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Showing papers in "Enzyme and Microbial Technology in 2016"


Journal ArticleDOI
TL;DR: The current knowledge available about green synthesized nanoparticles with efficacy against mosquito-borne diseases and cancer is focused on, with special reference to the chemical standardization of the botanical extracts used for nanosynthesis and the potential effects on green fabricated nanoparticles on non-target organisms.

220 citations



Journal ArticleDOI
TL;DR: The synthesis, characterization, development and applications of organic-inorganic hybrid nanoflowers formed of various enzymes and metal ions are discussed and potential mechanism underlying enhanced catalytic activity and stability is explained.

177 citations


Journal ArticleDOI
TL;DR: This review discusses the methods of enzymatic functionalization of four well-known polysaccharides, chitosan, cellulose, pectin and starch, and the enzymes implicated in the modification such as laccases, peroxidases lipases, tyrosinases, and transglutaminases.

146 citations


Journal ArticleDOI
TL;DR: The present study highlights the microbial synthesis of silver and gold nanoparticles by Sporosarcina koreensis DC4 strain, in an efficient way, and shows that 3μg concentration of silver nanoparticles sufficiently enhance the antimicrobial efficacy of commercial antibiotics against pathogenic microorganism.

140 citations


Journal ArticleDOI
TL;DR: Analysis of flavonoid methoxides including O- and C-methoxide derivatives in aspect of structure, bioactivities and description of almost all up-to-date O-and C-methyltransferases' enzymatic characteristics are concentrated on.

127 citations


Journal ArticleDOI
TL;DR: Under optimal experimental conditions, reusability tests of the biocatalyst (TLL-PMA) after thirty successive cycles of reaction were performed and the nature of the product (isoamyl oleate) was confirmed by attenuated total reflection Fourier transform infrared (ATR-FTIR), proton ((1)H NMR) and carbon ((13)C N MR) nuclear magnetic resonance spectroscopy analyses.

122 citations


Journal ArticleDOI
TL;DR: This green synthesis of gold nanoparticles (AuNps) is cost-effective and advantageous for the development of a new class of probiotics mediated and non-toxic carriers in targeted drug delivery systems, cancer diagnostic, photothermal therapy, biosensing, and medical imaging.

115 citations


Journal ArticleDOI
TL;DR: The present review is designed to speculate the importance of usage of vast marine resources and its mediated NPs synthesis, in particular seaweed-mediated NPS synthesis to overcome the limitations involved in physical and chemical methods.

106 citations


Journal ArticleDOI
TL;DR: The flower shaped-hybrid nanomaterials called hybrid nanoflower (HNF) consisting of urease enzyme and copper ions (Cu(2+)) are synthesized and a mechanistic elucidation of enhancement in both activity and stability of the HNF is reported.

101 citations


Journal ArticleDOI
TL;DR: This work illustrates the preparation of magnetic macromolecular glucoamylase CLEAs using dialdehydic pectin, as a cross linker instead of traditional glutaraldehyde, which is 22% higher than traditional magnetic CLEAs.

Journal ArticleDOI
TL;DR: The developed biosensor using a glassy carbon electrode modified with gold nanoparticles and tyrosinase within a dihexadecylphosphate film showed good repeatability and stability and was successfully applied in the determination of catechol in natural water samples.

Journal ArticleDOI
TL;DR: The PA6/CHIT nanofiber matrix used in this study not only proved to be a suitable carrier for immobilized and modified laccase but was also efficient in the removal of a mixture of endocrine disrupting chemicals in three treatment cycles.

Journal ArticleDOI
TL;DR: Proteomic analyses and enzymology studies are combined to show that sequential and submerged cultivation methods differently influence both titers and secretion profile of key enzymes required for the hydrolysis of sugarcane bagasse.

Journal ArticleDOI
TL;DR: These NPs showed enhanced antimicrobial, antioxidant, and anticancer activities, though it is more pronounced for Au-Ag alloy NPs, which is due to the combining effect of phytochemicals, Au and Ag metals, and could be applied as effective growth inhibitors for various biomedical applications.

Journal ArticleDOI
TL;DR: Different modified Novozym 435 preparations, differing in their surface nature and in their interactions with reactants, have been compared in the alcoholysis of Camelina sativa oil and three derivatizations studied make the matrix particles more resistant to rupture.

Journal ArticleDOI
TL;DR: This mini-review will examine the current best practices and understanding of nanoparticle (NP) formation kinetics and mechanisms to establish a path forward for this technology.

Journal ArticleDOI
TL;DR: There is a need to expand the sequence database in order to strengthen the link between AcXE gene sequence and specificity, and the functional metagenomics approach is still hampered by screening bottlenecks.

Journal ArticleDOI
TL;DR: Among the various beta and gamma amino acids produced via asymmetric synthesis, (S)-4-amino-4-(4-methoxyphenyl)-butanoic acid showed highest analytical yield (82%) with excellent enantiomeric excess (>99%).

Journal ArticleDOI
TL;DR: There is moderate evidence that acupuncture relieves pain better than some sham treatments, measured at the end of the treatment, and this is maintained at short-term follow-up.

Journal ArticleDOI
TL;DR: A rapid, ecofriendly method of fabricating stable gold nanoparticles (AuNPs) using latex of Jatropha curcas is reported for the first time and will bring a new research direction to explore eco-friendly nanoparticle in insect control via inhibition of enzyme catalytic potential.

Journal ArticleDOI
TL;DR: The results showed Klebsiella pneumoniae and Xanthomonas campestris to be more sensitive among the test human pathogen and phyto-pathogen respectively and synergistic effect of silver nanoparticles in combination with kanamycin which displayed increased fold activity up to 58.3% against Klebsia pneumoniae.

Journal ArticleDOI
TL;DR: The intracellular synthesis of SeNPs by the moderate halophilic bacterium, Idiomarina sp.

Journal ArticleDOI
TL;DR: A compilation of liver injury cases establishes causality for 28/57 different TCM herbs and herbal mixtures, aiding diagnosis for physicians who care for patients with liver disease possibly related to herbal TCM.

Journal ArticleDOI
TL;DR: It was confirmed that the conducting polymer polypyrrole was formed within periplasmic space and/or within the cell wall of yeast cells, which were incubated in solution containing pyrrole, glucose and [Fe(CN)6](4-).

Journal ArticleDOI
TL;DR: The study of synergistic effects between elicitor, aw and pH on taxol production showed that the highest yield of taxol was produced under modified ionic stress of 0.98aw (KCl) at pH 5 when supplemented with 20mg/l of salicylic acid.

Journal ArticleDOI
TL;DR: The extraction of cell surface proteins from R. oryzae using common detergents and reducing agent and their effect on the size and shape of the biosynthetic AuNPs are explored, finding them suitable for biomedical applications.

Journal ArticleDOI
TL;DR: The biosynthesis of CdS QDs by acidophilic bacteria of the Acidithiobacillus genus is reported for the first time, marking the first report of the generation of QDs with improved properties by using extremophile microorganisms.

Journal ArticleDOI
TL;DR: The results indicate that using protein modeling program and creating mutation, can enhance lipase activity and/or thermostability of SML and it also could be used for improving other properties of enzyme to the desired requirements as well as further mutations.

Journal ArticleDOI
TL;DR: The results of this study showed that the fluorescence signals generated in samples using specific substrate molecules can be utilized to develop a bio-sensing platform for the detection of E. coli in drinking water.