in Brazil from forest soil, using agro-industrial by-products was also assessed.

TL;DR: Close contact between the adsorbed lipase and the immobilized lipase permitted to alter the catalytic and functional properties of this lipase, which can open new routes for the modulation of lipase properties.

TL;DR: There was an optimum impeller speed in maximizing the population of Cel+ cells and BC production and the by-product, water-soluble polysaccharide (WSPS), was a homopolysaccharides of glucuronic acid.

TL;DR: In this article, Aspergillus flavipes BICC 5174 was used for the production of lovastatin by solid state fermentation (SSF) using different solid substrates such as wheat bran, bagasse, barley, soybean meal, gram bran and fruits waste and some of their combinations were tested for Lovastatin production.

TL;DR: The results obtained indicate that AO7 was only removed in the presence of active biomass, the removal rates being higher in the absence of a cosubstrate, and the improvement of mass transfer conditions in the medium as a consequence of the high biogas production is also a key topic.

TL;DR: In this paper, the individual and combined efficiencies of four selected, commercial, multicomponent enzyme preparations Celluclast 1.5 L (from Trichoderma reesei ), Finizym (from Aspergillus niger ), Ultraflo L(from Humicola insolens ), and Viscozyme L (aspergilla aculeatus ) in catalyzing arabinose and xylose release from water-soluble wheat arabinoxylan in an industrial fermentation residue (still bottoms) in lab scale experiments were examined.

TL;DR: Strong evidence of cyanide biodegradation and co-metabolism emerged from studies with flask cultures where glucose was provided as a co-substrate and growth yield calculations performed provided values that were comparable to those reported in literature for one-carbon substrates.