Showing papers in "Experimental Parasitology in 2001"

TL;DR: A simple density gradient centrifugation method is described, which allows the rapid purification of infective metacyclic parasites from both wild-type and LPG-deficient Leishmania major and suggests that this method is applicable to other LeishMania species including L. donovani.

TL;DR: SSCP had advantages over the other methods employed for investigation of sequence variation within the species L. donovani and confirmed that SSCP was able to detect genetic diversity at the level of a single nucleotide.

TL;DR: There is genetic diversity within E. histolytica isolates from an endemic population as reflected in serine-rich E. historicica protein gene polymorphism, and the implications for the immunoprophylaxis of amebiasis require further study.

TL;DR: This study compares aspects of the superoxide, nitric oxide and prophenoloxidase pathways in Rhodnius prolixus hemolymph, measured in parallel, in response to Trypanosoma rangeli inoculation to indicate that both NADPH oxidase andNitric oxide synthase activity may be involved in the immune response of R. prolIXus to infection by T. rangela.

TL;DR: The results suggest that, among the different isotypes present in the serum of semi-immune individuals, parasite antigen-specific IgG3 in particular may play an important role in controlling parasitemia via an ADCI mechanism involving monocyte- derived mediators.

TL;DR: Investigation of phagocytic activity and the production of reactive oxygen species (ROS) by hemocytes from the cattle tick Boophilus microplus indicates that tick hemocytes are able to produce ROS during the phagocytetic process similarly to vertebrate phagocytes.

TL;DR: Use of crude preparations from field-blood samples collected on both filter paper and glass slides as DNA template suggested that this method could be useful for the diagnosis of T. vivax in large epidemiological studies.

TL;DR: This paper shows that, together, flow cytometry and microscopy are quick, sensitive, and easily reproducible methods to describe the effects of drugs on parasites.

TL;DR: A complementary DNA encoding the variant surface glycoprotein (VSG) of Trypanosoma evansi Rode Trypanozoon antigenic type (RoTat)1.2, currently used for experimental serological diagnosis of T.evansi infection in livestock, was cloned as a recombinant plasmid and sequenced and constructed to express the protein in Spodoptera frugiperda insect cells.

TL;DR: It is demonstrated that the cancer-associated O-glycosylated Tn antigen (GalNAc-O-Ser/Thr) is expressed by the cestode Echinococcus granulosus, and the first evidence of O- Glycosylation pathways in E. granulOSus is reported.

TL;DR: It is suggested that control of infection is associated with IFN-gamma and IL-12 production and migration of T cells and dendritic cells to the site of chronic parasitism.

TL;DR: The histopathological changes in these organs are compared with those studied in surra, human sleeping sickness disease, and African trypanosomiasis and possible mechanisms for these histologically changes in the visceral organs are discussed.

TL;DR: Production and characterization of rat monoclonal antibodies specific for the sexual-stage Pfs48/45 antigen for plasmodium falciparum is described.

TL;DR: The biochemical characterization of the parasite populations by molecular karyotype determined that the most frequent parasite isolated from patients belongs to clonet 39, and the clonets used as probes are the most prevalent ones in terms of transmission in the endemic areas studied.

TL;DR: The results favor the hypothesis that T. cruzi U.S. stocks were not imported at a historical time and are indigenous to the native fauna of the United States, and no evidence of hybrid genotypes was found in theUnited States.

TL;DR: The present findings indicate that the early phase of infection with T.cruzi induces rapid production, maturation, and activation of the monocyte/macrophage system so as to control T. cruzi replication, emphasizing the crucial role for macrophages in the rat resistance to Chagas disease.

TL;DR: Long PCR Amplification of Plasmodium falciparum DNA Extracted from Filter Paper Blots results in positive results for both EMT and EMT in flea infestation studies.

TL;DR: Molecular characterization of a full-length Tg MIC10 cDNA revealed that TgMIC10 lacks homology to any previously characterized proteins, although a homologue, NcMIC10, was identified in a closely related parasite, Neospora caninum.

TL;DR: Biochemical analyses of enzyme activities of highly resistant strains of both Trichomonas vaginalis and Tritrichomonas foetus suggest that altered gene expression of multiple hydrogenosomal proteins results in the modification of the organelle and leads to drug resistance.

TL;DR: It is suggested that Cs28GST may play significant physiological roles against bioreactive molecules and be a useful reagent for serodiagnosis of clonorchiasis.

TL;DR: Gene transfection experiments indicated that the link between the locus amplified and the resistance levels was not straightforward and possibly several mutations act together to lead to amphotericin B resistance.

TL;DR: The results demonstrate that the genome of adult S. mansoni is not methylated, and the methylation-dependent restriction endonuclease McrBC, which cleaves DNA containing methylcytosine and will not cleave unmethylated DNA, did not digest S.mansoni genomic DNA.

TL;DR: It is concluded that whereas some mutations producing changes in the amino acid sequence of the DHFR molecule may occur occasionally in clones or populations of P. falciparum grown in vitro in the presence of pyrimethamine, amplification of theDHFR gene following adaptation to growth in medium containing pyrimEThamine occurs as a regular feature.

TL;DR: The apparent decrease in potency of clinical-grade TBV25-28 in rabbits appears to reflect an enhancement in potency in a new formulation rather than simply a species difference in immunogenicity of TBV 25-28.

TL;DR: The data show that cryptic species within the T. brucei group can be distinguished by differences in the PCR-RFLP profile of the rDNA repeat, and suggests that human pathogenicity might have evolved secondarily.

TL;DR: Biomagnetic separation of contaminating host leukocytes from Plasmodium-infected erythrocytes in E.coli is distinguished by a high level of interference with the ability of the E. Coli to reprogram the nucleus of the leukocyte.

TL;DR: Results suggest that while WR243251 may inhibit respiration, its target of action probably differs from that of atovaquone, which may inhibit growth additively or with mild synergy.

TL;DR: Results indicate that P. vivax VK247 parasites are destroyed at different parasite stages during migration in An.

TL;DR: The thermoreceptive function of the AFD-class neurons associates this neuron pair with the host-finding process of the A. caninum infective larva and shows functional homology with the neurons of class AFD in C. elegans and in H. contortus.

TL;DR: Results indicate that this modified DD procedure is suitable for large-scale identification of developmentally regulated genes in the AT-rich Plasmodium genome.