Showing papers in "Experimental Cell Research in 1988"

TL;DR: Human lymphocytes were exposed to X-irradiation or treated with H2O2 and the extent of DNA migration was measured using a single-cell microgel electrophoresis technique under alkaline conditions and this technique appears to be sensitive and useful for detecting damage and repair in single cells.

TL;DR: Osteoclasts and activated macrophages in culture were shown to generate an acidic microenvironment specifically in the attachment zone between the cell and the base of the culture dish, which would favor the direct extracellular action of secreted lysosomal proteinases in the degradation of collagen by both cell types in vivo.

TL;DR: The use of LY in a rapid and efficient test for the determination of the best electrical-shock conditions of other cell lines is described, which can reach a value equivalent to the extracellular concentration and can be regulated by controlling the external concentration.

TL;DR: It is demonstrated that an optical microlithography technique used in the semiconductor industry can be used to position cells with a precision of less than a micron and both positive and negative cytoscripts of photoengraved images can be obtained.

TL;DR: Data support the idea that multidrug resistance is based on active nonspecific efflux of the drugs and indicate that the simple procedure of cell staining can be used for the detection of resistant cells and further study of the phenomenon of multidrog resistance.

TL;DR: The data suggest a specific reprogramming of various cellular activities in response to contact with the reconstituted extracellular matrix.

TL;DR: Results suggest that the cells arrested with TSA are quiescent (G0), and the removal of TSA induced a rapid transient increase in the transcription of c-fos and the cells required 15 h to enter the S phase after release.

TL;DR: Repeated DNAs from the constitutive heterochromatin of human chromosomes 1 and 18 were used as probes in nonradioactive in situ hybridization experiments to define specific numerical and structural chromosome aberrations in three human glioma cell lines and one neuroblastoma cell line.

TL;DR: It is reported that the induction of circular ruffle arrangements is an effect specific for PDGF, dose-dependent and inhibitable by anti-PDGF antibodies, and a shift in the time-course of the PDGF-induced circular ruffling formations is found by sodium orthovanadate, an inhibitor of protein tyrosine phosphatases.

TL;DR: In this article, the fluorescence-activated cell sorter was used for the purification of human myoblasts using the specific reaction of monoclonal antibody 5.1H11 with a human muscle cell surface antigen.

TL;DR: Flow cytometric analysis of BrdU-quenched 33258 Hoechst fluorescence may be used to measure cell activation and the G1, S, and G2/M compartment distributions in each of three successive cell cycles after growth stimulation of human peripheral blood lymphocytes.

TL;DR: The results indicate that the only function of transferrin in supporting cell proliferation is to supply cells with iron, as demonstrated by blocking monoclonal antibodies against transferrin receptors.

TL;DR: The results indicate that WGA inhibits the uptake of karyophilic proteins in general and support the concept that the O-glycosidically bound carbohydrates of certain nuclear pore complex proteins are exposed to the pore interior and that these regions are probably involved in nucleocytoplasmic translocation processes.

TL;DR: It is shown that neomycin, a drug which has a high affinity for phosphoinositides and in vivo interferes with the PI cycle, inhibits the polymerization of actin in platelets induced either by thrombin or by ADP.

TL;DR: Data from in vivo and in vitro endogenous protein phosphorylation experiments suggest that 6-DMAP may directly or indirectly affect the activity of a relevant c-AMP and Ca2+-independent protein kinase which is stimulated after hormone addition and seems to support starfish oocyte maturation.

TL;DR: It is suggested that Kupffer cells in liver lobules and macrophages in the blood may play important roles, mainly via IL-1, in repair of liver damage and regeneration.

TL;DR: Fertilized sea urchin eggs undergo a series of rapid and synchronized mitotic divisions, and the transient activation of HH1K and the intracellular mitotic factors driving the cell cycle is discussed.

TL;DR: It is shown in vitro by immunohistochemistry, catalytic histochemistry, and by affinity chromatography of solubilized plasma membrane on collagen-Sepharose that DPP IV has a binding affinity to collagen, and this binding does not affect the activity of DPPIV.

TL;DR: It is shown that HSV lytic infection results in an accumulation of at least two cell-encoded polypeptides, one of which is related to hsp90.

TL;DR: Fibroblasts and chondrocytes that had been passaged to stimulate dedifferentiation did not incorporate more 35SO4 when treated with cytochalasin D, suggesting that increased proteoglycan synthesis in response to rounding may itself be a differentiated property of chondROcytes.

TL;DR: The immunolocalization data suggest that 7-2-ribonucleoprotein may be involved in stages of ribosome biogenesis which take place in the granular component of the nucleolus, i.e., assembly, maturation, and/or transport of preribosomes.

TL;DR: A strong coupling of p11 and p36 in cellular compartmentalization and tissue differentiation is indicated and coincident expression patterns of both proteins confirming and extending previous results with p36 antibodies are shown.

TL;DR: It is found that bovine adrenal capillary endothelial cells have specific receptors for human urinary-type plasminogen activator on the cell membrane, and both the native molecule and the A chain are able to stimulate endothelial cell motility in the Boyden chamber, when used at nanomolar concentrations.

TL;DR: The induction of dendritic processes in melanocytes by dibutyryl cAMP or sodium butyrate was reversible and appears to reflect the expression of the mature melanocytic phenotype in situ.

TL;DR: Under the experimental conditions described, phagocytosis of HK-yeast by murine macrophages is mediated by and large by the beta-glucan receptors, while the mannose receptors and complement receptors do not contribute to the process.

TL;DR: Hepatocytes of transgenic mouse fetuses harboring SV40 virus transforming gene sequences in the SV delta e-MGH fusion gene construct 202 driven by the mouse metallothionein (MT-I) enhancer appear to be present in the liver of the transgenic mice, indicating immortalized, nonmalignant phenotype.

TL;DR: It is suggested that EGF remains associated with receptors during endocytosis in A431 cells until it is transferred to lysosomes where the pH of the EGF microenvironment is dropped to 5.2, suggesting a prolonged presence of EGF-receptor complexes in the para-Golgi region might be of importance in mitotic signaling.

TL;DR: The minimum sequence requirements for efficient nuclear import of PE conjugates were found to be similar to those previously defined genetically for large T antigen itself, and no competitive inhibition of uptake was observed in cells expressing nuclear or cytoplasmic T antigen.

TL;DR: The observation establish an unique biological effect associated with the period of elevated synthesis of the HSP, especially the hsp 70, which is found in heat-resistant variants of HA-1 cells that express elevated levels of this protein.

TL;DR: The K-3 monoclonal antibody (MAb) is employed which specifically binds to the hyaluronate receptor and blocks its ability to interact with hyAluronate.