Showing papers in "Enzyme and Microbial Technology in 2018"

TL;DR: The present biosynthesis approach is rapid, inexpensive and eco-friendly and it yielded highly stable ZnO NPs with significant antioxidant and anticancer potential for the treatment of lung cancer and subsequent therapeutic applications.

TL;DR: The enzyme under lower-intensity ultrasonic irradiation leads to favorable conformational changes, thereby enhancing its activity, and this highly activated lipase was encapsulated within zeolite imidazolate framework-8 as a metal-organic framework (MOF) material via facile one-step biomineralization method.

TL;DR: The new biocatalysts are significantly more stable and avoid enzyme subunit desorption, being the immobilization pH a key point in their design.

TL;DR: The research results have high impact for the utilization of Kraft lignin as nanosized colloidal particles in advanced bionanomaterial applications in medicine, foods and cosmetics including different sectors from chemical industry.

TL;DR: Rec recombinant P6281 (rP6281) expressed in Pichia pastoris significantly inhibited the development of grey mold that causes rotting of apple, orange, and cucumber, indicating that rP 6281 may be developed as an effective anti-mold agent for fruit storage.

TL;DR: Combination of dark and photofermentation resulted in the maximal H2 yield of 17.6L/L of distillery waste with chemical oxygen demand 40g/L, equivalent to 205kJ/L distillery wastewater and corresponds to recovery of approximately 4-8% of energy consumed during ethanol production.

TL;DR: Einleitung „Slow Food“ ist eine weltweite Bewegung von Menschen, die sich aus Überzeugung and Leidenschaft f for gutes, sauberes and faires Essen einsetzen.

TL;DR: Proteomics approaches that have been applied to the study of fungal secretomes are discussed, focusing on two of the most studied filamentous fungi genera: Trichoderma and Aspergillus.

TL;DR: This paper identifies enzymes that are phenol tolerant, and whose adoption for lignocellulose hydrolysis could contribute to reductions in enzyme loading needed to hydrolyze alkali pretreated lignOcellulosic substrates in the presence of lignin derived phenols.

TL;DR: A Box-Behnken design (BBD) optimization process to synthesize eugenyl benzoate by esterification of eugenol and benzoic acid catalyzed by the chitosan-chitin nanowhiskers supported Rhizomucor miehei lipase (RML-CS/CNWs) overcomes the well-known drawbacks of the chemical route in favor of a more sustainable reaction process.

TL;DR: The introduction of a three-enzyme cascade for the production of oligo-ε-caprolactone provided self-sufficiency with respect to NADPH-cofactor regeneration and reduced inhibiting effects on the central CHMO enzyme.

TL;DR: This study describes the use of chemically functionalized CB Vulcan XC 72R for the development of sensitive electrochemical biosensors and constructed a Tyrosinase (Tyr) biosensor based onfunctionalized CB and dihexadecylphosphate (DHP) for the determination of catechol in water samples.

TL;DR: The biosensor measured sarcosine level in sera of prostate cancer patients, which was significantly higher than those of apparently healthy persons (p value <0.01), and a linear relationship was obtained between sarcosines concentration in the range, 0.1-100 μM and current (mA) under optimum conditions.

TL;DR: The data suggest that electron shuttling by mediators is not a significant general mechanism for enhancing laccase catalyzed oxidation of biorefinery lignin substrates, and the results thus provide a new view on laccases catalyzed lign in modification.

TL;DR: Results showed that not only the position 501 is significant for functional expression of B. pumilus W3 CotA, but also these mutants still remained its high thermostability, resistance of alkaline with salt, and conspicuous decolorizing efficiency.

TL;DR: It is reported that recombinant manganese peroxidase (rMnP) produced from the yeast Pichia pastoris can degrade furfural and HMF making them less toxic to microorganisms, providing an avenue to use rMNP to increase the growth of fermentation microorganisms that are inhibited by toxic compounds derived from pretreatment of biomass.

TL;DR: A direct approach is presented to estimate the OD at 578 nm (OD578) of bacterial cultures in microplates without the need for sample dilution, and it is shown that just a few OD calibration points are required to adjust this conversion formula and make it suitable for other suspensions or cultures of bacterial strains different than P. putida.

TL;DR: The optimized fermentation produced 42g/l rhamnolipids at a yield of approximately 47% and a volumetric productivity of 220mg/l-h, which are among the highest reported.

TL;DR: Structural differences between the two enzymes are exploited with the aim of engineering BbAfcB into a more efficient transfucosidase and an understanding of structure-function relations of hydrolytic activity vs. transfucOSylation activity is approached.

TL;DR: Shewanella oneidensis MR-1, a facultative aerobic bacterium that has been extensively studied for its utility as biofuel cells as well as for the detoxification of heavy metal oxides, has been applied for the first time as a whole-cell biocatalyst for formic acid synthesis from gaseous CO2 and electrons supplied from an electrode.

TL;DR: A novel pathway to produce inositol from glucose by a trienzymatic cascade system involving polyphosphate glucokinase (PPGK),Inositol 1-phosphates synthase (IPS) and inositl monophosphatase (IMP) is reported, which is promising for the enzymatic synthesis of inositols without ATP supplementation.

TL;DR: Characterization and structural analysis demonstrated that directed evolution and site-directed mutagenesis could produce cumulative or synergistic improvements in thermostability or catalytic efficiency of phytase.

TL;DR: This study reported that a marine Bacillus sp.

TL;DR: In this article, error-prone PCR and site-directed mutagenesis were utilized to improve the thermostability of nitrilases, and several mutants (AcN-Q339K, AcNQ343K and AcN-T201F) were obtained with dramatically improved temperature.

TL;DR: In the present study, two amino acids were perceived to mark the temperature dependability of xylanase from Geobacillus thermodenitrificans C5, which resulted into enhancement in their thermostability.

TL;DR: Physical immobilization by hydrophobic interactions was performed with three well-characterized cutinases using Lewatit VP OC 1600 as the macroporous support and the results herein set the stage for the in-depth evaluation of these catalysts for selective transformations in organic media.

TL;DR: The comparison showed that the polymerization of dopamine was greatly improved when catalyzed by laccase, and ether bonds were found between dopamine units of PDA; while such bonds were not observed in film obtained from the conventional method.

TL;DR: The results suggested that an exchange of the metal ion can be used for tailoring the properties of PepA for specific hydrolysis requirements.

TL;DR: Results indicate that, rather than working alone, CalXyn11A has an important role in enhancing total lignocellulose degradation activity by cooperation with other GHs.

TL;DR: Bacteria present in wastewater are electroactive microbial species which can donate electron to an electrode using conductive appendages or nanowires, while consuming the organic matter present in the wastewater, which shows microbial metabolism for water treatment and generate electricity.