Showing papers in "Enzyme and Microbial Technology in 2021"

TL;DR: The superior activity and thermostability of BhrPETase rendered it one of the most promising PETases for plastic waste recycling and bioremediation applications in the future.

TL;DR: The suitability of postbiotics to combat microorganisms that deal with food safety should be tested mainly by analyzing the chemical composition and conducting antagonistic tests.

TL;DR: This is the first report exploring the feasibility of endophytic fungi for epothilone producing potency, that could be a novel platform for industrial production of epothILone.

TL;DR: In this article, a recent progress in the discovery, characterization, and enzymatic mechanism elucidation of polyethylene terephthalate (PET) hydrolases is reviewed.

TL;DR: In this article, the lipase from Rhizomucor miehei (RML) was covalently immobilized on different supports, two silica gels and two carbon nanotube samples, using two different strategies.

TL;DR: Details on the aspects of microbial (bacterial and fungal) dem methylation of lignins and lignin-model compounds are discussed and evidence of enzymes identified as specific O-demethylases involved in demethylation is provided.

TL;DR: This work investigated the use of redox-active polymers based on bovine serum albumin and chitosan, covalently bound to mediators neutral red and ferrocene and containing carbon nanotubes, for immobilization of Paracoccus yeei VKM B-3302 bacteria.

TL;DR: The results from fluorescence microscopy and flow cytometric assay suggest that the β-CD-CUR/MAase system exhibited higher cytotoxic and apoptotic effects on cancer cells compared to other formulations.

TL;DR: The substituted residues located in the "palm" region of the N-terminus and the newly generated hydrogen bonds in the mutants might contribute to improved thermostability.

TL;DR: In this article, the constitutive promoter J23100 driven lysine decarboxylase (CadA) for cadaverine production was established and compared in different Escherichia coli strains.

TL;DR: In this article, a review of the use of hydrogen peroxide in biocatalysis using enzymes, bioelectrocatalyis, photocatalysis, and cold temperature plasmas is presented.

TL;DR: This review highlights the potential of lactobacilli to release functional enzymes associated with the digestive process and how this complex metabolism can be explored to contribute to the human diet.

TL;DR: In this article, the authors reported antifungal activity of ZV-AgNPs against Colletotrichum falcatum Went for the first time using Bacillus sp. strain AW1-2, which was identified through 16S rRNA gene sequence analysis.

TL;DR: A comparison of enzyme encapsulation based on different principles with a focus on materials inspired by nature is presented in this article, where the authors provide a comparison of enzymes encapsulated based on these different principles.

TL;DR: In this paper, a novel and efficient QQ-PAC core-shell beads were prepared for mitigating the membrane contamination, which was composed of two parts: QQ bacteria embedded in the core and PAC in the shell.

TL;DR: The results confirm that Bacillus sp.

TL;DR: Wang et al. as discussed by the authors developed an efficient expression system for active protein glutaminase (PG) in Bacillus subtilis using combinatorial optimization involving promoters, signal peptides, and culture medium.

TL;DR: In this article, the 3-aminophenylboronic acid (3-APBA) was used as a hydrophilic ligand for phase transfer of oleylamine (OA) capped Au NPs (OA@Au NPs) from non-hydrolytic system into aqueous solutions.

TL;DR: In this paper, computational screening of an extreme temperature aquatic habitat metagenomic resource was done, leading to the identification of a novel gene, bglM, encoding a β-glucosidase.

TL;DR: In this paper, the binding of three PET hydrolases on a suspended PET powder under conditions of both enzyme-and substrate excess was studied, and the saturated substrate coverage approximately corresponded to a monolayer on the PET surface.

TL;DR: In this article, a chitinolytic bacterial community (CBC) was identified that showed high chitin degradation activity under thermophilic conditions, i.e., 1% (w/v)-chitin powder degraded completely within 7 days at 60 °C.

TL;DR: In this paper, an Efeb gene of 1251bp encoding DyP-type peroxidase from Bacillus sp. strain BL5 (DyPBL5) was amplified, cloned into a pET-28a (+) vector and expressed in Escherichia coli BL21 (DE3) cells.

TL;DR: This work enhances the understanding of the structure-function relationship of PRO and provides an academic foundation for improving the enzymatic properties of PRO to satisfy industrial requirements using protein engineering.

TL;DR: In this article, a simple and efficient enzyme immobilization method through phytic acid (PA) modified α-Glucosidase (α-Glu)/Cu3(PO4)2·3H2O hybrid nanoflower was developed.

TL;DR: An easy and fast protocol for cloning, expression, and purification of active LPMOs in the following architecture: natural signal peptide, LPMO enzyme, TEV protease site, and His6-Tag is provided.

TL;DR: In this paper, the free PTE was hybridized with copper ions (Cu2+) to enhance its catalytic stability and activity, and the obtained particles were freeze-dried after precipitation with PO43- at 4 °C for 72 h.

TL;DR: The protein ligand docking and molecular dynamic simulation results showed that C- terminus region of endolysin play vital role in cell wall binding and even in the absence of holin, hydrolyze a broad range of gram negative bacterial pathogens.

TL;DR: It was concluded that the transcriptional activator Msn2, the HOG1 signaling pathway and the cAMP-PKA signaling pathway were involved in the regulation of liamocin biosynthesis and production.

TL;DR: Yeast microbial fuel cells established as biosensors for in-situ monitoring of dissolved oxygen (DO) levels in environmental waters, with yeast and glucose substrates acting as biocatalyst and fuel, respectively demonstrate promising prospects for future monitoring in a variety of areas, including developing countries and remote locations.

TL;DR: In this paper, a high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) analysis demonstrated that Aspergillus fumigatus LPMOs are able to oxide cellulose at C1 and C1/C4 positions.