Showing papers in "Experimental Hematology in 1999"

TL;DR: The osteoclast is a hematopoietic cell derived from CFU-GM and branches from the monocyte-macrophage lineage early during the differentiation process, and current research is beginning to delineate the signaling pathways involved in osteoclastic bone resorption and osteoc last formation in response to cytokines and hormones.

TL;DR: Evidence suggests that non-Stat pathways play a critical role in the generation of signals for both Type I and Type II interferons and may be the primary mediators of their growth inhibitory effects on hematopoietic cells.

TL;DR: It is demonstrated that the marrow stromal microenvironment is seriously and irreversibly damaged after BMT, and donor cells do not contribute to reconstitute the marrow microenvironment, whose residual CFU-fs remain of host origin.

TL;DR: Differences in the relative numbers of mature lymphoid, myeloid, and erythroid progeny produced by CRU from different ontologic sources were seen and evidence of a transplantable human lymphoid-restricted cell present throughout ontogeny was obtained.

TL;DR: It is found that MSCs isolated from recipients of allogeneic HSC transplantation are not of donor genotype and have persistent phenotypic defects despite successful donor type hematopoietic engraftment, raising the possibility that M SCs may be useful in the treatment of storage disorders.

TL;DR: It is demonstrated that although c-kit derived from canine MCT did not contain the previously described activating point mutations, 5 of the 11 tumors analyzed possessed novel mutations consisting of tandem duplications involving exons 11 and 12, suggesting that these mutations may contribute to the development or progression of canine M CT.

TL;DR: Data indicate that SD/01 represents an efficacious novel form of Filgrastim with actions sustained for between one and two weeks from a single injection, which would include a prolonged duration of action sufficient to cover a complete single course of chemotherapy.

TL;DR: Long-term incubation of NK cells with rHsp70 protein enhances not only the proliferative but also the cytolytic response against Hsp70-expressing tumor cells, indicating that the stimulatory effect is not strictly dependent on IL-2.

TL;DR: It is shown that multiple mechanisms are involved in NK cell lysis of breast cancer targets, that none of the targets are inherently resistant to killing, and that two distinct mechanisms of ADCC can target immunotherapy to breast cancer cells.

TL;DR: Stromal cotransplantation of stroma resulted in increased donor cell engraftment in the BM and led to significantly increased levels of donor hematopoiesis for over 30 months after transplant.

TL;DR: The large number of chemokine with suppressive activity is hypothesized to reflect the different cell, tissue, and organ sites of production of these chemokines and the need to control stem/progenitor cell proliferation in different organ sites throughout the body.

TL;DR: This review will focus on the events leading to the development of the early yolk sac lineages and the transition to multilineage hematopoiesis in the mouse.

TL;DR: Flow cytometric analysis showed that the patient's monocytes could not bind a monoclonal antibody specific for the IFNgamma-receptor, and analysis of genomic DNA revealed the presence of a G to T transition at the 5'end of the splice consensus sequence of intron 3, which explains the absence of exon 3.

TL;DR: Changes in adhesion receptor expression and function with cytokines and during cell cycle transit may be critical to stem cell homing and engraftment after transplantation, as multiple receptors could be involved in the process of rolling, attachment to endothelium, endothelial transmigration, and migration within the marrow space.

TL;DR: A novel culture system in which the murine stromal cell line HESS-5 dramatically supports the rapid expansion of cryopreserved CB-PPC in synergy with TPO/FL-2 is established, indicating that this xenogeneic coculture system, in combination with human cytokines, can rapidly generate PPC from cryop Reserved CB.

TL;DR: Analysis of retroviral transduction efficiency confirmed the increase of transgene expression from MPB primitive hematopoietic progenitors assayed after stromal culture was fivefold, validating the usefulness of multiparameter analysis of short-term cultures for survival and replication of CD34+ Thy-1+ cells.

TL;DR: The infusion of lymphocytes from the original marrow donor (donor lymphocyte infusion [DLI]) reinduces complete remission in a high percentage of patients with chronic myeloid leukemia who relapse after allogeneic stem cell transplant and is probably the best initial approach to their management.

TL;DR: All HSC from BALB mice seemed to have equal functional levels at a given stage of life and were gradually exhausted simultaneously through development and aging, suggesting that all HSC are functionally homogeneous at any particular time point during development or aging.

TL;DR: The results indicate the existence of a CT lifecycle in human mononuclear cells and warrant further studies to investigate the role of adrenergic autoregulatory mechanisms in modulation of the immune response and in the pathogenesis of diseases involving the immune system.

TL;DR: A novel adhesive interaction with collagen IV, reminiscent of that described for CML progenitors, may have a role in the extramedullary localization of FL hematopoiesis or its developmental stage-specific regulation by its microenvironment.

TL;DR: It is demonstrated that transplantable hematopoietic stem cells in human CB can be reproducibly transduced at high efficiency using a 6-day period of culture in a retrovirus-containing medium with three different flt3-ligand (FL)-containing cytokine combinations.

TL;DR: In this paper, the authors compared the kinetic recovery of lymphocytes and their subsets in two groups of patients submitted to allogeneic peripheral blood progenitor cell transplantation (allo-PBT): those receiving lymphocyte-depleted leukaphereses by positive selection of CD34 + cells (group 1, n=18) and those receiving unmanipulated leukAPhereses (group 2, n = 15).

TL;DR: In this article, the authors compared spontaneous and SDF-1-induced migration of CD34+ cells from bone marrow (BM), peripheral blood (PB), and cord blood (CB) across Transwell filters.

TL;DR: Elevated neutrophil levels can be maintained for extended periods following single administration of high-molecular-weight PEG-NTG, and increasing the extent of PEGylation also increases in vivo activity.

TL;DR: The results demonstrate that the TCPB matrix facilitates and enhances HPC maintenance and leads to a 1.5-fold expansion of HPC numbers following 1 week in culture and a 6.7-fold increase in colony-forming ability following 6 weeks in culture in the absence of exogenous cytokines.

TL;DR: The long-term repopulating ability of CD34+Lin- vs CD34-Lin- cells was compared directly using syngeneic murine bone marrow transplantation and it is confirmed that both populations contain hematopoietic stem cells.

TL;DR: It is shown that preterm CB compared with term CB is richer in hematopoietic progenitors, and that precursors from pretermCB can be extensively expanded ex vivo, which may have implications for the development of transplantation and gene transfer strategies targeting circulating fetal stem cells.

TL;DR: Analysis of serial differential cell counts from 45 congenital, idiopathic, and cyclic neutropenic patients before and during treatment with recombinant human G-CSF shows that the occurrence of significant cycling in the absolute neutrophil count of neutropenics not classified as cyclical is much more prevalent than had been previously thought, and that not all the patients classification as cyclic show significant ANC periodicity.

TL;DR: Analysis of the expression of proteins of the JAK/STAT pathway in normal myeloid cells at three stages of maturation revealed a strong expression of all proteins in CD34+ cells, whereas the level of the proteins was significantly lower in granulocytic precursors and mature neutrophils.

TL;DR: P phenotypic analysis of the CD34+CD109- cells in FBM, adult bone marrow (ABM) and cytokine-mobilized peripheral blood (MPB) demonstrated that this subset comprises lymphoid-committed progenitors, predominantly of the B-cell lineage.