Showing papers in "Experimental Cell Research in 2003"

TL;DR: The structure and function of the EGFR is reviewed, from ligand binding to the initiation of intracellular signalling pathways that lead to changes in the biochemical state of the cell.

TL;DR: The neuregulins (NRGs) are cell-cell signaling proteins that are ligands for receptor tyrosine kinases of the ErbB family that play essential roles in the nervous system, heart, and breast.

TL;DR: The rapid local capture of TGFα by the EGFR has fundamental biological importance and is a phenomenon that is critical in diverse biological processes such as vulva development in the nematode Caenorhabditis elegans (C. elegans) and hair follicle organization in mammals.

TL;DR: Necrosis appears to be a specific form of execution phase of programmed cell death, and there are several examples of necrosis during embryogenesis, a normal tissue renewal, and immune response.

TL;DR: This review will focus on receptor cooperativity, i.e., ErbB1 cooperates with other classes of receptors, and the ligand-less ErBB2 functions as a heterodimer with other ErbBs.

TL;DR: This review highlights recent structural insights into the mechanism of ligand-induced heterodimer formation, and concentrates on signaling pathways employed by ErBB-2 and ErbB-3 in normal and in malignant cells.

TL;DR: Results indicate that, while cadmium is an effective inhibitor of DNA MeTase and initially induces DNA hypomethylation, prolonged exposure results in DNA hypermethylation and enhanced DNA Me tase activity.

TL;DR: Microarray was used to probe for consistent gene changes in response to lithographically produced topography with time and showed many initial gene changes and also down-regulation of gene response with time.

TL;DR: Overexpression of ErbB family members can disrupt normal receptor trafficking by driving heterodimerization of receptors with disparate trafficking patterns, and disrupted trafficking could be an important factor in the altered signaling patterns observed as a consequence of receptor overexpression.

TL;DR: It is reported that fibroblast growth factor 2 (FGF-2)-supplemented bone marrow stromal cell primary cultures display an early increase in telomere size followed by a gradual decrease, whereas in control cultures telomeres length steadily decreases with increasing population doublings.

TL;DR: In this article, the role of Smad proteins in terminal differentiation of fibroblasts was examined by transfection of cells, with expression vectors for the TGF-beta1 receptor-regulated Smads (R-Smads) or the co-Smad, Smad 4.

TL;DR: The data show the influence of overexpression on myoblast proliferation, differentiation, and apoptosis is extended to endogenous myostatin, and it appears that myogenin and p21 are essential targets of this growth factor.

TL;DR: Induction of the MMP-13 gene by TNF-α is mediated by ERK, p38, and JNK MAP kinases as well as AP-1 and NF-κB transcription factors, which may be beneficial for reducing cartilage breakdown by M MP-13 in arthritis.

TL;DR: In this paper, isolated mesenchymal stem cells (MSC) were shown to differentiate into cardiomyocytes, endothelial cells, and pericytes or smooth muscle cells by direct injection into the adult heart.

TL;DR: The capacity of zinc to inhibit protein tyrosine phosphatases (PTPs) is established as a cause for these effects and modulation of the insulin response by changes in intracellular zinc is demonstrated.

TL;DR: The distinct responses to DER activation in the different tissues depend upon combinatorial interactions with other signaling pathways and tissue-specific factors, at the level of target-gene regulation.

TL;DR: Observations indicated that the established tendon cell line possesses mesenchymal stem cell-like properties, suggesting the existence of mesenchyl stem cell in tendon tissue.

TL;DR: Ectosomes exposed active enzymes released by neutrophils upon degranulation and became specifically adherent to monocytic and endothelial cells, suggesting that neutrophil-derived ectosomes have unique characteristics that make them candidates for playing roles in inflammation and cell signaling.

TL;DR: The pathways by which tumor suppressors such as APC, p53, VHL, and BRCA1 cross the nuclear envelope and the impact of regulated nuclear import/export on protein function are reviewed.

TL;DR: There are several novel aspects to its structure, mechanism of action, and biology including the spectrum of ligands that activate ErbB-4, the presence of functionally distinct isoforms, a proteolytic processing pathway to the nucleus, and the capacity to induce a spectrum of cellular responses such as mitogenesis, differentiation, growth inhibition, and survival.

TL;DR: The ability of c-Myc to repress transcription of growth arrest genes may contribute to its potential to promote proliferation and oncogenesis.

TL;DR: Clinical studies already suggest that both approaches, either alone or in combination with standard anticancer therapies, are well tolerated and can induce clinical responses and tumor stabilization in a variety of common carcinomas.

TL;DR: It is concluded that CA IX has a capacity to modulate E-cadherin-mediated cell adhesion via interaction with β-catenin, which could be of potential significance in hypoxia-induced tumor progression.

TL;DR: Analysis of the effect of inorganic arsenic on Nrf2 expression and localization in HaCaT cells found that arsenic enhanced cellular expression of NRF2 at the transcriptional and protein levels and activated expression ofNrf2-related genes in these cells, and indicated that H(2)O(2), rather than *O( 2)(-), is the mediator of nuclear N RF2 accumulation.

TL;DR: In this article, the authors used modified Boyden chambers to test the effect of heparin-binding glycoprotein (CHI3L1) on vascular smooth muscle cell adhesion and migration.

TL;DR: Results show that SMC phenotype and function can be modulated using biochemical stimulation in vitro, but that the effects produced are dependent on the nature of the extracellular matrix.

TL;DR: The dominant negative form of FADD and z-VAD-fmk inhibited caspase-8, caspas-9, Bid processing, cytochrome c release, and DNA fragmentation induced by ER stress, suggesting that caspasing-8 and caspases-9 are the main caspased involved in ER stress-mediated apoptosis of P19-36/12 (-) cells.

TL;DR: Using microarray analysis, multiple genes regulated by the increase in phosphate associated with osteoblast differentiation are revealed, adding to the understanding of the intricate communication between osteoblasts and their extracellular environment.

TL;DR: It is demonstrated that cAMP elevated the barrier function of tight junctions in porcine blood-brain-barrier endothelial cells in PKA-dependent and -independent manners, and this findings indicate that both CAMP-induced phosphorylation of claudin-5 immunoprecipitates and cAMP-dependent but PKA -independent induction of claudi-5 expression could be involved in promotion of tight-junction function in endothelial Cells.

TL;DR: Analysis of skin fibroblasts from a patient with type 1B limb-girdle muscular dystrophy and from her deceased newborn grandchild carrying a heterozygous +/mut and a homozygous (mut/mut) nonsense Y259X mutation demonstrate that lamin C, like lamin A, interacts in vivo directly with nesprin-1alpha and with emerin and that lamination A or C is sufficient for the correct anchorage ofEmerin and n