Showing papers in "Enzyme and Microbial Technology in 2022"

TL;DR: Current progress in modifying enzyme immobilized biosensors to detect biologically essential molecules (glucose, urea, cholesterol) is covered and a discussion of the different types of enzyme immobilization is provided.

TL;DR: In this paper , a ligninolytic enzyme-producing strain F5 was identified as Bacillus thuringiensis, which can efficiently degrade methylene blue (MB) dye.

TL;DR: In this article , the decyl esterification with decanol in solvent-free systems was performed using lipase from Thermomyces lanuginosus (TLL) immobilized by physical adsorption on silica particles extracted from rice husk, an agricultural waste.

TL;DR: In this article , a detailed review of potential and relevance of plant chitinases for controlling pathogens infection in plant and prospecting to improve plant defense, growth and yield is presented.

TL;DR: In this paper , a GH78 α-L-rhamnosidase AtRha from Aspergillus terreus CCF3059 was screened and expressed in Pichia pastoris KM71H.

TL;DR: In this article, a GH78 α-L -rhamnosidase AtRha from Aspergillus terreus CCF3059 was screened and expressed in Pichia pastoris KM71H.

TL;DR: In this article, a β-glucosidase from Penicillium decumbens was used to convert ginsenoside Rb1 to C-K using a compound K substrate.

TL;DR: In this paper , ancestral sequence reconstruction (ASR) was employed to identify the natural evolutionary trends within 5 Å around the catalytic center of TrLip and two mutation libraries were constructed.

TL;DR: In this paper , the authors presented a novel approach for switching cofactor specificity of glutamate dehydrogenase RocG from nicotinamide adenine dinucleotide phosphate (NADPH) to n-acetyl-naphacetylnaphosphate (nADH) to improve poly-γ-glutamic acid (γ-PGA) production.

TL;DR: In this article , a flexible enzymatic glucose biosensor was prepared by electrochemical deposition of dendritic gold nanostructures on carbon cloth substrate, then covalently immobilizing glucose oxidase on the nanostructure, and then increasing enzyme loading by enzyme precipitation coating.

TL;DR: In this paper , the pyridoxal kinase (PdxY) was introduced in the whole-cell system to construct a new GABA producing system, and 100% conversion of 0.6 M MSG was obtained.

TL;DR: In this article, a β-1,4-Galactosyltransferase was identified from Aggregatibacter actinomycetemcomitans and was easily overexpressed in E. coli in soluble form.

TL;DR: In this article , a β-1,4-Galactosyltransferase was identified from Aggregatibacter actinomycetemcomitans, which was easily overexpressed in E. coli in soluble form.

TL;DR: Wang et al. as mentioned in this paper fused Dermaseptin SI (DSI) and fused it onto the N-terminus of Thermobifida fusca cutinase mutant D204C/E253C (Tfuc2), and achieved the PET degradation rate up to 57.9% at 70 °C for 96 h, which was 22.7-fold of that of Tfuc 2 itself.

TL;DR: In this paper , a novel heterologous protein expression system with remarkable reduction of undesired background proteins was developed by deletion of the p53-like transcriptional factor Vib1.

TL;DR: In this article , a compartmentalized UV absorbance assay for continuous detection of soluble hydrolysis products released during enzymatic degradation of poly(ethylene terephthalate) (PET) was presented.

TL;DR: In this article , composite polyacrylonitrile -multiwall carbon nanotubes nanofibers (PAN-MWCNTs NFs) were used for glucose detection.

TL;DR: In this article , the authors described the metabolic engineering of Escherichia coli Nissle 1917 (EcN) to enhance heparosan production by optimizing the biosynthesis of precursors UDP-GlcA and UDP-glcNAc and the expression of heparusan synthase.

TL;DR: In this article , the authors mine and identify the carotenoid biosynthesis genes in Schizochytrium sp. They obtained the gene sequences of a trifunctional enzyme (CrtIBY), carotene hydroxylase (CrTZ), and Carotene ketolase(CrtO) in carotensynthesis pathway by bioinformatics analysis.

TL;DR: In this paper , a polyvinyl alcohol/sodium alginate (PVA/SA@Fe3O4) magnetic immobilized-enzyme hydrogel beads were prepared and their structure and morphology were characterized by scanning electron microscopy, surface area and porosity analyses.

TL;DR: In this paper , the Saccharomyces cerevisiae native precursor pathways were explored and higher-active CHSs from plants rich in flavonoids were screened, and the results indicated that over-expressing the native precursor pathway is not an efficient approach to improving (2 S)-naringenin production in our chassis strain.

TL;DR: In this article , a quick and disposable electrochemical biosensor based on bovine hemoglobin (BHb) is proposed for the determination of chlorpyrifos (CP).

TL;DR: In this paper , an antimicrobial peptide derived from the venom of the wasp Synoeca surinama was used as a biorecognition element tethered over chitosan-coated magnetic nanoparticles (Fe3O4-Chit).

TL;DR: In this paper , enzymatically pre-treated sugarcane bagasse (SCB) was used as substrate for sequential production of hydrogen (H2) and methane (CH4), while the metabolites accumulated at the end of this stage (9140.5 mg HAc/L) were used by allochthonous inoculum for CH4 production.

TL;DR: In this paper , a chromogenic growth and isolation solid medium based on MnO2 was developed to selectively allow the growth of exoelectrogenic bacteria and change the medium color in the process.

TL;DR: In this paper , full-length chitin-acting enzymes (ChiA, ChiB, ChiC, and CBP21) of Serratia marcescens BWL1001 and CBM-truncated versions of ChiB and ChiC were heterologously expressed for enzymological analysis.

TL;DR: In this paper , proteins regulating their production and activity were explored in Lactobacillus species with the help of STRING (11.5) as a bioinformatic tool.

TL;DR: In this paper , the white-rot basidiomycete Phanerochaete chrysosporium (PcSTSs) were isolated as full-length cDNAs encoding a mature open reading frame.

TL;DR: A dual-enzyme screening method was developed to measure the activity of the D-allulose 3-epimerase from Clostridium cellulolyticum H10 by employing a xylose isomerase, enabling high-throughput screening of mutants with higher thermostability as discussed by the authors .

TL;DR: In this article , a new Oxd (protein gb|EEU37245.1 named OxdFv) from Fusarium vanettenii 77−13−4.